Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (2): 26-33.doi: 10.6040/j.issn.1671-7554.0.2020.0636

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Expression and activity mechanism of acetyl-CoA acyltransferase 1 in clear cell renal cell carcinoma based on bioinformatics

LI Canxuan, CHEN Jie   

  1. Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong, China
  • Published:2021-03-05

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Abstract: Objective To explore the expression of acetyl-CoA acyltransferase 1(ACAA1)in clear cell renal cell carcinoma(ccRCC)and investigate the correlative mechanism. Methods The GEO datasets(GSE36895, GSE66272, GSE53737), TCGA-KIRC dataset and Human Protein Atlas database were used to analyze the expression of ACAA1 in normal renal and ccRCC samples. Ualcan database was applied to analyze the correlation between ACAA1 mRNA and clinicopathological characteristics of ccRCC patients. The relationship between the ACAA1 mRNA levels and prognosis of ccRCC patients was determined using GEPIA database. Additionally, STRING and Metascape databases were used to construct a functional protein interaction network of ACAA1 and investigate its functional mechanism. Finally, the potential miRNAs that regulated ACAA1 were predicted by mirDIP and DIANA-TarBase v8 databases. Results ACAA1 expression level in ccRCC tissues was significantly lower than that in normal renal tissues, and ACAA1 mRNA expression was negatively correlated with the clinical stage and histological grade of ccRCC. Survival analysis showed that patients with low ACAA1 expression levels had significantly lower overall survival rate and disease-free survival rate than those with high ACAA1 expression levels. In addition, the ACAA1 protein interaction network was constructed through the STRING database and functional analysis showed that ACAA1 and its related genes were mainly involved in fatty acid β-oxidation, fatty acid metabolism, mitochondrial β-saturated fatty acid oxidation, peroxisomal protein transport, lipid regulation of metabolic processes and steroid metabolic processes. Finally, hsa-miR-25-3p and hsa-miR-363-3p might affect the occurrence and development of ccRCC by targeting ACAA1. Conclusion ACAA1 is down-expressed in ccRCC and related to its occurrence and development, which may provide theoretical assistance for in-depth study of the mechanism of ACAA1 in ccRCC.

Key words: Clear cell renal cell carcinoma, Acetyl-CoA acyltransferase 1, Bioinformatics

CLC Number: 

  • R737.11
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