JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (2): 21-26.doi: 10.6040/j.issn.1671-7554.0.2015.778

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Enalapril inhibits homocysteine-induced smooth muscle cell dedifferentiation and its signal pathway

YANG Bo1,2, LI Ping1, MENG Liping2,3, ZHOU Changzuan2,3, PAN Sunlei2,3, CHI Jufang2, GUO Hangyuan2,3   

  1. 1. Department of Cardiology, Laiwu Central Hospital of Shandong Energy Xinwen Mining Group, Laiwu 271103, Shandong, China;
    2. Department of Cardiology, Shaoxing Peoples Hospital, Shaoxing 312000, Zhejiang, China;
    3. Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
  • Received:2015-08-18 Online:2016-02-10 Published:2016-02-10

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Abstract: Objective To explore whether enalapril could inhibit homocysteine(Hcy)-induced dedifferentiation of rat aortic vascular smooth muscle cells(VSMCs)and its potential mechanism. Methods The primary culture and identification of rat VSMCs was conducted and VSMCs in passage 4-7 were divided into 4 groups: control group, Hcy(100 μmol/L)group, Hcy+enalapril group, and Hcy+LY-294002 group. The proliferation and migration of VSMCs were detected using MTT, transwell chambers and wound healing, respectively. The morphology of VSMCs was observed with ICC. The expressions of SM-actin, SM-MHC, Calponin, OPN and p-AKT in VSMCs of every group were investigated using Western blotting. Results Compared with those of the control group, the proliferation and migration ability of VSMCs were significantly increased, the expressions of SM-MCH and Calponin were increased, while OPN was decreased in the Hcy group(P<0.01). There was no significant difference in the expression of SM-actin 山 东 大 学 学 报 (医 学 版)54卷2期 -杨博,等.依那普利抑制大鼠血管平滑肌细胞表型转化及可能的信号通路 \=-among the groups. Compared with those of the Hcy group, the proliferation and migration ability of VSMCs were significantly decreased, the expression of SM-MCH and calponin increased, while OPN and pAKT expressions were decreased in enalapril and LY-294002-treated groups(all P<0.01). Conclusion Hcy can induce, while enalapril can inhibit the dedifferentiation of VSMCs, by inhibiting the PI3K/p-AKT signal pathway.

Key words: Vascular smooth muscle cell dedifferentiation, LY-294002, Enalapril, Homocysteine

CLC Number: 

  • R543.3
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