Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (2): 34-40.doi: 10.6040/j.issn.1671-7554.0.2020.1588

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Expression and clinical significance of circulating CD56bright subset of NK cells in patients with Parkinsons disease

LI Xiangqing, YIN Xin, ZHAO Xuelian, ZHAO Peiqing   

  1. Department of Neurology, Zibo Central Hospital, Zibo 255036, Shandong, China
  • Published:2021-03-05

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Abstract: Objective To analyze the expression and clinical significance of circulating CD56bright subset of NK cellsinpatients with Parkinsons disease(PD). Methods A total of 56 PD patients treated during Oct. 2017 and Dec. 2019 and 50 healthy controls matched in age, sex and living environment were enrolled. The levels of cytokines in the peripheral blood of PD patients were detected with ELISA; the ratios of NK cells and CD56bright subset were analyzed with flow cytometry; PD patients were stratified with Hoehn-Yahr staging scale and unified Parkinsons disease rating scale(UPDRS); the correlation between the ratio of CD56bright subset and disease progression was analyzed. Finally, CD56bright subset was selected and cultured to clarify the differences in cytokines between PD group and control group. Results The levels of cytokines in PD group were significantly higher than those in the control group［IL-1β(t=2.46, P=0.015 4), IL-6(t=3.19, P=0.001 9), TNF-α(t=3.37, P=0.001 1), IFN-γ(t=6.36, P<0.001)］. The ratio of NK cells(t=3.13, P=0.007 8)and CD56bright subset(t=4.23, P<0.001)in PD group were significantly increased. The ratio of CD56bright subset was positively correlated with the progression of PD［Hoehn-Yahr staging scale(t=3.79, P=0.005 2), UPDRS(t=3.08, P=0.041)］. PD group could secrete more cytokines than control group after LPS stimulation［IL-1β(t=2.89, P=0.044 6), IL-6(t=3.15, P=0.034 7),TNF-α(t=4.59, P=0.010 1), IFN-γ(t=4.79, P=0.008 7)］. Conclusion NK cells and CD56bright subset are highly expressed in PD patients. CD56bright subset can promote the progression of PD by secreting cytokines, and CD56bright subset can become a new biomarker for diagnosis and a new target for predicting PD prognosis.

Key words: Parkinsons disease, NK cells, CD56bright subset, Disease prognosis, Cytokines

CLC Number: 

  • R741.04
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