Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (3): 94-98.doi: 10.6040/j.issn.1671-7554.0.2019.1405

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Expressions of miR-122 and miR-33a in patients with type 2 diabetes complicated with coronary artery disease

JIN Haiyan1, ZHANG Yan1,2, MA Xiaoli1, HAN Yu1,3, ZHAO Huichen1, LIU Yuantao1, ZHANG Yuchao1   

  1. 1. Department of Endocrinology, Qingdao Municipal Hospital, Qingdao 266011, Shandong, China;
    2. Department of Endocrinology, Chifeng Municipal Hospital, Chifeng 024000, Inner Mongolia, China;
    3. Nanyang Second General Hospital, Nanyang 473000, Henan, China
  • Online:2020-03-10 Published:2022-09-27

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Abstract: Objective To investigate the expressions and diagnostic significance of serum miR-122 and miR-33a in patients with type 2 diabetes mellitus(T2DM)complicated with coronary artery heart disease(CAD). Methods Fast bloods samples were collected from 32 healthy volunteers(NC group), 32 T2DM patients(T2DM group), 32 CAD patients(CAD group)and 32 patients with T2DM and CAD(T2DM+CAD group). The expressions of miR-122 and miR-33a were detected with real-time quantitative PCR. Specificity and sensitivity of the association between miR-122 in T2DM and CAD were evaluated with receiver operating characteristic(ROC)and area under the curve(AUC). Results Compared with the NC group, the other three groups had significantly higher expressions of miR-122(P<0.05). Compared with T2DM group, the CAD and T2DM+CAD groups had significantly higher expression of miR-122(P<0.05). The AUC of miR-122 in CAD group and T2DM+CAD group was 0.72±0.05 and 0.77±0.04, respectively. There were no significant differences in the miR-33a expression among the four groups(P>0.05). Conclusion Serum miR-122 can serve as a good diagnostic marker for T2DM complicated with CAD.

Key words: miR-122, miR-33a, Type 2 diabetes mellitus, Coronary artery heart disease

CLC Number: 

  • R574
[1] 中华医学会糖尿病学分会. 中国2型糖尿病防治指南(2013年版)[J]. 中国糖尿病杂志, 2014, 88(7): 1227-1245.
[2] Hu DY, Pan CY, Yu JM. The relationship between coronary artery disease and abnormal glucose regulation in China: the China Heart Survey [J]. Eur Heart J, 2006, 27(21): 2573-2579.
[3] Amsterdam EA, Wenger NK, Brindis RG, et al. 2014 AHA/ACC guideline for the management of patients with Non-ST-Elevation acute coronary syndromes: a report of the American college of cardiology/American heart association task force on practice guidelines [J]. J Am Coll Cardiol, 2014, 64(24): e139-228.
[4] Lewis AP, Jopling CL. Regulation and biological function of the liver-specific miR-122 [J]. Biochem Soc Trans, 2010, 38(6): 1553-1557.
[5] Najafi-Shoushtari SH, Kristo F, Li Y, et al. MicroRNA-33 and the SREBP host genes cooperate to control cholesterol homeostasis [J]. Science, 2010, 328(5985): 1566-1569.
[6] Dávalos A, Goedeke L, Smibert P, et al. miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling [J]. Proc Natl Acad Sci USA, 2018, 108(22): 9232-9237.
[7] Sacco J, Adeli K. MicroRNAs: emerging roles in lipid and lipoprotein metabolism [J]. Curr Opin Lipidol, 2012, 23(3): 220-225.
[8] Poornima IG, Parikh P, Shannon RP. Diabetic cardiomyopathy: the search for a unifying hypothesis [J]. Circ Res, 2006, 98(5): 596-605.
[9] Htay T, Soe K, Lopez-Perez A, et al. Motality and cardiovascular disease in type 1 and type 2 diabetes [J]. Curr Cardiol Rep, 2019, 21(6): 45-51.
[10] Zlotorynski E. Insights into the kinetics of microRNA biogenesis and turnover [J]. Nat Rev Mol Cell Biol, 2019, 20(9): 511.
[11] Reichholf B, Herzog VA, Fasching N, et al. Time-resolved small RNA sequencing unravels the molecular principels of miroRNA homeostasis [J]. Mol Cell, 2019, 75(4): 756-768.
[12] Rottiers V, Naar AM. MicroRNAs in metabolism and metabolic disorders [J]. Nat Rev Mol Cell Biol, 2012, 13(4): 239-250.
[13] Giral H, Kratzer A, Landmesser U. MicroRNAs in lipid metabolism and atherosclerosis [J]. Best Prac Res Clin Endocrinol Metab, 2016, 30(5): 665-676.
[14] Koyama S, Horie T, Nishino T, et al. Identification of differential roles of microRNA-33a and -33b during atherosclerosis progression with genetically modified mice [J]. J Am Heart Assoc, 2019, 8(13): e012609.
[15] Esau C, Davis S, Murray SF, et al. miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting [J]. Cell Metab, 2006, 3(2): 87-98.
[16] Sedgeman LR, Michell DL, Vickers KC. Integrative roles of microRNAs in lipid metabolism and dyslipidemia [J]. Curr Opin Lipidol, 2019, 30(3): 165-171.
[17] Iliopoulos D, Drosatos K, Hiyama Y, et al. MicroRNA-370 controls the expression of MicroRNA-122 and Cpt1α and affects lipid metabolism [J]. J Lipid Res, 2010, 51(6): 1513-1523.
[18] Valdmanis PN, Kim HK, Chu K, et al. miR-122 removal in the liver activates imprinted microRNAs and enables more effective microRNA-mediated gene repression [J]. Nat Commun, 2018, 9(1): 5321-5329.
[19] Naderi M, Pazouki A, Arefian E, et al. Two triacylglycerol pathway genes, CTDNEP1 and LPIN1, are down-regulated by has-miR-122-5p in hepatocytes [J]. Atch Iran Med, 2017, 20(3): 165-171.
[20] Wen J, Friedman JR. miR-122 regulates hepatic lipid metabolism and tumor suppression [J]. J Clin Invest, 2012, 122(8):2773-2776.
[21] Tsai WC, Hsu SD, Hsu CS, et al. MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis [J]. J Clin Invest, 2012, 122(8): 2884-2897.
[22] Willeit P, Skroblin P, Moschen AR, et al. Circulating microRNA-122 is associated with the risk of new-onset-metabolic syndrome and type 2 diabetes [J]. Diabetes, 2017, 66(2): 347-357.
[23] Castano C, Kalko S, Novials A, et al. Obesity-associated exosomal miRNAs modulate glucose and lipid metabolism in mice [J]. Diabetes, 2018, 115(48): 12158-12163.
[24] 吴霞, 王学法, 金鑫. 急性冠脉综合征患者血浆miR-122和miR-3149 表达的临床意义[J]. 山东医药, 2017, 57(4): 88-90.
[25] Shah R, Murthy V, Pacold M, et al. Extracellular RNAs are associated with insulin resistance and metabolic phenotypes [J]. Diabetes Care, 2017, 40(4): 546-553.
[26] Gao W, He HW, Wang ZM, et al. Plasma levels of lipometabolism-related miR-122 and miR-370 are increased in patients with hyperlipidemia and associated with coronary artery disease [J]. Lipids Health Dis, 2012, 11: 55-62. doi: 10.1186/1476-511X-11-55
[27] Najafi-Shoushtari SH, Kristo F, Li Y, et al. MicroRNA-33a and the SREBP host genes cooperate to control cholesterol homeostasis [J]. Science, 2010, 328(5985): 1566-1569.
[28] Platania CBM, Maisto R, Trotta MC, et al. Retina and circulating miRNA expression patterns in diabetic retinopathy: an in silic and in vivo approach [J]. Br J Pharmacol, 2019, 176(13): 2179-2194.
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