Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (10): 32-40.doi: 10.6040/j.issn.1671-7554.0.2021.0725

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Characteristics of bone marrow immune microenvironment in patients with acute myeloid leukemia based on single-cell RNA sequencing

WANG Yan1, ZHANG Yuhui1, HU Naibo1, TENG Guangshuai1, ZHOU Yuan2, BAI Jie1   

  1. 1. Department of Hematology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China;
    2. State Key Laboratory of Experimental Hematology, Institute of Hematology &
    Blood Diseases Hospital, National Clinical Research Center for Blood Diseases, Chinese Academy of Medical Sciences &
    Peking Union Medical College, Tianjin 300020, China
  • Published:2021-10-15

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Abstract: Objective To analyze the composition of bone marrow immune microenvironment in patients with acute myeloid leukemia(AML)by single cell sequencing technology, and to explore the role of different subsets of different types of immune cells in the occurrence and development of AML. Methods The scRNA-seq data(GSE116256)which met the requirements of this study were downloaded from GEO database and processed with the Seurat package of R language. The cell subsets were annotated by single R package and reference to previous studies, the proportion of different types of immune cells was calculated, and the differentially expressed genes(DEGs)of cell subsets between AML patients and normal controls were calculated. Gene ontology function annotation(GO)and Kyoto Encyclopedia of genes and genomes(KEGG)enrichment analysis on DEGs were performed to clarify the role of cell subsets in the progression of AML. Results Compared with the normal control group, AML patients had changed proportion of progenitor cells, monocytes, NK/T cells, dendritic cells and B cells. GO analysis indicated that the proliferation, differentiation, immune regulation and killing function of monocytes, dendritic cells, B cells and T cells were damaged. Conclusion There are big differences in the immune microenvironment between AML patients and healthy people, and these differences may be closely related to the occurrence and development of AML.

Key words: Acute myeloid leukemia, Single cell sequencing, Bone marrow microenvironment, Immune cells, Bioinformatics

CLC Number: 

  • R551.3
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