JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (6): 13-17.doi: 10.6040/j.issn.1671-7554.0.2014.515

Previous Articles     Next Articles

Assessment of abdominal lymph node metastasis of VX2 hepatic cancer rabbits with percutaneous transhepatic lymphosonography

LI Xuefeng1, WANG Shuanglong1, YU Mei2, CUI Hong3, SUN Yuanyuan4, DONG Lei4   

  1. 1. Department of Ultrasound, First People's Hospital of Jining City, Jining 272100, Shandong, China;
    2. Department of Ultrasound, Hospital for Infectious Diseases of Jining City, Jining 272031, Shandong, China;
    3. Special Clinical Laboratory, Central Hospital of Taian City, Taian 271000, Shandong, China;
    4. Department of Ultrasound, General Hospital of Jinan Military Command, Jinan 250031, Shandong, China
  • Received:2014-08-06 Revised:2015-03-19 Online:2015-06-10 Published:2015-06-10

PDF (PC)

129

Abstract: Objective To investigate the applications of percutaneous transhepatic lymphosonography in assessing abdominal lymph node metastasis of VX2 hepatic cancer rabbits. Methods A total of 30 rabbits with VX2 tumor were divided into sham group (group A, n=5) and hepatic cancer group (group B, n=25). SonoVue was injected into the liver parenchyma inside and around the VX2 cancer to observe the normal heptic tissues, cancerous tissues and pericancerous tissues dynamically. The changes were quantatively analyzed. Results Of the 25 rabbits in group B, 11 (group B2) had lymph node metastasis while 14 (group B1) had not. Percutaneous transhepatic lymphosonography showed the liver tissues could be divided into 4 phases, interstitial space, defusing, plateau and dissipating phase. SonoVue disappeared in cancerous tissues faster than in pericancerous tissues, in which SonoVue sustained for 20 minutes. There were significant differences among normal heptic tissues, cancerous tissues and pericancerous tissues in plateau intensity(PTI)(P<0.01), and PTI in cancerous tissues was the greatest, followed by pericancerous tissues and normal heptic tissues. There were significant differences in pericancerous tissues of group A, B1 and B2, and PTI in group B2 was the greatest, followed by group B1 and group A (P<0.05). Conclusion Percutaneous transhepatic lymphosonography can provide detailed information of abdominal lymph node metastasis of hepatic cancer, which is useful for the choice of treatment and prediction of therapeutic effects.

Key words: Percutaneous transhepatic lymphosonography, Lymph node metastasis, Hepatic cancer

CLC Number: 

  • R445.1
[1] Teramoto K, Kawamura T, Okamoto H, et al. Percutaneous transhepatic lymphography method to image and treat intra-abdominal lymph node metastasis in patients with unresectable hepatobiliary pancreatic cancer[J]. Surgery, 2002, 131(5): 529-533.
[2] Goldberg BB, Merton DA, Liu JB, et al. Sentinel lymph nodes in a swine model with melanoma: contrast-enhanced lymphatic US[J]. Radiology, 2004, 230(3): 727-734.
[3] 梅铭惠, 陈谦, 杨景红, 等. 肝癌淋巴结转移—外科治疗面临的挑战之一[J]. 中华普通外科学文献: 电子版, 2007, 1(2): 89-92. MEI Minghui, CHEN Qian, YANG Jinghong, et al. A challenge to surgical treatments of liver cancer[J]. Chinese Archives Of General Surgery: Electronic Version, 2007, 1(2): 89-92.
[4] 祁付珍, 张平, 顾殿华, 等. 肝门淋巴结清扫术在原发性肝癌治疗中的价值[J]. 实用癌症杂志, 2009, 24(3): 267-269. QI Fuzhen, ZHANG Ping, GU Dianhua, et al. The role of lymphadenectomy in the treatment of primary liver cancer[J]. The Practical Journal of Cancer, 2009, 24(3): 267-269.
[5] 王凯峰, 叶胜龙, 宋丽杰, 等. 不同转移潜能的小鼠肝癌淋巴道转移和淋巴管生成的研究[J]. 中华肝脏病杂志, 2006, 14(3): 187-191. WANG Kaifeng, YE Shenglong, SONG Lijie, et al. The relationship between lymphangiogenesis and lymphatic metastasis in murine hepatic carcinoma of high and low metastatic potentialities[J]. Chin J Hepatolm, 2006, 14(3): 187-191.
[6] 黄志强, 黄晓强. 肝胆胰区的淋巴外科[M]. 北京: 人民军医出版社, 2005: 515-521.
[7] 周宏志, 顾晓明, 胡敏, 等. 小鼠肝癌H22诱导淋巴管生成的体外研究[J]. 中华肿瘤杂志, 2003, 25(1): 39-42. ZHOU Hongzhi, GU Xiaoming, HU Min, et al. Induction of lymphangiogenesis by mouse ascites hepatoma cell H22 in vitro[J]. Clin J Oncol, 2003, 25(1): 39-42.
[8] 李晓冬, 赵玲辉, 张雅芳, 等. 小鼠肝癌细胞移植瘤内淋巴管的形态与VEGF-C、VEGFR-3的表达[J]. 哈尔滨医科大学学报, 2005, 39(2): 135-137. LI Xiaodong, ZHAO Linghui, ZHANG Yafang, et al. Morphological feature of lymphatics in transplanted hepalocarcinoma in mice and the expression of VEGF-C and VEGFR-3[J]. Journal of Harbin Medical University, 2005, 39(2): 135-137.
[9] 赵恩阳, 李玉兰, 杨慧科, 等. 小鼠肝癌移植瘤及其淋巴管的研究[J]. 黑龙江医学, 2007, 31(12): 911-913. ZHAO Enyang, LI Yulan, YANG Huike, et al. Study on the liver transplanted tumor of mouse and its lymphatic vessels[J]. Hei Long Jiang Medical Journal, 2007, 31(12): 911-913.
[10] 吉胜朴, 李强. 肝癌淋巴结转移机制研究进展[J]. 中国肿瘤临床, 2009, 36(18): 1076-1079. JI Shengpu, LI Qiang. Advances in research on the mechanism of lymph node metastasis of liver cancer[J]. Chinese Journal of Clinical Oncology, 2009, 36(18): 1076-1079.
[11] 孙平, 孙成, 李东起, 等. 肝脏器官内淋巴管的研究进展[J]. 牡丹江医学院学报, 2004, 25(2): 42-45. SUN Ping, SUN Cheng, LI Dongqi, et al. Advances in research on the lymphtic of liver[J]. Journal Of Mudanjiang Medical College, 2004, 25(2): 42-45.
[12] Yoshiko K, Kumiko A, Takashi N, et al. Real-time imaging of the lymphatic channels and sentinel lymph nodes of the stomach using contrast-enhanced ultrasonography with Sonazoid in a porcine model[J]. Japanese Cancer Association, 2011, 102(11): 1340-1349.
[13] Barry BG, Daniel AM, Ji-Bin Liu, et al. Contrast-enhanced ultrasound imaging of sentinel lymph nodes after peritumoral administration of Sonazoid in a melanoma tumor animal model[J]. J Ultrasound Med, 2011, 30: 441-453.
[14] 董磊, 王双龙, 张彦, 等. 经皮经肝淋巴超声造影检测兔VX2肝癌前哨淋巴结的实验研究[J]. 中华超声影像学杂志, 2013, 22(2): 71-74. DONG Lei, WANG Shuanglong, ZHANG Yan, et al. Experimental study in detecting sentinel lymph nodes by percutaneous transhepatic lymphosonography in VX2 hepatic cancer rabbit[J]. Chinese Journal of Ultrasonography, 2013, 22(2): 71-74.
[15] 李天满, 陈谦, 廖维甲, 等. 肝细胞癌中podoplanin的表达及其与淋巴道转移关系的研究[J]. 中国现代医学杂志, 2010, 20(7): 984-987. LI Tianman, CHEN Qian, LIAO Weijia, et al. Investigation of relation between expression of podoplanin and lymphatic metastasis in human hepatocellular carcinoma[J]. China Journal of Modern Medicine, 2010, 20(7): 984-987.
[1] LI Xingkai, LIU Zhanye, JIANG Yunfeng, LI Jun. Clinicopathological factors and prognosis between primary central and peripheral lung squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 73-78.
[2] YUAN Zhiping, MOU Huaping, CHEN Jialian, DENG Li. Expressions and significances of serum hepatic cancer-derived growth factor and vascular endothelial growth factor in patients with non-small cell lung cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 63-66.
[3] XIAO Jin-hua1, WANG Ya-ping1, ZHU Hua-yan1, PAN Yu-hong1, XU Qiu-lan2. Expression of serum HMGB1 and MMP-9 in early gastric cancer patients and its clinical significance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 70-73.
[4] MA Yu-hua1,2, ZHENG Yan3, JIA Yan-fei3, WANG Yun-shan3. GFR and DEC1 protein co-expression promotes lymph node
metastasis in lung adenocarcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(3): 24-28.
[5] . Metastases associated with proteins in breast cancer by proteomics [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(7): 61-64.
[6] WANG Jing-yuan,FU Qin-ye,DING Yin-lu,LIANG Si-feng,ZHANG Jian-liang. COX-2 expression in rectal adenocarcinoma and its relationgship with lymphangiogenesis and lymph node metastasis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(7): 711-713.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd