Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (3): 99-106.doi: 10.6040/j.issn.1671-7554.0.2019.1396

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A mechanism study on the association of type 2 diabetes and high-density lipoprotein

TANG Bo1,2, SHAO Jing3, CUI Jing4, SUN Jianping1,4   

  1. 1.Nutrition and Food Hygiene, School of Public Health, Qingdao University, Qingdao 266021, Shandong, China;
    2. Department of Otolaryngology, Peoples Hospital of Pingyi County, Linyi 273300, Shandong, China;
    3. Family Planning Service Center for Maternal and Child Health in Licang District, Qingdao 266041, Shandong, China;
    4. Qingdao Municipal Center for Disease Control and Prevention, Qingdao Institute of Preventive Medicine, Qingdao 266033, Shandong, China
  • Online:2020-03-10 Published:2022-09-27

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Abstract: Objective To explore the molecular mechanism of type 2 diabetes(T2DM)and high-density lipoprotein(HDL)by bioinformatics. Methods Genes of T2DM and HDL were obtained from GEO database and Comparative Toxicogenomics Database(CTD)to screen the same differentially expressed genes(DEGs). Gene function enrichment analysis of gene ontology(GO)and KEGG pathway in DEGs were analyzed with DAVID online software. Protein-protein interaction(PPI)network about DEGs was analyzed with String online software and MCODE of Cytoscape. 山 东 大 学 学 报 (医 学 版)58卷3期 -唐博,等.2型糖尿病发病与高密度脂蛋白关系的机制研究 \=- Results There were 13 DEGs associated with T2DM and HDL, which played a role in cholesterol metabolic process. The DEGs were involved in HIF-1 signaling pathway, Toll-like receptor signaling pathway and cGMP-PKG signaling pathway. All 13 DEGs were involved in the PPI network, which had 27 edges, with average protein node degree of 4.15 and local clustering coefficient of 0.74. The PPI network had a significant difference (P<0.001). The PPI network associated with T2DM and HDL was composed of 6 node proteins, including LEPR, MAPK3, AKT1, NOS3, APOE and SCARB1. Conclusion The pathogenesis of T2DM involves abnormal HDL through cholesterol metabolic process, HIF-1 signaling pathway, Toll-like receptor signaling pathway and cGMP-PKG signaling pathway. Further studies are needed to clarify the possible causal association between lipid and T2DM.

Key words: Type 2 diabetes mellitus, High-density lipoprotein, Gene ontology analysis, KEGG analysis, Protein-protein interaction network

CLC Number: 

  • R587.1
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