JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 75-79.doi: 10.6040/j.issn.1671-7554.0.2015.1131

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Correlation between TLR4, MyD88 and ERK1/2 expressions and EBV infected colorectal cancer

CAO Kai, WANG Chaoqun, ZHENG Maojin, WU Yongping   

  1. Department of Pathology, Xuzhou Medical College, Xuzhou 221004, Jiangsu, China
  • Received:2015-11-19 Online:2016-07-10 Published:2016-07-10

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Abstract: Objective To explore the correlation between toll-like receptor 4(TLR4), myeloid differentiation primary response gene 88(MyD88)and extracellular signal-regulated protein kinases 1 and 2(ERK1/2)expressions and Epstein-Barr virus(EBV)infection in colorectal cancer. Methods Epstein-Barr virus-encoded small RNA(EBER)expression was detected by in situ hybridization using microarray chips containing tissues from 336 cases of colorectal cancer and 103 cases of normal mucosa. The correlation between EBER expression and clinicopathological parameters and prognosis of colorectal cancer was analyzed. Additionally, TLR4, MyD88 and ERK1/2 expressions were detected using SP immunohistochemistry. The correlation between these three factors and EBV infection in colorectal cancer was analyzed. Results TLR4, MyD88, ERK1/2 and EBER expressions were increased in the 336 cases of colorectal cancer compared to the controls(P<0.05). EBER expression was positively correlated with the female gender, serosal infiltration, lymph node metastasis and clinical TNM stages Ⅲ and Ⅳ(P<0.05). Prognostic analysis of the 336 cases of colorectal cancer demonstrated that EBER expression was closely associated with low disease-free survival and overall survival rates(P<0.05). In addition, TLR4, MyD88 and ERK1/2 were expressed in 22(P=0.025), 25(P=0.028)and 19(P=0.092)of the 25 EBER-positive colorectal cancer tissues, respectively. Conclusion EBV infection is closely associated with the progress of colorectal cancer, particularly in female patients, and represents an independent risk factor for the prognosis. Upregulation of TLR4 and MyD88 expressions may play an important role in EBV infected colorectal cancer.

Key words: Colorectal neoplasm, Epstein-barr virus, Myeloid differentiation factor 88, Toll-like receptor 4, Extracellular regulated kinase

CLC Number: 

  • R735.3
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