JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (7): 1-7.doi: 10.6040/j.issn.1671-7554.0.2015.171

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Mechanism of HGF/PARP-1 signaling pathway regulating invasion and metastasis of ovarian cancer

LÜ Shuqing, WEI Wei, ZHANG Cancan, TIAN Yongjie   

  1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-02-10 Revised:2015-04-24 Online:2015-07-10 Published:2015-07-10

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Abstract: Objective To investigate the effects and signaling pathway of HGF-regulated PARP-1 expression on invasion and metastasis of ovarian cancer. Methods SKOV-3 cells were incubated with various concentrations of HGF to examine their invasive abilities by Transwell assay. Relative expression of PARP-1 after HGF treatment in SKOV-3 cells was analyzed by RT-PCR and Western blotting. SKOV3 cells were transfected with either negative control siRNA (NC-siRNA group) or PARP-1 siRNA (PARP1-siRNA group), and divided into different groups depending on different factors: blank control group; HGF group; PARP-siRNA group; HGF+PARP-siRNA group; NC-siRNA group; HGF+NC-siRNA group. The expression of PARP-1 in different groups was measured with Western blotting. The invasive abilities were examined with Transwell assay. The expression of MMP-2 was detected with ELISA. Results HGF promoted cell invasion in a concentration- and time-dependent manner in the SKOV-3 cell line. The expression of PARP-1 in SKOV-3 cells increased after HGF 40 ng/mL treatment for 24 hours. The expression of PARP-1 in PARP1-siRNA group was lower than that in NC-siRNA group (P<0.05). The transfection significantly reduced impacts of HGF on SKOV-3 cell invasion and the expression of MMP-2. Conclusion HGF promotes the invasion and metastasis of ovarian cancer. This effect may be related to the increased expression of MMP-2 which is mediated by PARP-1.

Key words: Invasion, Matrix metalloproteinase-2, Hepatocyte growth factor, Ovarian neoplams, Poly (ADP-ribose) polymerase-1

CLC Number: 

  • R737.31
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