JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (3): 49-53.doi: 10.6040/j.issn.1671-7554.0.2016.761

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Inhibition of human apical papilla cells on the activation of mononuclear cell THP-1 and its molecular mechanism

YAN Songhe1, GAO Lifen2, ZHAO Wei1, WANG Yan1,3   

  1. 1. School of Stomatology Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration;
    2. Department of Immunology, School of Basic Medical Sciences, Shandong University;
    3. VIP Center, Stomatological Hospital, Shandong University, Jinan 250012, Shandong, China
  • Received:2016-06-30 Online:2017-03-10 Published:2017-03-10

Abstract: Objective To investigate the effects of human apical papilla cells(HAPCs)on the mononuclear cell line THP-1 activation and the molecular mechanism in vitro. Methods HAPCs and THP-1 were co-cultured in Transwell system, and then the mRNA expressions of TNF-α, IL-12, IL-10 and TGF-β1 in THP-1 were detected with Real-time PCR. The expression of TNF-α in THP-1 3d after co-culture was determined with flow cytometry. The phosphorylation of Stat3 and Smad3 were tested with Western blotting. Results HAPCs inhibited the mRNA expressions of TNF-α and IL-12 in THP-1 and promoted the mRNA expressions of IL-10 and TGF-β1(P<0.05). The expression of TNF-α significantly decreased in THP-1 3 d after co-culture(P<0.05). HAPCs activated the Stat3 pathway in THP-1 after co-cultivation(P<0.05), but had no effect on the Smad3 pathway(P>0.05). Conclusion HAPCs may inhibit the activation of THP-1 through the Stat3 pathway.

Key words: Human apical papilla cells, Inflammatory factors, THP-1 cell

CLC Number: 

  • R781
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