Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (12): 86-91.doi: 10.6040/j.issn.1671-7554.0.2020.0906

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Expression and function of miR-25-3p in endometrial cancer

SHI Li, MA Jing, ZHAO Xiwa, GUAN Yingxia, ZHAO Lianmei, SHAN Baoen   

  1. Department of Gynaecology and Obstetrics, Fourth Hospital of Hebei Medical University, Shijiazhuan 050011, Hebei, China
  • Published:2020-12-08

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Abstract: Objective To investigate the expression of miR-25-3p in endometrial cancer tissues, and to explore its effects on the proliferation, migration, invasion and apoptosis of KLE cells. Methods A total of 40 patients with endometrial cancer treated during Jan. and Dec. 2018 were enrolled. The cancer tissues and adjacent tissues were collected, and the miR-25-3p expressions were detected with Real-time PCR. After KLE cells were transfected with miR-25-3p mimics, they were divided into overexpression group and control group, whose miR-25-3p expressions were detected with Real-time PCR. The proliferation, migration, invasion and apoptosis of the two groups of KLE cells were detected with flow cytometry, Transwell assay, matrigel invasion assay, and Annexin v-PI double staining assay, respectively. Results The expression of miR-25-3p was 0.38±0.91 in cancer tissues and 0.17±0.51 in the adjacent tissues, and the difference was statistically significant (t=12.28, P=0.003). Flow cytometry showed the proliferation index was(59.41±0.78)% in the overexpression group and(40.69±0.78)% in the control group, and the difference was statistically significant(G0G1 phase: t=12.504, P<0.001; S phase: t=6.902, P=0.020; G2M phase: t=4.203, P=0.014; PI phase: t=12.475, P<0.001). Transwell assay showed the number of migration cells was 28.54±1.73 in the overexpression group and 19.21±1.62 in the control group, and the difference was statistically significant(t=8.404, P=0.001). Matrigel invasion assay showed the number of invasion cells was 36.66±1.53 in the overexpression group and 17.66±1.53 in the control group, and the difference was statistically significant(t=15.234, P<0.001). Annexin v-PI double staining assay showed the apoptosis rate was(2.75±0.58)% in the overexpression group and(4.81±0.31)% in the control group, and the difference was statistically significant(t=5.443, P=0.006). Conclusion miR-25-3p is highly expressed in endometrial cancer tissues. Overexpression of miR-25-3p promotes the proliferation, migration and invasion but inhibits the apoptosis of KLE cells.

Key words: miR-25-3p, Endometrial cancer, Proliferation, Migration, Invasion, Apoptosis

CLC Number: 

  • R737.33
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