Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (5): 43-49.doi: 10.6040/j.issn.1671-7554.0.2022.0121

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Characterization of regulatory T cells in gastric cancer based on single-cell RNA sequencing data

GAO Huiru1,2, DU Tiantian1,2, WANG Yunshan1,2, DU Lutao1,2, WANG Chuanxin1,2   

  1. 1. Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, Shandong, China;
    2. Tumor Marker Detection Engineering Laboratory of Shandong Province, Jinan 250033, Shandong, China
  • Published:2022-06-01

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Abstract: Objective To explore the cell composition of gastric cancer and normal gastric tissues and to analyze the difference in regulatory T cells between gastric cancer and normal gastric tissues. Methods The downloaded single-cell RNA sequencing data were subjected to quality control, dimensional-reduction clustering and cell annotation. Regulatory T cells were extracted for differential gene, developmental trajectories, GSVA and cell interaction analysis to observe the differences between regulatory T cells from normal tissues and cancer tissues. Results In terms of cell composition, there was great heterogeneity in different patients and different sample types. Compared with normal tissues, cancer tissues had higher proportion of regulatory T cells which were related to cell death, cell cycle and other pathways. Cell interaction analysis showed that regulatory T cells in cancer tissues interacted with other cells with lower frequency and greater intensity, and the interaction patterns changed significantly. Conclusion Regulatory T cells mainly exist in the microenvironment of gastric cancer. There are significant differences in the number and function of regulatory T cells between gastric cancer and normal tissues.

Key words: Gastric cancer, Single cell RNA sequencing, Cell composition, Regulatory T cells, Intercellular interaction

CLC Number: 

  • R735.2
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