JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (9): 44-48.

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Influence of hypoxia on expressions of relative factors of the Wnt/β-catenin signal pathway in neural stem cells in vitro

CUI Xiao-ping1, LIN Hang1, CHEN Jian-mei2， MU Jun-shan1， YE Jian-xin1， YIN Hong-bing1， YING Da-jun3   

  1. 1. Department of Neurology, Fuzhou General Hospital of Nanjing Military Area, Clinical Medical College of
    Fujian Medical University, Fuzhou 350025, China；
    2. Department of First Orthopaedics, Fuzhou General Hospital of Nanjing Military Area, Fuzhou 350025, China;
    3. Department of Anatomy, Third Military Medical University, Chongqing 400038, China
  • Received:2010-01-20 Online:2010-09-16 Published:2010-09-16

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Abstract:

 Objective    To observe expressions of relative factors of the Wnt/β-catenin signal pathway in neural stem cells (NSCs) cultured in vitro in normoxia(20%O2) and hypoxia (3±2%O2) and explore the mechanism of proliferation of NSCs in hypoxia. Methods    NSCs obtained from the hippocampus of a neonatal mouse were cultured in normoxia and hypoxia, then expressions of major factors of the Wnt/β-catenin pathway were detected by RT-PCR. After the luciferase reporter gene plasmid was transfected into NSCs by electroporation, responsive ability of the Wnt/β-catenin pathway to hypoxia was detected, and expressions of β-catenin and CyclinD1 in NSCs were detected by Western blot.  Results    The major factors in the Wnt/β-catenin signal pathway were detected in NSCs and the Wnt/β-catenin signal pathway was obviously responsive to hypoxia. The β-catenin and CyclinD1 protein levels were increased after hypoxia treatment compared with those in normoxia(P<0.05). Conclusion    The Wnt/β-catenin signal pathway may promote the proliferation of NSCs in hypoxia by increasing expressions of β-catenin and CyclinD1 in vitro.

Key words: Cell hypoxia; Stem cells; Signal transduction

CLC Number: 

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