JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 23-27.doi: 10.6040/j.issn.1671-7554.0.2015.083

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The role of miR-485-5p in human colorectal cancer HCT116 cell invasion by regulating survivin expression

ZENG Renren, ZHANG Junhua, ZHANG Yinxu   

  1. Department of General Surgery, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning, China
  • Received:2015-01-23 Revised:2015-04-23 Online:2015-06-10 Published:2015-06-10

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Abstract: Objective To identify the role of miR-485-5p in human colorectal cancer HCT116 cell invasion. Methods HCT116 cells were cultured in vitro, and cellular morphology was observed with phase contrast microscope. The miR-485-5p was predicted with bioinformatics and identified with dual luciferase report system. Expressions of miR-485-5p and survivin were determined with Real-time PCR and Western blotting after transfection of miR-485-5p mimics. The invasion of HCT116 cells was detected in vitro with transwell chamber. Results Survivin expression was positive as detected by immunofluorescence cytochemistry. Dual luciferase reporter system indicated that miR-485-5p inhibited survivin expression by binding to 3'UTR of survivin mRNA. Real-time PCR and Western blotting showed that over-expression of miR-485-5p down-regulated survivin expression. The invasion of HCT116 cells was suppressed by over-expression of miR-485-5p. Conclusion The miR-485-5p may negatively regulate survivin expression by binding to its 3'UTR in human colorectal cancer HCT116 cells and inhibit cell invasion.

Key words: Colorectal neoplasm, MiRNA, Survivin, HCT116 cells

CLC Number: 

  • R735.3
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