Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (6): 64-70.doi: 10.6040/j.issn.1671-7554.0.2021.0076

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Expression and significance of serum miR-33a in patients with stable angina pectoris

ZHANG Mingming1, GAO Wei1, CUI Jia1, HUO Lijing1, HU Qingchuan1, LI Weihao2, WANG Chao3   

  1. 1. Department of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, Hebei, China;
    2. Department of Special Laboratory, the Second Hospital of Hebei Medical University, Shijiazhuang 050050, Hebei, China;
    3. Clinical Mdicine Research Center, Hebei General Hospital, Shijiazhuang 050051, Hebei, China
  • Published:2021-06-10

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Abstract: Objective To investigate the level and significance of serum miR-33a in patients with stable angina pectoris(SAP). Methods A total of 220 SAP patients treated during Jan. 2018 and Dec. 2019 were recruited as the SAP group, and 200 healthy cases were enrolled as control group. The SAP patients were divided into Ⅰ, Ⅱ, Ⅲ, Ⅳ according to the severity of the disease. The level of miR-33a in both groups was detected with real time PCR, and serum lipids and inflammatory factors were detected. Data in the GEO database was retrieved for bioinformatics analysis on the function of miR-33a. Results The level of serum miR-33a in the SAP group was significantly higher than that in control group(P<0.05). There were significant differences in miR-33a among patients with different severity of SAP(P<0.05). The levels of serum total cholesterol(TC), triglycerides(TG), low-density lipoprotein(LDLC), and very low-density lipoprotein(VLDL)were higher in the SAP group than in control group, while high-density lipoprotein(HDLC)level was lower(P<0.05). The serum inflammatory factors, including tumor necrosis factor(TNF-α), high-sensitivity C-reactive protein(hsCRP), and interleukin-6(IL-6), in SAP group were higher than those in control group(P<0.05). Level of serum miR-33a in SAP patients was positively correlated with LDL and VLDL, but was negatively correlated with HDL(P<0.05). Level of serum miR-33a in SAP patients was positively correlated with TNF-α and IL-6(P<0.05). Bioinformatic analysis showed that miR-33a was involved in regulating transcriptome genes, nucleic acid metabolism, signal transduction, cell growth, amino acid transport and other processes. Conclusion The expression of miR-33a increases in SAP patients, which is related to some lipids and inflammatory factors. miR-33a may play a role in the progression of SAP by regulating these indicators via complex molecular network mechanism.

Key words: Stable angina pectoris, miR-33a, Serum lipids, Inflammatory factors

CLC Number: 

  • R541.4
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