Table of Content

  

10 July 2021
Volume 59 Issue 7

Expressions of DNA methyltransferases in gastric cancer and their clinical significance

CHU Yan, LIU Duanrui, ZHU Wenshuai, FAN Rong, MA Xiaoli, WANG Yunshan, JIA Yanfei

Journal of Shandong University (Health Sciences). 2021, 59(7):  1-9.  doi:10.6040/j.issn.1671-7554.0.2021.0055

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Objective To investigate the expressions of DNA methyltransferases(DNMT1, DNMT3A, DNMT3B)in gastric cancer and its correlation with the clinicopathological parameters and prognosis of gastric cancer. Methods Based on TCGA and GEO data, the differences in DNA methyltransferases expressions were compared between gastric cancer and adjacent normal tissues; the effects of DNA methyltransferases on the prognosis of patients with gastric cancer were analyzed with Kaplan-Meier plotter; the relationship between DNA methyltransferases and clinicopathological parameters was analyzed; the biological function of DNMT3B was explored with gene enrichment analysis(GSEA)binding protein interaction network. The mechanism of DNMT3B elevation in the progression of gastric cancer was also analyzed. Results The expressions of three DNA methyltransferases in gastric cancer tissues were higher than those in adjacent normal tissues; survival analysis showed that patients with high expressions of DNMT3A and DNMT3B had shorter overall survival; DNMT3B expression was related to the histological type, and was high in intestinal type gastric cancer; Helicobacter pylori infection promoted the expression of DNMT3B; DNMT3B promoted the progression of gastric cancer by affecting the methylation of BUB1 and HDAC2. Conclusion DNA methyltransferases are highly expressed in gastric cancer. DNMT3B is a prognostic indicator of gastric cancer and may be a biomarker for the clinical diagnosis and treatment of gastric cancer.

Effects of calcitriol on EMT induced by TGF-β1 in asthma

ZHANG Qian, QIN Mingming, HE Xuejia, CAI Qiujing, ZHANG Yamin, LI Qingsu, ZHU Weiwei

Journal of Shandong University (Health Sciences). 2021, 59(7):  10-18.  doi:10.6040/j.issn.1671-7554.0.2021.0335

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Objective To investigate the effects of calcitriol on the epithelial-mesenchymal transformation(EMT)of human bronchial epithelial cells(BEAS-2B)induced by transforming growth factor-β1(TGF-β1), and to provide reference for the prevention and treatment of asthma airway remodeling. Methods The optimal time of TGF-β1 acting on BEAS-2B cells to induce EMT was screened, and the cells were divided into blank group, 24 h TGF-β1 group, 48h TGF-β1 group, and 72 h TGF-β1 group. The optimal concentration of TGF-β1 acting on BEAS-2B cells to induce EMT was screened, and the cells were divided into blank group, 0.1 ng/mL TGF-β1 group, 1 ng/mL TGF-β1 group, 10 ng/mL TGF-β1 group, and 100 ng/mL TGF-β1 group. After pretreatment with calcitriol, the cells were divided into blank group, TGF-β1 group, calcitriol group, and TGF-β1+calcitriol group. The expressions of E-Cadherin, N-Cadherin, p-Akt and p-mTOR were detected with Western blotting. The migration ability of cells was detected with Transwell assay and scratch test. Results Western blotting showed statistically significant differences in the expressions of E-Cadherin(F=53.245, P<0.001)and N-Cadherin(F=54.429, P<0.001)in the blank group, 24 h TGF-β1 group, 48 h TGF-β1 group and 72 h TGF-β1 group. The expression of E-Cadherin in the 1ng/mL TGF-β1 group, 10 ng/mL TGF-β1 group and 100 ng/mL TGF-β1 group were significantly different with that in the blank group(F=27.368, P<0.001), and the expression of N-Cadherin was also different(F=14.272, P<0.001), among which the 10ng/ml TGF-β1 group for 48h showed the most significant difference. TGF-β1 induced the expression of PI3K/Akt signaling pathway related proteins and the expressions of interstitial markers in human bronchial epithelial cells, and the effects of TGF-β1 were statistically significant(P<0.001). However, calcitriol attenuated the effects, and the effects of calcitriol were statistically significant(P<0.001). There was no interaction between TGF-β1 and calcitriol(P>0.05). The results of Transwell test and scratch test showed that the migration ability of BEAS-2B cells in TGF-β1 treatment group was increased compared with that in the blank group(P<0.001). Compared with the TGF-β1 group, the TGF-β1+calcitriol group had decreased migration ability(P<0.001). Conclusion Calcitriol can inhibit the EMT bronchial epithelial cells induced by TGF-β1, thereby reducing airway inflammation and airway remodeling in asthma, which may involve calcitriol reducing the TGF-β1-activated proteins of PI3K/Akt/mTOR signaling pathway.

Related factors of osteoporosis in 210 postmenopausal women with type 2 diabetes mellitus

LYU Li, JIANG Lu, CHEN Shihong, ZHUANG Xianghua, SONG Yuwen, WANG Dianhui, AN Wenjuan, LI Qian, PAN Zhe

Journal of Shandong University (Health Sciences). 2021, 59(7):  19-25.  doi:10.6040/j.issn.1671-7554.0.2021.0633

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Objective To investigate the related factors of osteoporosis(OP)in postmenopausal women with type 2 diabetes mellitus(T2DM)and to analyze their predictive value. Methods A total of 337 postmenopausal women with T2DM were selected. According to the results of bone mineral density, the patients were divided into the OP group(n=210)and normal bone mass group(NBM group, n=127). The general data of the subjects were recorded, and indexes such as HbA1c, liver function, kidney function and blood lipid were detected. The bone mineral density was measured with dual-energy X-ray absorptiometry. SPSS 22.0 software was used for statistical analysis. The general data of the two groups were statistically analyzed. Logistics regression was used for multivariate analysis. Pearson correlation analysis was used to analyze the correlation among menopausal duration, age of menarche, age of menopause and other indicators. Receiver operating characteristic(ROC)curve was drawn and the area under the curve(AUC)was calculated to evaluate the value of multivariate regression model in the diagnosis and prediction of OP. Results The age(P<0.001), duration of diabetes(P<0.001), age of menarche(P=0.001)and menopausal duration(P<0.001)in the OP group were higher or longer than those in NBM group, while the body mass index(BMI)in OP group was lower than that in NBM group(P=0.006). Binary Logistic regression results showed that age(OR=1.10, 95%CI=1.01-1.21, P=0.031), menopausal duration(OR=1.17, 95%CI=1.07-1.28, P<0.001), complicated nephropathy (OR=3.52, 95%CI=1.42-8.75, P=0.007)and calcium intake(OR=11.41, 95%CI=4.94-26.34, P<0.001)were risk factors for OP, while BMI(OR=0.86, 95%CI=0.78-0.95, P=0.002)and habitual exercise(OR=0.28, 95%CI=0.13-0.60, P<0.001)were protective factors. The AUC was 0.919; the optimal diagnostic cut-off value was 0.665; the sensitivity and specificity of diagnostic efficacy were 81.3% and 89.8%, respectively. Conclusion Old age, long menopausal duration, complicated nephropathy, low BMI and lack of habitual exercise are the risk factors for OP in postmenopausal women with T2DM. The association between calcium intake and increased risk of OP may be related to the intentional increase in calcium intake.

A retrospective analysis of chromosome karyotypes in RSA couples from assisted reproductive population

ZHAO Bingqing, GAO Xuan, LI Jiangxia

Journal of Shandong University (Health Sciences). 2021, 59(7):  26-31.  doi:10.6040/j.issn.1671-7554.0.2021.0102

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Objective To analyze the peripheral blood chromosome karyotypes of patients assisted with reproductive technology, so as to provide reference for the clinical diagnosis and treatment and genetic counseling. Methods From Jan. 2015 to Dec. 2018, 1 438(719 couples)patients with recurrent spontaneous abortion(RSA)and 3 292(1 646 couples)patients with one-time spontaneous abortion undergoing assisted reproduction were selected for chromosome karyotype analysis. The types, incidences and ratios of chromosome variations between males and females were compared. Relationship between the number of spontaneous abortions and chromosome karyotypes was analyzed. Results Of the 1 438(719 couples)RSA patients, 320 chromosome abnormalities were detected, among which 165 were male cases and 155 were female cases. Chromosomal structural aberrations accounted for 57.58% of chromosomal abnormalities. The ratio of mutual translocations was the highest. For the number of abortions, significant differences were detected in chromosome reciprocal translocation and Robertsonian translocation between one-time spontaneous abortion group and RSA group(P<0.01), but no significant difference in chromosome inversion was observed(male P=0.21, female P=0.83). As the number of abortions increased, the trends of male and female chromosome aberration were statistically significant(P<0.01). Conclusion The rate of chromosomal abnormality in patients with one-time spontaneous abortion is lower than that in RSA patients. The most common chromosomal abnormality in RSA patients is chromosomal translocation, and the rate of chromosomal abnormality is not related to the number of abortions.

Effect of Gn stimulation duration on pregnancy outcome of IVF-ET in long GnRHa protocol

WU Xiaolin, LI Ping

Journal of Shandong University (Health Sciences). 2021, 59(7):  32-37.  doi:10.6040/j.issn.1671-7554.0.2020.1671

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Objective To investigate the effect of the duration of gonadotropin(Gn)in the controlled ovarian hyper stimulation(COH)cycle on the number of eggs obtained, embryo development, and pregnancy outcome in those with good ovarian response, and to analyze the possible factors that contributed to the prolongation of Gn use. Methods A total of 3 054 patients undergoing in vitro fertilization-embryo transfer(IVF-ET)were divided into groups A, B, and C according to the days of Gn use(group A: Gn days≤7 d; group B: 8 d ≤Gn days ≤11 d; group C: Gn days ≥12 d).The baseline data, COH and pregnancy outcomes were compared. The possible factors leading to the prolongation of Gn use were analyzed with Logistics regression. Results The highest BMI and FSH levels were found in group C(P=0.001), while the lowest AMH and AFC levels were found in group C(P=0.022, 0.002). The Gn starting dose, total Gn dose and downregulation days in group A were the least among the three groups(P<0.001). There were no significant differences among the three groups in indicators related to COH treatment and pregnancy outcomes(P=0.86, 0.57, 0.91, 0.64). Logistics regression showed that higher BMI and Gn starting dose were risk factors of prolonged Gn use, while higher AMH and AFC, lower FSH level and shorter downregulation days were protective factors of prolonged Gn use. Conclusion The duration of Gn use has no significant effect on the pregnancy outcomes of IVF-ET. Higher BMI and Gn starting dose can significantly prolong Gn use, while higher AMH and AFC, lower FSH level and shorter downregulation days can significantly shorten Gn use.

Risk factors influencing prognosis of lower extremity deep vein thrombosis in pregnant and parturient women

CHEN Liyu, XIAO Juan, LYU Xianzhong, DUAN Baomin, HONG Fanzhen

Journal of Shandong University (Health Sciences). 2021, 59(7):  38-42.  doi:10.6040/j.issn.1671-7554.0.2021.0482

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Objective To investigate the risk factors affecting the prognosis of deep vein thrombosis(DVT)in pregnant and parturient women. Methods Clinical data of 37 pregnant women with DVT who underwent obstetric examination and delivered in The Second Hospital of Shandong University during Dec. 2015 and Jan. 2020 were retrospectively analyzed. According to thrombus status, the subjects were divided into two groups: thrombus disappearance group(n=13)and thrombus residue group(n=24). Independent sample t test, rank sum test(Mann-Whitney U test)and χ2 test were used for univariate analysis of clinical characteristics, laboratory indicators and VTE risk factors score of the two groups. Results The D-dimer was 1 340.50(659.00, 2 447.75)ng/mL in the thrombus disappearance group, and 3 105.00(1 709.50, 6 146.00)ng/mL in the thrombus reside group(P=0.009). VTE risk factors score was 3.31±1.11 in the thrombus disappearance group and 4.96±1.94 in the thrombus residue group(P=0.002). The number of cases complicated with preeclampsia during pregnancy was 0 case(0%)in the thrombus disappearance group and 6 cases(25%)in the thrombus residue group(P=0.038). Conclusion High score of VTE risk factors, complicated preeclampsia and high concentration of D-dimer are the risk factors for residual thrombus in pregnant and maternal DVT patients.

Machine learning models based on interpretive CMR parameters can predict the prognosis of pediatric myocarditis

TIAN Yaotian, WANG Bao, LI Yeqin, WANG Teng, TIAN Liwen, HAN Bo, WANG Cuiyan

Journal of Shandong University (Health Sciences). 2021, 59(7):  43-49.  doi:10.6040/j.issn.1671-7554.0.2021.0031

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Objective To develop and validate machine learning models based on interpretive cardiac magnetic resonance(CMR)parameters for prognosis evaluation of pediatric myocarditis. Methods A retrospective analysis of 45 pediatric patients with myocarditis was conducted. According to whether adverse cardiac events(ACE)occurred, the patients were divided into poor prognosis group(n=18)and good prognosis group(n=27). CMR scans were performed after hospitalization and 206 interpretive CMR parameters about myocardial function, myocardial strain, first-pass perfusion and late gadolinium enhancement(LGE)were obtained. The parameters were trained by the classification learner App in MATLAB and the training model with the highest accuracy was chosen as the best model. The receiver-operating characteristics(ROC)curve of the machine learning model was drawn to determine the prognostic performance. Results A total of 14 CMR parameters were selected as predictive factors, and those without correlation were used to construct the combined parameters. Among all these parameters, maximal signal intensity percentage(SI %)of the 7th segment of AHA had the best performance(AUC: 0.790, sensitivity: 0.667, specificity: 0.833). Combined parameters achieved the highest performance(AUC: 0.940, sensitivity: 0.750, specificity: 0.889). Conclusion The machine learning models based on interpretive CMR parameters can be used for prognosis evaluation of pediatric myocarditis, and combination of interpretive CMR parameters training with machine learning is more accurate than single ones.

Effects of bedside lung ultrasonography and lung ultrasonography score in the diagnosis and treatment of 70 cases of neonatal respiratory distress syndrome

LIU Xiao, GUO Xinyuan, ZHANG Dejian, LI Qi, LI Ning, XUE Jiang

Journal of Shandong University (Health Sciences). 2021, 59(7):  50-56.  doi:10.6040/j.issn.1671-7554.0.2020.1715

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Objective To investigate the effects of bedside lung ultrasonography(LUS)and LUS scores on diagnosing neonatal respiratory distress syndrome(NRDS)and evaluating the pharmacological effects of pulmonary surfactant(PS). Methods A total of 70 neonates who were diagnosed with NRDS were allocated to the observation group, while the other 70 without lung diseases were allocated to the control group. The chest surface reflection was divided to 12 zones. NRDS signs in LUS and LUS scores were recorded. Bedside LUS was performed before PS treatment in the observation group, while LUS was performed after enrollment in the control group. The LUS results between two groups were compared to evaluate the diagnostic value of LUS in NRDS neonates. A total of 60 newborns in the observation group who need PS underwent another bedside LUS examination at 12 h after PS application. NRDS signs in LUS and LUS scores were compared before and after PS application, and the blood gas analysis and chest X-ray results were also compared. The role of LUS in the therapeutic effect of PS was also evaluated. Results The detection rate of NRDS signs in LUS and LUS score in observation group were significantly higher than those in control group(P<0.001). LUS score showed a high sensitivity(98.57%)and specificity(100%)in the diagnosis of NRDS, and it predicted the use of PS in neonates with NRDS with a high sensitivity(90.00%)and specificity(90.00%). NRDS signs in the observation group reduced significantly 12 h after PS application than before, and LUS scores also decreased(P<0.001). Conclusion Bedside LUS is accurate and reliable in diagnosis of NRDS. The effects of PS can be accurately evaluated by real-time bedside LUS and LUS scores.

Value of double-phase enhancement CT scan in the differential diagnosis of 182 benign and malignant thyroid nodules

XIA Xiaona, HUANG Zhaodi, REN Qingguo, LIU Feng, DENG He, REN Guorong, DUAN Jiandong, WANG Shaoyu

Journal of Shandong University (Health Sciences). 2021, 59(7):  57-62.  doi:10.6040/j.issn.1671-7554.0.2021.0266

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Objective To evaluate the value of double-phase enhancement CT scan in the differential diagnosis of benign and malignant thyroid nodules. Methods The CT data of 182 nodules confirmed by histology in 175 patients were retrospectively analyzed, including 60 benign nodules and 122 malignant nodules. The shape, density, location, CT difference between thyroid and nodules(ΔCT)and reinforcement type were evaluated between the benign and malignant groups. Univariate and multivariate dichotomous Logistic regression were performed to evaluate the OR value of CT features of benign and malignant nodules, and the area under the receiver operating characteristic(ROC)curve were compared between the two groups. Results There were significant differences in the shape(χ2=50.661, P<0.001), density(χ2=60.042, P<0.001), location(χ2=48.916, P<0.001)and ΔCT value(χ2=45.647, P<0.001)between benign and malignant nodules, but there was no significant difference in reinforcement type(χ2=1.586, P=0.434). The area under the ROC curve was 0.899 for the combined prediction probability of four positive signs. When the prediction probability was 0.711, the Youden Index reached its maximum value, and the sensitivity and specificity of diagnosing malignant nodules were 0.869 and 0.783, respectively. Conclusion CT double-phase enhanced scan is of great value in the differential diagnosis of benign and malignant thyroid nodules. Combination of multiple CT signs can improve the accuracy of diagnosis.

Value of regular ultrasound examination in the diagnosis and treatment of deep venous thrombosis associated with central venous access device

WANG Ning, GUO Zhenjiang, ZHANG Yuanyuan, WANG Jing, GUO Wei, WANG Jinrong, CUI Zhaobo

Journal of Shandong University (Health Sciences). 2021, 59(7):  63-67.  doi:10.6040/j.issn.1671-7554.0.2020.1717

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Objective To evaluate the value of regular ultrasound examination in the diagnosis and treatment of deep vein thrombosis associated with central venous access device(CVAD). Methods A total of 440 cancer patients with CAVD treated in Harrsion International Peace Hospital during May 2018 and Sep. 2019 were randomly divided into the observation group(n=221)and observation group(n=219). The observation group received ultrasound examination every 3 weeks to detect the presence of CVAD-related deep venous thrombosis(CRDVT), while the control group received ultrasound examination when clinical symptoms appeared. The patients with CRDVT diagnosed in the two groups were evaluated with ultrasound for no less than 90 days until CVAD was removed, and the thrombosis recurrence during this period was recorded. The diagnostic rate of CRDVT, rate of unplanned CVAD removal, rate of thrombosis recurrence and incidence of post thrombotic syndrome(PTS)were compared between the two groups. Results There was no significant difference in the basic data between the two groups(P>0.05). The diagnostic rate of CRDVT was 9.5%(21 cases)in the observation group and 4.6%(10 cases)in the control group(χ2=4.092, P=0.043). The rate of unplanned CVAD removal was 0.9%(2 cases)in the observation group and 4.1%(9 cases)in the control group(χ2=4.634, P=0.031). The cumulative thrombosis recurrence rate of CRDVT patients in 90 days was 15.3% in the observation group and 10.0% in the control group(Logrank=0.003, P=0.953). The incidence of PTS was 4.7% in the observation group and 40% in the control group(χ2=3.886, P=0.049). Conclusion Regular ultrasonic examination can improve the diagnostic rate of CRDVT and reduce the rate of unplanned CVAD removal and PTS incidence.

Diagnostic value of contrast-enhanced endoscopic ultrasonography combined with endoscopic ultrasound-guided fine needle aspiration for pancreatic masses

CHEN Gang, ZHANG Yanping, YE Leping, FU Chong, ZHOU Chunyan, ZHANG Shidong

Journal of Shandong University (Health Sciences). 2021, 59(7):  68-73.  doi:10.6040/j.issn.1671-7554.0.2021.0038

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Objective To explore the diagnostic value of contrast-enhanced endoscopic ultrasonography(CE-EUS)combined with endoscopic ultrasound-guided fine needle aspiration(EUS-FNA)for pancreatic cancer. Methods The clinical data of 175 patients with pancreatic space-occupying lesions treated during Jan. 2017 and Dec. 2020 were retrospectively analyzed. Based on the results of surgery and puncture pathology or follow-up data, 132 patients met the inclusion requirements, including 55 patients who received enhanced CT and/or enhanced MRI, 40 patients who received EUS examination, and 37 patients who received CE-EUS combined with EUS-FNA. The value of the three methods in the diagnosis of pancreatic masses were compared. Results The results of enhanced CT and/or enhanced MRI showed 13 benign cases and 42 malignant cases. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 79.49%(31/39), 56.25%(9/16), 81.58%(31/38), 52.94%(9/17), and 72.73%(40/55), respectively. EUS showed 13 benign cases and 27 malignant cases. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 80.00%(24/30), 60.00%(6/10), 85.71%(24/28), 50.00%(6/12), and 75.00%(30/40), respectively. CE-EUS combined with EUS-FNA showed 14 benign cases and 23 malignant cases. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 93.94%(31/33), 75.00%(3/4), 96.87%(31/32), 60.00%(3/5), and 91.89%(34/37), respectively. Pancreatic masses showed uneven spots or rod-like low enhancement mode under CE-EUS, and FNA in the low enhancement area could improve the positive rate of puncture. Conclusion CE-EUS combined with EUS-FNA can improve the diagnostic accuracy of pancreatic masses, which is worthy of clinical promotion.

Construct of a risk assessment model of breast cancer immune-related lncRNAs based on the database information

LI Wanwan, ZHOU Wenkai, DONG Shuqing, HE Shiqing, LIU Zhao, ZHANG Jiaxin, LIU Bin

Journal of Shandong University (Health Sciences). 2021, 59(7):  74-84.  doi:10.6040/j.issn.1671-7554.0.2021.0002

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Objective To screen the immune-related long non-coding RNA(lncRNA)in breast cancer, construct a breast cancer prognostic risk assessment model, and explore the prognostic factors. Methods The sequencing data, clinical information and immune gene set of breast cancer patients from the official websites of UCSC Xena(https://xena.ucsc.edu/), TCGA, and immport(https://www.immport.org/home)were downloaded. The data were sorted and cleaned, and finally breast cancer immune-related lncRNA expression matrix and clinical information were got. Univariate Cox regression analysis and Multivariate Cox regression analysis were used to screen out lncRNAs related to prognosis, so as to construct a prognostic risk score. According to the median risk score, the patients were divided into high-risk and low-risk groups. Kaplan-Meier(K-M)survival analysis, receiver operating characteristic(ROC)curve analysis and independent prognostic factor evaluation were used to evaluate the model. Combined with other clinical factors and risk scores of breast cancer, a nomogram was drawn to predict the individual survival rate of breast cancer patients. Results Ten immune-related lncRNAs were determined to construct a risk scoring model. The high-risk group had a poorer prognosis compared with the low-risk group. Risk score could be used as an independent prognostic factor for breast cancer. The C-index(CI)of the nomogram was 0.751. The calibration chart showed that the predicted value was in good agreement with the actual observation value. Conclusion A risk scoring model composed of 10 immune-related lncRNAs can be used to assess the prognosis of the breast cancer patients, and the corresponding nomogram can further predict the survival rate.

Reliability and validity of Co-rumination Questionnaire in 1 354 college nursing students

ZHANG Yunxue, XIE Zihui, LYU Gaorong, SHEN Shiyu, LI Ping

Journal of Shandong University (Health Sciences). 2021, 59(7):  85-90.  doi:10.6040/j.issn.1671-7554.0.2021.0039

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Objective To assess co-rumination of college nursing students, translate the English version of Co-rumination Questionnaire(CRQ)into Chinese and revise it, and evaluate the reliability and validity of the scale. Methods After translating the English version of CRQ with the permission of the author of the original English scale, totally 1 354 nursing students completed the Chinese version of CRQ for investigating co-rumination in which individuals excessively discussed personal problems within a dyadic relationship. Then the reliability and validity of the Chinese version of CRQ were tested. Results The simplified Chinese version of 9-item Co-rumination Questionnaire(CRQ-9)was obtained. The results of confirmatory factor analysis showed that the fitting degree of the single-dimensional model was good, with χ2/df and root mean square error of approximation(RMSEA)being 2.584 and 0.048, respectively, and goodness-of-fit index(GFI), adjusted goodness-of-fit index(AGFI), comparative fit index(CFI), incremental fit index(IFI), and Tacker-Lewis index(TLI)being more than 0.900. The score of CRQ-9 was positively correlated with anxiety, depression, perspective taking and personal distress(r=0.195-0.315, all P<0.001). Cronbach α coefficient was 0.875 and Guttman split-half reliability was 0. 899(P<0.01). Conclusion The single factor structure of CRQ-9 is consistent with the original scale and CRQ-9 has good reliability and validity, and can be generalized to evaluate co-rumination of Chinese college nursing students.

Correlation among personality traits and depression, anxiety symptoms in patients after colorectal cancer resection

SUN Fuyun, WANG Weipeng, ZHANG Huihui, GENG Yan, AN Xiaoxia, LI Shuangshuang, ZHANG Binbin

Journal of Shandong University (Health Sciences). 2021, 59(7):  91-96.  doi:10.6040/j.issn.1671-7554.0.2021.0168

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Objective To explore the correlation among personality traits and depression, anxiety symptoms in patients after colorectal cancer resection. Methods Totally 162 patients with colorectal cancer hospitalized in Department of General Surgery, Qilu Hospital of Shandong University from Feb. to Dec. 2018 were assessed before discharge with General Condition Questionnaire, Hospital Anxiety and Depression Scale, Eysenck Personality Questionnaire, and Lifestyle Self-Rating Scale, to measure the general condition, depression and anxiety symptoms, extraversion, neuroticism, psychoticism, personality stability, and health condition of living style. Data collected were analyzed with multiple linear regression. Results Depression and anxiety symptoms were reported by 37.0% and 40.2% patients, respectively. Multiple linear regression suggested that extraversion was negatively correlated with depression(β=-0.492, P<0.001)and anxiety symptoms(β=-0.336, P<0.001), while neuroticism was positively correlated with depression(β=0.166, P=0.016)and anxiety symptoms(β=0.345, P<0.001). Conclusion The positive rates of the depression and anxiety symptoms are high in patients after colorectal cancer resection. Extroversion is negatively correlated with depression and anxiety symptoms, while neuroticism is positively correlated with depression and anxiety symptoms.

Impact of social capital on health promotion behaviors of the elderly

WANG Xin, GUO Dan, LIANG Jiarui, GUO Li, WANG Jian

Journal of Shandong University (Health Sciences). 2021, 59(7):  97-103.  doi:10.6040/j.issn.1671-7554.0.2021.0023

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Objective To Explore the influence of social capital on the health promotion behavior of the elderly, and provide scientific basis for improving the health promotion behavior level of the elderly and improving their health status. Methods In March 2019, through a multi-stage stratified random sampling method, using self-designed general situation questionnaires, social capital questionnaires, and the revised Chinese version of the Health Promotion Lifestyle Rating Scale(HPLP-IIR), a questionnaire survey was conducted among 356 community elderly over 60 years old in Taiyuan City, Shanxi Province. The data were analyzed by t-test, analysis of variance, Pearson correlation and multiple stepwise regression methods. Results The health promotion lifestyle score of the elderly in Taiyuan community was 108.84±33.43, which was in a good level. The differences in health promotion behaviors of the elderly with different gender, income status and education level were statistically significant(all P<0.05). Pearson correlation analysis showed that the social capital and its dimensions of the elderly were positively correlated with health promotion behaviors and its dimensions(r=0.102-0.424, all P<0.05). The results of multiple stepwise regression analysis showed that gender(β=0.107), income status(β=0.194), social connection(β=0.319), and social control(β=0.175)were influencing factors of health-promoting behaviors, accounting for 23.2% of the variance in health-promoting behaviors. Conclusion By increasing the level of social capital of the elderly, their health promotion behaviors can be significantly improved. Among them, social connection and social control show a strong positive effect.

Causal influence of lung function on risk of fatality of COVID-19: a two-sample Mendelian randomization study

YANG Xuan, LI Yanzhi, MA Wei, JIA Chongqi

Journal of Shandong University (Health Sciences). 2021, 59(7):  104-111.  doi:10.6040/j.issn.1671-7554.0.2021.0241

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Objective To investigate whether lung function was causally associated with risk of fatality of COVID-19 based on a two-sample Mendelian randomization study. Methods This two-sample Mendelian randomization study used summary-level datasets of genome-wide association studies(GWAS)of forced vital capacity(FVC)and risk of fatality of COVID-19 from the UK Biobank. Inverse-variance weighted(IVW), MR-Egger regression, and weighted median estimator(WME)were conducted to investigate the association of lung function with risk of fatality of COVID-19, in which the OR values were used as indicators. Results A total of 287 single nucleotide polymorphisms were enrolled as instrumental variables. Statistically significant directional pleiotropy was not found(P=0.107). IVW regression demonstrated that per 1 elevated SD of FVC resulted in decreasing 62% of fatality risk in COVID-19 patients(OR=0.38, 95%CI: 0.18-0.80). MR-Egger regression also obtained a similar effect(OR=0.08, 95%CI: 0.01-0.61). WME analysis showed no significant association of FVC with risk of fatality of COVID-19(OR=0.44, 95%CI: 0.14-1.42). Conclusion Lung function might be negatively related to risk of fatality of COVID-19.

Application of ARIMA multiplicative seasonal model in the prediction of pulmonary tuberculosis incidence in Shandong Province

TIAN Qing, LIU Yongpeng, ZHANG Jingjing, LIU Hongqing

Journal of Shandong University (Health Sciences). 2021, 59(7):  112-118.  doi:10.6040/j.issn.1671-7554.0.2021.0049

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Objective According to the seasonality and trend of pulmonary tuberculosis in Shandong Province, to establish an Autoregressive Integrated Moving Average(ARIMA)product seasonal model, predict the incidence of pulmonary tuberculosis in Shandong Province and adjust prevention and control measures. Methods An optimal model for the monthly data of pulmonary tuberculosis infectious diseases in Shandong Province from January 2010 to December 2019 was established using R software. The number of pulmonary tuberculosis cases from January to October 2020 was predicted and compared with the actual value to evaluate the prediction effect of the model. Further, the incidence trend of pulmonary tuberculosis cases from November 2020 to December 2021 was predicted. Results The number of pulmonary tuberculosis cases in Shandong Province showed an annual cycle, and the optimal model is ARIMA(3,1,0)(0,1,1)₁₂. The accuracy of fitting results from January 2010 to December 2019 showed that the mean absolute percentage error was only 5.50%. The mean absolute percentage error of the model prediction from January to October 2020 was 21.69%. Compared with the same period, there was a slight change in the number of cases from November 2020 to December 2021. Conclusion The ARIMA product seasonal model can rather satisfactorily fit and predict the incidence of tuberculosis in Shandong Province.

Comprehensive teaching reform and exploration of Medical Statistics under the guidance of “student-centered” concept

LIU Jing, YUAN Zhongshang, LI Xiujun, SUN Xiubin, MA Xufei, ZHAO Xiulan

Journal of Shandong University (Health Sciences). 2021, 59(7):  119-124.  doi:10.6040/j.issn.1671-7554.0.2021.0216

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Objective To carry out comprehensive teaching reform and practice in view of the widespread anxiety and negative attitudes among medical students in studying Medical Statistics in order to improve the teaching effects of this course. Methods Teachers enhanced their level of educational theories. Adhering to moral education value orientation and based on “student-centered” idea and the cognitive law of “practice-theory-practice”, the team carried out empirical research on students psychological problems in learning, helped students overcome learning barriers, modulated teaching practice with the research results, and then conducted out a comprehensive teaching reform of “idea promotion, reform practice and effect evaluation” on the course of Medical Statistics. Results Teachers theoretical level and teaching skills were improved, and students interests in learning and innovation were enhanced. Students attitudes towards this course became positive, their anxiety was alleviated, and their course performance was significantly improved. As a result, the teaching objectives were effectively achieved. Conclusion This reform is a successful attempt to implement the “student-centered” concept into teaching practice, which provides valuable experience and a new direction for the teaching reform of Medical Statistics.