Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (1): 54-59.doi: 10.6040/j.issn.1671-7554.0.2019.1049

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Expression of microRNA519D and matrix metalloproteinase2 in placenta tissues of pre-eclampsia and its significance

ZHANG Weiyue1, LI Yaguang1, LIU Enling2, WANG Weijie1, WEI Jingwen1, JING Nan1   

  1. 1. Graduate School, North China University of Science and Technology, Tangshan 063210, Hebei, China;
    2. Department of Obstetrics and Gynecology, Tangshan Gongren Hospital, Tangshan 063000, Hebei, China
  • Published:2022-09-27

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Abstract: Objective To investigate the expressions of microRNA519D(miR-519d)and matrix metalloproteinase2(MMP2)in placental tissues of patients with pre-eclampsia(PE). Methods A total of 71 pregnant women in Tangshan Gongren Hospital from Nov. 2018 to May 2019 were divided into PE patients(PE group, n=16), severe PE patients(severe PE group, n=20), and normal pregnant women(control group, n=35). The expression levels of miR-519d and MMP2 were detected by real-time quantitative PCR, immunohistochemistry and Western blotting methods. Results The expression levels of miR-519d in placental tissues of control group, PE group and severe PE group were 0.992±0.092, 1.550±0.119, and 2.698±0.278, respectively, and there was statistical difference on the whole(F=638.245,P<0.001). The expression levels of miR-519d between each group were statistically different(PE group vs control group, T=18.226, P<0.001; severe PE group vs control group, T=26.588, P<0.001; PE group vs severe PE 山 东 大 学 学 报 (医 学 版)58卷1期 -张炜悦,等.microRNA519D与基质金属蛋白酶2在36例子痫前期患者胎盘组织中的表达及意义 \=- group, T=16.636, P<0.001). The expression levels of MMP2 in placental tissues of control group, PE group and severe PE group were 0.844±0.047, 0.458±0.039, and 0.273±0.012, respectively, and there was statistical difference on the whole (F=1 552.203,P<0.001). The expression levels of miR-519d between each group were statistically different(PE group vs control group, T=28.823, P<0.001; severe PE group vs control group, T=68.189, P<0.001; PE group vs severe PE group, T=18.442, P<0.001). MMP2 and miR-519d expressions exhibited a negative correlation(r=-0.907, P<0.001). Conclusion The expression of miR-519d in placental tissue of PE patients is increased, while the expression of MMP2 is decreased with the exacerbation of PE. MMP2 and miR-519d may participate in the pathogenesis of PE.

Key words: Pre-eclampsia, Placenca, Matrix metalloproteinase2, microRNA519D

CLC Number: 

  • R714.24
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