Journal of Shandong University (Health Sciences) ›› 2017, Vol. 55 ›› Issue (12): 7-12.doi: 10.6040/j.issn.1671-7554.0.2017.150

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Expression of Cyr61 in the adipose tissue of obese mice induced by high fat diet

QING Li, HE Tianyi, QIAO Jingting, CUI Chen, HOU Xinguo, CHEN Li   

  1. Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Online:2017-12-20 Published:2022-09-27

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Abstract: Objective To investigate the expression and distribution of cysteine rich protein 61(Cyr61)in the adipose tissue of obese mice and its relationship with fat chronic inflammation. Methods A total of 30 male C57BL/6 mice were randomly divided into the normal diet group(NC group)and high fat diet group(HFD group). The HFD group was fed with high fat diet of 60% fat content to construct the obese mouse models. The NC group was fed with normal diet. After modeling, the body fat content of mice was measured, glucose tolerance and blood lipids were detected with intraperitoneal injection of glucose tolerance test(IPGTT), the expression and distribution of Cyr61 in the testis tissue were measured, and the expressions of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)mRNA in the adipose tissue were detected. Results The body weight, body fat content and fat tolerance of obese mice were significantly higher than those in NC group(P<0.05). The serum cholesterol and low density lipoprotein cholesterol were significantly higher than those in NC group(P<0.05). The expressions of TNF-α and IL-6 in the adipose tissue of obese mice were up-regulated(P<0.05). Both of the protein and mRNA levels of Cyr61 increased in the adipose tissue of HFD group(P<0.05), and Cyr61 was mainly distributed in the adipose tissue matrix. Conclusion Cyr61 expression, which is mainly located in the adipose tissue matrix, is significantly increased in the adipose tissue of obese mice, and is consistent with the expression of inflammatory factors, suggesting that it may be related to the occurrence of chronic inflammation.

Key words: Cysteine rich protein, Adipose tissue, Obesity, Inflammatory factor, Chronic inflammation

CLC Number: 

  • R723.14
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