Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (12): 77-85.doi: 10.6040/j.issn.1671-7554.0.2020.0110

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Correlations of microRNA-103 and microRNA-107 expressions with the clinical characteristics and prognosis of 120 cases of sepsis

YANG Zhen1, ZHANG Yanmin1, WANG Qianqian1, CHEN Huimin1, FENG Qiang2, ZHOU Shaoying1   

  1. 1. Emergency Department, Handan Central Hospital, Handan 056001, Hebei, China;
    2. Department of Cardiology, Handan Central Hospital, Handan 056001, Hebei, China
  • Published:2020-12-08

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Abstract: Objective To evaluate the predictive values of plasma microRNA(miR)-103 and miR-107 for septic death, and their correlations with disease severity as well as prognosis. Methods A total of 120 sepsis patients who received treatment in our hospital during Jan. 2016 and Jun. 2019(sepsis group)were enrolled and their plasma samples were collected within 24 h after enrollment; meanwhile, 120 contemporaneous healthy controls(HC group)were recruited and their plasma samples were collected. Plasma miR-103 and miR-107 expressions were detected with reverse transcription-quantitative polymerase chain reaction. Several plasma inflammatory cytokines were detected with enzyme-linked immune sorbent assay. In sepsis patients, clinical characteristics were collected and 28-day mortality rate was calculated. The supportive diagnostic value for sepsis was analyzed with receiver operating characteristic(ROC)curve. The associated factors of sepsis death were analyzed with multivariate Coxs regression model. Results The sepsis group had reduced miR-103 and miR-107 expressions than HC group. The miR-103 and miR-107 expressions could serve as supportive indexes for sepsis diagnosis, and their area under curve(AUC)was 0.893(95%CI: 0.854-0.933)and 0.941(95%CI: 0.913-0.968), respectively. In sepsis group, miR-103 and miR-107 expressions were negatively correlated with acute pathologic and chronic health evaluation II score, sequential organ failure assessment score, serum creatinine, C-reactive protein, tumor necrosis factor-α, interleukin-1β, interleukin-6 and interleukin-8, while positively correlated with albumin. Moreover, miR-103 and miR-107 expressions decreased in septic deaths compared to septic survivors. Multivariate Coxs regression analysis revealed that miR-103 expression was an independent factor for 28-day mortality risk of sepsis patients. Conclusion Reduced miR-103 and miR-107 expressions are correlated with sepsis incidence, as well as disease severity and 28-day mortality.

Key words: microRNA-103, microRNA-107, Sepsis, Disease severity, Inflammation, 28-day mortality

CLC Number: 

  • R459.7
[1] Singer M, Deutschman CS, Seymour CW, et al. The third international consensus definitions for sepsis and septic shock(sepsis-3)[J]. JAMA, 2016, 315(8): 801-810.
[2] Tiru B, DiNino EK, Orenstein A, et al. The economic and humanistic burden of severe sepsis [J]. Pharmacoeconomics, 2015, 33(9): 925-937.
[3] Howell MD, Davis AM. Management of sepsis and septic shock [J]. JAMA, 2017, 317(8): 847-848.
[4] Verdonk F, Blet A, Mebazaa A. The new sepsis definition: limitations and contribution to research and diagnosis of sepsis [J]. Curr Opin Anaesthesiol, 2017, 30(2): 200-204.
[5] Finnerty JR, Wang WX, Hebert SS, et al. The miR-15/107 group of microRNA genes: evolutionary biology, cellular functions, and roles in human diseases [J]. J Mol Biol, 2010, 402(3): 491-509.
[6] Wilfred BR, Wang WX, Nelson PT. Energizing miRNA research: a review of the role of miRNAs in lipid metabolism, with a prediction that miR-103/107 regulates human metabolic pathways [J]. Mol Genet Metab, 2007, 91(3): 209-217.
[7] Zhang W, Deng W, Wang Y. microRNA-103 promotes LPS-induced inflammatory injury by targeting c-Myc in HK-2 cells [J]. Artif Cells Nanomed Biotechnol, 2019, 47(1): 2791-2799.
[8] Hsieh CH, Rau CS, Jeng JC, et al. Whole blood-derived microRNA signatures in mice exposed to lipopolysaccharides [J]. J Biomed Sci, 2012, 19(1): 69.
[9] Gao ZF, Ji XL, Gu J, et al. microRNA-107 protects against inflammation and endoplasmic reticulum stress of vascular endothelial cells via KRT1-dependent Notch signaling pathway in a mouse model of coronary atherosclerosis [J]. J Cell Physiol, 2019, 234(7): 12029-12041.
[10] Lu Q, Ma Z, Ding Y, et al. Circulating miR-103a-3p contributes to angiotensin II-induced renal inflammation and fibrosis via a SNRK/NF-kappaB/p65 regulatory axis [J]. Nat Commun, 2019, 10(1): 2145.
[11] Wang S, Zhang Z, Wang J, et al. MiR-107 induces TNF-alpha secretion in endothelial cells causing tubular cell injury in patients with septic acute kidney injury [J]. Biochem Biophys Res Commun, 2017, 483(1): 45-51.
[12] Wang JX, Zhang XJ, Li Q, et al. MicroRNA-103/107 regulate programmed necrosis and myocardial ischemia/reperfusion injury through targeting FADD [J]. Circ Res, 2015, 117(4): 352-363.
[13] Marialbert AH, Fabian LD, Maria PY, et al. Lung transcriptomics during protective ventilatory support in sepsis-induced acute lung injury [J]. PLoS One, 2015, 10(7): e0132296.
[14] Dellinger RP, Levy MM, Rhodes A, et al. Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock, 2012 [J]. Intensive Care Med, 2013, 39(2): 165-228.
[15] Knaus WA, Zimmerman JE, Wagner DP, et al. APACHE-acute physiology and chronic health evaluation: a physiologically based classification system [J]. Crit Care Med, 1981, 9(8): 591-597.
[16] Vincent JL, Moreno R, Takala J, et al. The SOFA(sepsis-related organ failure assessment)score to describe organ dysfunction/failure. On behalf of the working group on sepsis-related problems of the european society of intensive care medicine [J]. Intensive Care Med, 1996, 22(7): 707-710.
[17] 章春霞, 廉姜芳. MiR-103功能的研究进展[J]. 生命的化学, 2018, 38(1): 104-108. ZHANG Chunxia, LIAN Jiangfang. Research progress on the roles of miR-103[J]. Chemistry of life,2018, 38(1): 104-108.
[18] Li G, Chen T, Zhu Y, et al. MiR-103 alleviates autophagy and apoptosis by regulating SOX2 in LPS-injured PC12 cells and SCI rats [J]. Iran J Basic Med Sci, 2018, 21(3): 292-300.
[19] 师灵灵, 韩艳秋, 任慧娟, 等. 脓毒症的病理生理机制研究进展 [J]. 中华医院感染学杂志, 2016, 26(8): 1914-1916. SHI Lingling, HAN Yanqiu, REN Huijuan, et al. Research advance of pathology and physiology of sepsis[J]. Chinese Journal of Nosocomiology, 2016, 26(8): 1914-1916.
[20] 曾小娜, 尹连红, 许丽娜. 脓毒症性急性肾损伤发病机制[J]. 生理科学进展, 2020, 51(2): 44-48.
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