JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 18-22.doi: 10.6040/j.issn.1671-7554.0.2015.245

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Transportation and absorption of Alternaria mycotoxins in Caco-2 cells

ZHAO Kai1,2, HAN Chunhui2, LIN Xiaohui2, JIANG Tao2, YANG Dajin2, SONG Jingdong3, LI Fengqin2   

  1. 1. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China;
    2. Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China;
    3. Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
  • Received:2015-03-07 Revised:2015-04-16 Online:2015-06-10 Published:2015-06-10

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Abstract: Objective To develop and evaluate the Caco-2 cell model and to explore the transportation and absorption of 3 Alternaria mycotoxins. Methods Caco-2 cell model was established by inoculating Caco-2 cell on the polycarbonate membrane attached to the transwell plates and evaluated on the basis of transepithelium electrical resistance (TEER), activity of alkaline phosphatase (ALP), apparent permeability coefficient (Papp) of fluorescein and cytomorphology. Transportation and absorption of 3 Alternaria toxins including alternariol (AOH), alternariol methyl ether (AME) and tenuazonic acid (TeA) in different concentrations and at different time points were studied by determining the difference in toxin concentrations between apical side and basolateral side of the cell line. Results The TEER exceeded 300±15 Ω·cm2 and ALP activity was statistically different (P<0.01) between apical side and basolateral side after 21 days' incubation. Clear microvilli and tight intercellular junctions were observed with transmission electron microscope. Papps of AOH, AME and TeA varied between 1×10-5cm/s and 10×10-5cm/s. Transportation and absorptionof 3 Alternaria toxins at different concentrations ranged from 1.5% to 63.0%. Conclusion Intact and tight cell model was developed for Alternaria toxins transportation and absorption. AOH, AME and TeA were toxins easily or moderately absorbed by human beings.

Key words: Alternaria mycotoxins, Absorption, Caco-2 cell, Transportation, Model

CLC Number: 

  • R155.5
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