JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (1): 7-10.doi: 10.6040/j.issn.1671-7554.0.2015.211

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Neuroprotective effect of flavanoids extracted from onion on retinal ganglion cells with diabetes mellitus

HAO Fengqin, LI Na   

  1. Eye Center, Clinical Institute of Weifang Medical University, Weifang 261031, Shandong, China
  • Received:2015-02-26 Online:2016-01-11 Published:2016-01-11

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Abstract: Objective To detect the apoptosis of the retinal ganglion cells(RGCs)in rats with diabetes mellitus and expression of the apoptosis-related genes, then to explore the neuroprotective effect of flavanoids extracted from onion on RGCs. Methods Diabetic rats were induced by streptozotocin. Fifty rats were randomly divided into five groups: normal control group, diabetic model group(DM group), low-dose flavanoids extracted from onion group(I group), medium-dose flavanoids extracted from onion group(II group), and high-dose flavanoids extracted from onion group(III group), ten in each group. Rats in I, II and III groups were treated with flavanoids extracted from onion once each day. The apoptosis of RGCs were measured by TUNEL assay. The expression levels of Bcl-2 and Bax were measured by LSAB. Results Compared with normal control group, the blood sugar of rats in DM group were significantly increased, the body weight were significantly reduced(P<0.01), expression levels of Bcl-2 and Bax were enhanced, and the numbers of apoptosis RGCs were increased significantly(P<0.01). Compared with DM group, the blood sugar of rats in II and III groups were significantly decreased(P<0.01), expression level of Bcl-2 was enhanced but that of Bax was decreased(P<0.05, P<0.01), the numbers of apoptosis RGCs were decreased significantly(P<0.01), and the body weight of rats in III group were significantly increased(P<0.05). Conclusion Flavanoids extracted from onion can induce the expression of Bcl-2 while inhibit that of Bax, reduce the apoptosis of RGCs, and protect RGCs neurologically.

Key words: Retinal ganglion cells, Onion, Flavanoids, Apoptosis, Diabetic retinopathy

CLC Number: 

  • R774.1
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