JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (2): 48-51.doi: 10.6040/j.issn.1671-7554.0.2014.768

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Correlations among RhoA/ROCK signaling pathway, blood pressure variability and carotid artery intima-media thickness in essential hypertensive patients

XU Zhongyang, WANG Liqi, XU Zhenxing, ZHAO Qian, ZHU Shiming   

  1. Department of Cardiology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2014-10-30 Published:2015-02-10

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Abstract: Objective To investigate the association of the blood pressure variability (BPV) and carotid intima-media thickness (IMT) with the Rho/ROCK signaling pathway in essential hypertensive patients. Methods A total of 73 hypertensive patients were divided into two groups based on serum level of ROCK1: group with high level of ROCK1 (n=37) and group with low level of ROCK1 (n=36), and 40 healthy subjects were selected as the control group. The serum level of ROCK1 was measured with Human Rock1 Elisa kit and microplate reader. The mean blood pressures (systolic and diastolic) and the standard deviations of 24 h, daytime and nighttime (24hSSD, dSSD, nSSD, 24hDSD, dDSD and nDSD) were monitored. The carotid artery IMT was determined with color Doppler ultrasound monitor. Results The serum level of ROCK1 in hypertensive group was higher than that of the control group (P<0.05). Compared with the group with low ROCK1 level, the 24hSSD, dSSD and nSSD were significantly increased in the group with high ROCK1 level (P<0.05). The carotid artery IMT in hypertensive patients was thicker than that of control group (P<0.05). The carotid artery IMT of the group with high ROCK1 level was thicker than that of the group with low ROCK1 level (P<0.05). The serum level of ROCK1 showed significant positive correlations with 24hSSD, dSSD, nSSD and carotid artery IMT in hypertensive patients (P<0.05). Conclusion The BPV is closely correlated with expression of Rho/ROCK signaling pathway in hypertensive patients. Overexpression of Rho/ROCK signalingpathway plays a key role in the progression of carotid artery IMT and atherosclerosis.

Key words: RhoA/ROCK, Intima-media thickness, Hypertension, Blood pressure variability, Atherosclerosis, Ambulatory blood pressure monitoring

CLC Number: 

  • R544.1
[1] 宫丽丽, 方莲花, 杜冠华. 心血管疾病治疗的新靶点—Rho激酶[J]. 中国药学杂志, 2008, 43(1):1-4.
[2] Wibberley A, Chen Z, Hu E, et al. Expression and functional role of Rho-kinase in rat urinary bladder smooth muscle[J]. Br J Pharmacol, 2003, 138(5):757-766.
[3] Zhou Q, Gensch C, Liao JK. Rho-associated coiled-coil-forming kinases (ROCKs): potential targets for the treatment of atherosclerosis and vascular disease[J]. Trends Pharmacol Sci, 2011, 32(3):167-173.
[4] Mueller BK, Mack H, Teusch N. Rho-kinase: a promising drug target for neurological disorders[J]. Nat Rev Drug Discov, 2005, 4(5):387-398.
[5] Gao HC, Zhao H, Zhang WQ, et al. The role of the Rho/Rock signaling pathway in the pathogenesis of acute ischemic myocardial fibrosis in rat models[J]. Exp Ther Med, 2013, 5(4):1123-1128.
[6] 蔡茜, 吴尚洁, 赵雪峰. 慢性阻塞性肺疾病相关性肺动脉高压患者外周血单核细胞Rho激酶水平测定[J]. 中南大学学报:医学版, 2012, 37(5):453-457. CAI Qian, WU Shangjie, ZHAO Xuefeng. Measurement of Rho-kinase in peripheral blood monocytes in patients with pulmonary arterial hypertension related to chronic obstructive pulmonary diseases[J]. J Cent South Univ:Med Sci, 2012, 37(5):453-457.
[7] Nakagawa O, Fujisawa K, Ishizaki T, et al. ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice[J]. FEBS Lett, 1996, 392(2):189-193.
[8] Somlyo AP, Somlyo AV. Ca2+ sensitivity of smooth muscle and nonmuscle myosin II; modulated by G proteins, kinases, and myosin phosphatase[J]. Physiol Rev, 2003, 83(4):1325-1358.
[9] Somlyo AP, Somlyo AV. Signal transduction by G proteins, Rhokinase and protein phosphatase to smooth muscle and non-musclemyosin II[J]. J Physiol, 2000, 522(2): 177-185.
[10] Mukai Y, Shimokawa H, Matoba T. Involvement of Rho-kinase in hypertensive vascular disease: a novel therapeutic target in hypertension[J]. FASEB J, 2001, 15(6):1062-1064.
[11] Ito K, Hirooka Y, Sakai K, et al. Rho/Rho-kinase pathway in brain stem contributes to blood pressure regulation via sympathetic nervous system: possible involvement in neural mechanisms of hypertension[J]. Circ Res, 2003, 92(12):1337-1343.
[12] Mancia G, Grassi G. Mechanisms and clinical implications of blood pressure variability[J]. J Cardiovasc Pharmacol, 2000, 35(4):15-19.
[13] 胡建新, 胡宪珍, 张润香, 等. 血压变异性与血浆一氧化氮、内皮素含量的关系[J]. 江西医学院学报, 2004, 44(5):38-39. HU Jianxin, HU Xianzhen, ZHANG Runxiang, et al.The relationship between blood pressure variability and the plasma levels of nitric oxide and endothelin-1[J]. Acta Academiae Medicinae Jiangxi, 2004, 44(5):38-39.
[14] Shibasaki M, Durand S, Davis SL, et al. Endogenous nitric oxide attenuates neutrally mediated cutaneous vasoconstriction[J]. J Physiol, 2007, 585(2):627-634.
[15] Alexander J S. Rho, tyrosine kinase, Ca2+, and junctions in endothelial hyperpermeability[J]. Circ Res, 2000, 87(4):268-271.
[16] Worthylake RA, Lemoine S, Watson JM, et al. RhoA is required for monocyte tail retraction during transendothelial migration[J]. J Cell Biol, 2001, 154(1):147-160.
[17] 刘春南, 余首先, 赵晓宇. Rho激酶在心血管疾病中新进展及Rho激酶抑制剂的应用[J]. 心脏杂志, 2012, 24(4):521-524. LIU Chunnan, YU Shouxian, ZHAO Xiaoyu, et al. Rho kinase in cardiovascular diseases and applications for Rho kinase inhibition:Current progress[J]. Chin Heart J, 2012, 24(4):521-524.
[18] Sander D, Kukla C, Klingelhfer J, et al. Relationship between circadian blood pressure patterns and progression of early carotid atherosclerosis: A 3-year follow-up study[J]. Circulation, 2000, 102(13):1536-1541.
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