JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 18-22.doi: 10.6040/j.issn.1671-7554.0.2015.245

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Transportation and absorption of Alternaria mycotoxins in Caco-2 cells

ZHAO Kai1,2, HAN Chunhui2, LIN Xiaohui2, JIANG Tao2, YANG Dajin2, SONG Jingdong3, LI Fengqin2   

  1. 1. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China;
    2. Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China;
    3. Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
  • Received:2015-03-07 Revised:2015-04-16 Online:2015-06-10 Published:2015-06-10

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Abstract: Objective To develop and evaluate the Caco-2 cell model and to explore the transportation and absorption of 3 Alternaria mycotoxins. Methods Caco-2 cell model was established by inoculating Caco-2 cell on the polycarbonate membrane attached to the transwell plates and evaluated on the basis of transepithelium electrical resistance (TEER), activity of alkaline phosphatase (ALP), apparent permeability coefficient (Papp) of fluorescein and cytomorphology. Transportation and absorption of 3 Alternaria toxins including alternariol (AOH), alternariol methyl ether (AME) and tenuazonic acid (TeA) in different concentrations and at different time points were studied by determining the difference in toxin concentrations between apical side and basolateral side of the cell line. Results The TEER exceeded 300±15 Ω·cm2 and ALP activity was statistically different (P<0.01) between apical side and basolateral side after 21 days' incubation. Clear microvilli and tight intercellular junctions were observed with transmission electron microscope. Papps of AOH, AME and TeA varied between 1×10-5cm/s and 10×10-5cm/s. Transportation and absorptionof 3 Alternaria toxins at different concentrations ranged from 1.5% to 63.0%. Conclusion Intact and tight cell model was developed for Alternaria toxins transportation and absorption. AOH, AME and TeA were toxins easily or moderately absorbed by human beings.

Key words: Alternaria mycotoxins, Absorption, Caco-2 cell, Transportation, Model

CLC Number: 

  • R155.5
[1] Li Fq, Yoshizawa T. Alternaria mycotoxins in weathered wheat from China[J]. J Agric Food Chem, 2000, 48(7): 2920-2924.
[2] Ostry V. Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs[J]. World Mycotoxin Journal, 2008, 1(2): 175-188.
[3] Zhao K, Shao B, Yang D, et al. Natural occurrence of four alternaria mycotoxins in tomato- and citrus-Based Foods in China[J]. J Agric Food Chem, 2015, 63: 343-348.
[4] 林肖惠, 胡骁, 李凤琴. Caco-2细胞模型的建立及其在真菌毒素吸收率研究中的应用[J]. 卫生研究, 2014, 43(6): 923-928. LIN Xiaohui, HU Xiao, LI Fengqin. Development and application of Caco-2 cell lines transfering model for assessing the bioavailability of mycotoxins[J]. Journal of Hygien Research, 2014, 43(6): 923-928.
[5] 雷激, 张明秋, 黄承钰, 等. 用体外消化/Caco-2细胞模型观察维生素C和柠檬酸对铁生物利用的影响[J]. 南方医科大学学报, 2008, 28(10): 1743-1747. LEI Ji, ZHANG Mingqiu, HUANG Chengyu, et al. Effects of ascorbic acid and citric acid on iron bioavailability in an in vitro digestion/Caco-2 cell culture model[J]. Journal of Southern Medical University, 2008, 28(10): 1743-1747.
[6] 蔡润兰, 王敏, 齐云, 等. Caco-2细胞模型验证指标的选择与评判[J].中国药学杂志, 2008, 43(24): 1871-1875. CAI Runlan, WANG Min, QI Yun, et al. SeIection and utilization on the evaluation criteria of Caco-2 cell model[J]. Chinese Pharmaceutical Journal, 2008, 43(24): 1871-1875.
[7] EFSA on Contaminants in the Food Chain (CONTAM). Scientific opinion on the risks for animal and public health related to the presence of Alternaria toxins in feed and food[J/OL]. EFSA Journal, 2011, 9(10): 2407. http: www.efsa.europa.eu/efsajournal. 2011-10-26.
[8] Artursson P, Palm K, Luthman K. Caco-2 monolayers in experimental and theoretical predictions of drug transport[J]. Adv Drug Deliv Rev, 2001, 46(1-3): 27-43.
[9] Konsoula R, Barle F A. Correlation of in vitro cytotoxicity with paracellular permeability in Caco-2 cell[J]. Toxicol In Vitro, 2005, 19(5): 675-684.
[10] Masungi C, Borremans C, Willems B, et al. Usefulness of a novel Caco-2 cell perfusion system. I. In vitro prediction of the absorption potential of passively diffused compounds[J]. J Pharm Sci, 2004, 93(10): 2507-2521.
[11] Masungi C, Mensch J, Willems B, et al. Usefulness of a novel Caco-2 cell perfusion system II. Characterization of monolayer properties and peptidase activity[J]. Pharmazie, 2009, 64(1): 36-42.
[12] Yamashita S, Konishi K, Yamazaki Y, et al. New and better protocols for a short-term Caco-2 cell culture system[J]. J Pharm Sci, 2002, 91(3):669-679.
[13] 杨秀伟, 杨晓达, 王莹, 等. 中药化学成分肠吸收研究中Caco-2细胞模型和标准操作规程的建立[J]. 中西医结合学报, 2007, 5(6): 634-641. YANG Xiuwei, YANG Xiaoda, WANG Ying, et al. Establishment of Caco-2 cell monolayer model and standard operation procedure for assessing intestinal absorption of chemical components of traditional Chinese medicine[J]. Journal of Chinese Integrative Medicine, 2007, 5(6): 634-641.
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