Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (12): 77-85.doi: 10.6040/j.issn.1671-7554.0.2020.0110

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Correlations of microRNA-103 and microRNA-107 expressions with the clinical characteristics and prognosis of 120 cases of sepsis

YANG Zhen1, ZHANG Yanmin1, WANG Qianqian1, CHEN Huimin1, FENG Qiang2, ZHOU Shaoying1   

  1. 1. Emergency Department, Handan Central Hospital, Handan 056001, Hebei, China;
    2. Department of Cardiology, Handan Central Hospital, Handan 056001, Hebei, China
  • Published:2020-12-08

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Abstract: Objective To evaluate the predictive values of plasma microRNA(miR)-103 and miR-107 for septic death, and their correlations with disease severity as well as prognosis. Methods A total of 120 sepsis patients who received treatment in our hospital during Jan. 2016 and Jun. 2019(sepsis group)were enrolled and their plasma samples were collected within 24 h after enrollment; meanwhile, 120 contemporaneous healthy controls(HC group)were recruited and their plasma samples were collected. Plasma miR-103 and miR-107 expressions were detected with reverse transcription-quantitative polymerase chain reaction. Several plasma inflammatory cytokines were detected with enzyme-linked immune sorbent assay. In sepsis patients, clinical characteristics were collected and 28-day mortality rate was calculated. The supportive diagnostic value for sepsis was analyzed with receiver operating characteristic(ROC)curve. The associated factors of sepsis death were analyzed with multivariate Coxs regression model. Results The sepsis group had reduced miR-103 and miR-107 expressions than HC group. The miR-103 and miR-107 expressions could serve as supportive indexes for sepsis diagnosis, and their area under curve(AUC)was 0.893(95%CI: 0.854-0.933)and 0.941(95%CI: 0.913-0.968), respectively. In sepsis group, miR-103 and miR-107 expressions were negatively correlated with acute pathologic and chronic health evaluation II score, sequential organ failure assessment score, serum creatinine, C-reactive protein, tumor necrosis factor-α, interleukin-1β, interleukin-6 and interleukin-8, while positively correlated with albumin. Moreover, miR-103 and miR-107 expressions decreased in septic deaths compared to septic survivors. Multivariate Coxs regression analysis revealed that miR-103 expression was an independent factor for 28-day mortality risk of sepsis patients. Conclusion Reduced miR-103 and miR-107 expressions are correlated with sepsis incidence, as well as disease severity and 28-day mortality.

Key words: microRNA-103, microRNA-107, Sepsis, Disease severity, Inflammation, 28-day mortality

CLC Number: 

  • R459.7
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