Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (5): 60-67.doi: 10.6040/j.issn.1671-7554.0.2021.0278

• Current Advance of Basic and Clinical Medical Virology • Previous Articles     Next Articles

Positive distribution of four dominant enteroviruses associated with hand, foot and mouth disease and VP1 genotypes in Jinan, China, 2012-2017

Hengyun GUAN,Chunrong WANG,Lanzheng LIU*(),Huailong ZHAO,Aiying BAI,Huiyu JIANG,Hua DONG,Na LI,Xiaoying SHAN,Hongqi CHENG   

  1. Bacterial Disease Inspection Laboratory, Jinan Center for Disease Control and Prevention, Jinan 250021, Shandong, China
  • Received:2021-03-12 Online:2021-05-10 Published:2021-06-01
  • Contact: Lanzheng LIU E-mail:jncdcllz@jn.shangdong.cn

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Abstract:

Objective: To elucidate the dominant enteroviruses associated with hand, foot and mouth disease (HFMD) and the distributions of VP1 genotypes in Jinan City. Methods: Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect enterovirus A71 (EV-A71), coxsackievirus A16 (CV-A16), coxsackievirus A6 (CV-A6) and coxsackievirus A10 (CV-A10) in 4929 HFMD specimens from 2012 to 2017 in Jinan. According to different years, 65 virus isolates of HFMD were retrospectively collected, including 17 strains of EV-A71, 16 of CV-A16, 20 of CV-A6 and 12 of CV-A10. The VP1 gene of enteroviruses was amplified by RT-PCR and the nucleotide sequencing was performed. Nucleotide homology analyses were performed and phylogenetic trees were constructed on the VP1 gene. Results: The dominant enteroviruses of HFMD were CV-A16 (28.3%), EV-A71(27.3%), CV-A6(17.0%) and CV-A10(3.0%) in Jinan. The lengths of VP1 gene on CV-A16, EV-A71, CV-A6 and CV-A10 amplified by RT-PCR were 891 bp, 1 015 bp, 1 110 bp and 993 bp, respectively. The nucleotide homology was 93.0%-98.1% for EV-A71 strains, 87.4%-100.0% for CV-A16 strains, 87.6%-99.7% for CV-A6 strains, and 95.8%-99.4% for CV-A10 strains. The phylogenetic trees showed that EV-A71 strains belonged to C4a genotype in Jinan, CV-A16 strains had two genetic subtypes (B1b and B1a); CV-A6 strains located in clusters C, E and F, and CV-A10 strains located in cluster E. Conclusion: The dominant enteroviruses of HFMD are CV-A16, EV-A71, CV-A6 and CV-A10 from 2012 to 2017 in Jinan. The long-term dynamic monitoring of the distribution of VP1 genotypes is of great public health significance for improving the early warning sensitivity for HFMD.

Key words: Hand, foot and mouth disease, Enterovirus, VP1 gene, Phylogenetic analysis

CLC Number: 

  • R115

Table 1

Distribution of positive detection rates of four dominant enteroviruses associated with hand, foot and mouth disease in Jinan, 2012-2017"

年份 病例数 性别 年龄(中位数)(岁) 阳性检出数[n(%)] χ2 P
男 女 EV-A71 CV-A16 CV-A6 CV-A10
2012 859 515 344 0.3~44(3.0) 203(23.6) 368(42.8) 15(1.7) 39(4.5) 631.493a < 0.001
2013 848 489 359 0.2~21(3.0) 289(34.1) 148(17.5) 164(19.3) 12(1.4) 306.820a < 0.001
2014 935 545 390 0.3~23(3.0) 223(23.9) 357(38.2) 125(13.4) 62(6.6) 325.238a < 0.001
2015 756 453 303 0.3~30(3.1) 219(29.0) 82(10.8) 281(37.2) 5(0.7) 401.888a < 0.001
2016 741 433 308 0.1~30(3.0) 88(11.9) 372(50.2) 66(8.9) 30(4.0) 656.183a < 0.001
2017 790 467 323 0.1~30(3.0) 326(41.3) 66(8.4) 188(23.8) 2(0.3) 516.410a < 0.001

Fig.1

Nucleotide homology analysis (A) and phylogenetic tree construction (B) on VP1 gene of EV-A71 in Jinan, China ▲ represented EV-A71 strains in Jinan, China."

Fig.2

Nucleotide homology analysis (A) and phylogenetic tree construction (B) on VP1 gene of CV-A16 in Jinan, China ● represented CV-A16 strains in Jinan, China."

Fig.3

Nucleotide homology analysis (A) and phylogenetic tree construction (B) on VP1 gene of CV-A6 in Jinan, China ● represented CV-A6 strains in Jinan, China."

Fig.4

Nucleotide homology analysis (A) and phylogenetic tree construction (B) on VP1 gene of CV-A10 in Jinan, China ● represented CV-A10 strains in Jinan, China."

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