Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (5): 52-59.doi: 10.6040/j.issn.1671-7554.0.2021.0220

• Current Advance of Basic and Clinical Medical Virology • Previous Articles     Next Articles

Genetic characteristics of H10 subtype avian influenza virus in three southern provinces of China

Xiaoxu ZENG,Heng ZHANG,Ming JIAO,Tao CHEN,Xiyan LI,Weijuan HUANG,Jing YANG,Dayan WANG*()   

  1. Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
  • Received:2020-03-01 Online:2021-05-10 Published:2021-06-01
  • Contact: Dayan WANG E-mail:dayanwang@cnic.org.cn

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Abstract:

Objective: To explore the genetic characteristics of avian influenza virus (AIVs) between 2014 and 2019 in three southern provinces of China. Methods: A total of 30 231 environment samples isolated from Jiangxi, Hunan and Guangxi provinces from 2014 to 2019 were tested for flu A with real-time RT-PCR. If the testing results were positive, the samples were propagated in SPF chicken embryonated eggs. All positive AIVs isolations were sequenced. CLC, CD-HIT, MAFFT v7.037 and MEGA v6.06 software were used to analyze the influenza sequences. Results: Altogether 11 H10 subtype AIVs were isolated. Whole genome sequencing and phylogenetic analysis revealed that all genes were classified into Eurasian lineage, except 3 N8 genes in the North American lineage. JX24472 had close relationship with human infection H10N8 AIVs in its HA segment. The internal genome of 5 H10N3 subtype AIVs isolated from Jiangxi Province suggested their genome were diverse and complex. Molecular characteristic analysis of H10 subtype AIVs revealed that several mutations in key amino acid sites were associated with receptor binding sites, pathogenicity in mice and transmissibility in mammals. Conclusion: Phylogenetic analyses of the 11 H10 subtype AIVs isolated in this research suggested that they were in different clade with human infected H10N8 AIVs, except JX24472. Whereas the amino acid characterization analysis suggested that these H10 subtype viruses had the potential to infect human or poultry to make them sick. Therefore, it is necessary to strengthen the continuous surveillance of H10 subtype AIVs and prepare for the next potential pandemic.

Key words: Avian influenza virus, H10N3, H10N8, Phylogenetic analysis, Live poultry market, Surveillance

CLC Number: 

  • R373.1

Table 1

The sampling information of H10 subtype AIVs"

毒株名称 缩写 亚型 采样日期 采样场所 样本种类
A/chicken/Jiangxi/24472/2014 JX24472 H7/H10/N9 2014-04-29 活禽零售市场 鸡泄殖腔
A/environment/Jiangxi/07531/2015 JX07531 H10N3 2015-11-19 鸭养殖场 鸭粪便
A/environment/Jiangxi/07505/2015 JX07505 H10N3 2015-11-19 鸭养殖场 鸭笼具
A/environment /Jiangxi/07518/2015 JX07518 H10N3 2015-11-19 鸭养殖场 鸭粪便
A/environment /Jiangxi/07527/2015 JX07527 H10N3 2015-11-19 鸭养殖场 鸭粪便
A/environment/ Jiangxi/12590/2019 JX12590 H10N3 2019-06-18 批发市场 鸭粪便
A/environment/Guangxi/13425/2018 GX13425 H10N3 2018-12-24 活禽零售市场 脱毛机
A/environment/Guangxi/25019/2019 GX25019 H10N3 2019-01-02 活禽零售市场 鸭粪便
A/environment/Hunan/12257/2019 HN12257 H10N8 2019-08-19 活禽零售市场 禽咽拭子
A/environment/Hunan/12259/2019 HN12259 H10N8 2019-08-19 活禽零售市场 禽咽拭子
A/environment/Hunan/12263/2019 HN12263 H10N8 2019-08-19 活禽零售市场 鸭笼具

Fig.1

Phylogenetic tree for the HA"

Fig.2

Phylogenetic tree for the NA A: Phylogenetic tree for the N3; B: Phylogenetic tree for the N8."

Fig.3

Phylogenetic tree for the 6 internal genomes A: Phylogenetic tree for the PB2; B: Phylogenetic tree for the PB1; C: Phylogenetic tree for the PA; D: Phylogenetic tree for the NP; E: Phylogenetic tree for the M; F: Phylogenetic tree for the NS."

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