Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (6): 19-25.doi: 10.6040/j.issn.1671-7554.0.2021.1348

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Role of NOX4 in ROS induction by Helicobacter pylori in gastric cancer cells

BAO Shuqing, YANG Mingyue, LIU Duanrui, WANG Yunshan, JIA Yanfei   

  1. Basic Medical Research Center, Central Hospital to Shandong First Medical University, Jinan 250013, Shandong, China
  • Published:2022-06-17

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Abstract: Objective To investigate the effect of nicotinamide adenine dinucleotide phosphate oxidase 4(NOX4)on reactive oxygen species(ROS)induced by Helicobacter pylori(H. pylori)in gastric cancer cells. Methods Gastric cancer cell line MKN-45 was co-cultured with H. pylori NCTC 26695 and NCTC 11637 for 6 hours respectively, the experiment included non-infection group and H. pylori infection group; MKN-45 cells were pretreated with 25mmol/L N-acetyl cysteine(NAC)for 30 minutes, NAC treated group and NAC untreated group were set up. The protein expression of NOX4 was detected with Western blotting. The level of ROS was detected with ROS detection kit. Changes in ROS level after H. pylori infection were observed with fluorescence microscope, and fluorescence intensity was detected with fluorescein microplate analyzer. Results ROS production increased in H. pylori infected group compared with non-infection group. NOX4 protein expression increased after NCTC 26695 infection(t=11.67, P<0.001), and increased after NCTC 11637 infection(t=17.29, P<0.001). After NAC pretreatment and H. pylori infection, NOX4 expression was significantly reduced ［NCTC 26695(P<0.001), NCTC 11637(P<0.001)］. Conclusion NOX4/ROS pathway may be involved in the activation of H. pylori redox signal in gastric cancer cells.

Key words: Reactive oxygen species, Nicotinamide adenine dinucleotide phosphate oxidase 4, N-acetylcysteine, Gastric cancer, Redox signal

CLC Number: 

  • R735.2
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