Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (7): 1-9.doi: 10.6040/j.issn.1671-7554.0.2021.0055

   

Expressions of DNA methyltransferases in gastric cancer and their clinical significance

CHU Yan1,2, LIU Duanrui1,2, ZHU Wenshuai1,2, FAN Rong1,2, MA Xiaoli1, WANG Yunshan1, JIA Yanfei1   

  1. 1. Research Center of Basic Medicine, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250013, Shandong, China;
    2. Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Published:2021-07-16

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Abstract: Objective To investigate the expressions of DNA methyltransferases(DNMT1, DNMT3A, DNMT3B)in gastric cancer and its correlation with the clinicopathological parameters and prognosis of gastric cancer. Methods Based on TCGA and GEO data, the differences in DNA methyltransferases expressions were compared between gastric cancer and adjacent normal tissues; the effects of DNA methyltransferases on the prognosis of patients with gastric cancer were analyzed with Kaplan-Meier plotter; the relationship between DNA methyltransferases and clinicopathological parameters was analyzed; the biological function of DNMT3B was explored with gene enrichment analysis(GSEA)binding protein interaction network. The mechanism of DNMT3B elevation in the progression of gastric cancer was also analyzed. Results The expressions of three DNA methyltransferases in gastric cancer tissues were higher than those in adjacent normal tissues; survival analysis showed that patients with high expressions of DNMT3A and DNMT3B had shorter overall survival; DNMT3B expression was related to the histological type, and was high in intestinal type gastric cancer; Helicobacter pylori infection promoted the expression of DNMT3B; DNMT3B promoted the progression of gastric cancer by affecting the methylation of BUB1 and HDAC2. Conclusion DNA methyltransferases are highly expressed in gastric cancer. DNMT3B is a prognostic indicator of gastric cancer and may be a biomarker for the clinical diagnosis and treatment of gastric cancer.

Key words: DNA methyltransferase, Gastric cancer, Prognosis, TCGA database, GEO database

CLC Number: 

  • R735.2
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