Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (12): 74-78.doi: 10.6040/j.issn.1671-7554.0.2025.0692

• Clinical Medicine • Previous Articles    

Effet of miR-125a-3p on the proliferation of rheumatoid arthritis synovial fibroblasts

SONG Huishu1, SUN Zhijian2, ZHANG Qiuting1, MI Xue1, ZHANG Ni1, WANG Zhilun1, YIN Zhe1, HAO Xuexi1, WANG Yingxue1   

  1. 1. Department of Rheumatology, The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Nanjing University, Nanjing 210008, Jiangsu, China
  • Published:2025-12-19

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Abstract: Objective To investigate the effects of miR-125a-3p on the proliferation capacity and inflammatory factor secretion levels in rheumatoid arthritis synovial fibroblasts(RA-FLS). Methods Synovial tissues were aseptically obtained from 25 patients with rheumatoid arthritis(RA)after joint replacement surgery, and RA-FLS were isolated for experimental study. MiR-125a-3p mimics and miR-NC were transfected into RA-FLS via cell transfection, and the cultured cells were divided into Control, miR-NC, and miR-125a-3p mimics groups. The expression of miR-125a-3p in each group was detected by real-time quantitative PCR(RT-qPCR). Cell counting kit-8(CCK-8)was used to determine the cell proliferation capacity, and enzyme-linked immunosorbent assay(ELISA)was employed to detect the levels of tumor necrosis factor α(TNF-α), interleukin-6(IL-6), and interleukin-1β(IL-1β)in the cell culture supernatant. Results The cell viability of RA-FLS and the levels of TNF-α, IL-1β, and IL-6 in the cell culture supernatant in the miR-125a-3p mimics group were significantly lower than those in the miR-NC group(P<0.001). Conclusion MiR-125a-3p inhibits the proliferation of RA-FLS and the secretion of inflammatory factors, potentially serving as a new target for studying the pathogenesis of RA.

Key words: Rheumatoid arthritis, Synovial fibroblasts, MicroRNA, MiR-125a-3p

CLC Number: 

  • R684.3
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