JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (1): 1-6.doi: 10.6040/j.issn.1671-7554.0.2015.291

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Gene microarray analysis of mature dendritic cells cultured with hepatocellular carcinoma cells

YANG Hui, DONG Rong, XUE Hui, HU Zuquan, ZENG Zhu   

  1. College of Biology and Engineering, Guizhou Medical University, Guiyang 550004, Guizhou, China
  • Received:2015-03-19 Online:2016-01-11 Published:2016-01-11

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Abstract: Objective To detect the effects of hepatocellular carcinoma cells on the transcription of functional genes in maturate dendritic cells(mDCs). Methods Peripheral blood mononuclear cells(PBMCs)were enriched from freshly harvested whole blood by immunomagnetic beads. MDCs were induced by GM-CSF, IL-4 and TNF-α in vitro. Then, mDCs were cultured with(co-cultured group)or without(control group)hepatoma cells. Forty-eight hours later, total RNA of mDCs was collected and the expressions of functional genes were analyzed by using human dendritic cells gene array. Results There were 663 genes significantly regulated(compared with control group, fold changes≥ 2)in co-cultured group, of which 290 were up-regulated and 373 were down-regulated. These genes were associated with the functions of signal transduction, cell metabolism, cell cycle, and so on. Conclusion Hepatocellular carcinoma cells affect the transcription of some functional genes in mDCs, which thus affects its functions, proliferation, differentiation and apoptosis.

Key words: Tumor microenvironment, Microarrary, Liver neoplasms, Dendritic cells

CLC Number: 

  • R318.04
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