Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (6): 71-75.doi: 10.6040/j.issn.1671-7554.0.2020.1648

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Association between MTHFR polymorphism and serum homocysteine in patients with unstable angina pectoris

ZHANG Lin1, WANG Yan2, MA Xu2, FAN Xuesong2, YUAN Hui2   

  1. 1. Department of Clinical Laboratory, Beijing Forestry University Hospital, Beijing 100083, China;
    2. Department of Clinical Laboratory, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
  • Published:2021-06-10

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Abstract: Objective To investigate the relationship between methylenetetrahydrofolate reductase(MTHFR)gene polymorphism and serum homocysteine(Hcy)level in patients with unstable angina pectoris(UAP). Methods A total of 243 UAP patients(UAP group)and 269 healthy controls were selected during Jan. 2019 and Jun. 2019 in Beijing Anzhen Hospital. The serum Hcy level of the two groups was detected with double reagent circulating enzyme method, and the MTHFR gene polymorphism was detected with polymerase chain reaction and fluorescence method. The differences in serum Hcy level and MTHFR gene polymorphism between the two groups were compared with t test. MTHFR gene polymorphisms were classified into CT, CC and TT according to PCR products. Results The overall level of serum Hcy in UAP group was significantly higher than that in healthy control group ［(13.37±7.72)μmol/L vs(11.50±5.05)μmol/L, P<0.05］. The Hcy level of CT genotype in UAP group was significantly higher than that in healthy control group ［(13.08±7.33)μmol/L vs(10.78±2.45)μmol/L, P<0.05］. There was no difference in the distribution frequency of MTHFR C677T genotype(P>0.05), but the distribution frequency of T allele in the UAP group was higher than that in healthy control group(59.1% vs 52.5%, P<0.05). Conclusion The level of Hcy in UAP patients is significantly higher than that in healthy controls. There is no significant correlation between MTHFR C677T gene polymorphism and UAP, but T allele may be related to UAP.

Key words: Unstable angina pectoris, Methylenetetrahydrofolate reductase, Homocysteine, Gene polymorphism, Angina pectoris

CLC Number: 

  • R446
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