JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 1-8,28.doi: 10.6040/j.issn.1671-7554.0.2014.273

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Pro-atherosclerotic effects of streptozotocin-induced diabetes combined with chow diet on apoE-/-mice

SUN Hui, ZHAO Lei, ZHEN Xi, WANG Shasha, HUANG Shanying, HE Hong, HU Qin   

  1. Department of Cardiology, Qilu Hospital of Shandong University;
    Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Jinan 250012, Shandong, China
  • Received:2014-04-29 Revised:2014-09-14 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To explore the effects of streptozotocin(STZ)-induced diabetes combined with chow diet on atherosclerotic lesions of apoE-/-mice. Methods After 2 weeks of chow diet, 60 male apoE-/-mice (10 weeks old) were randomly divided into two groups. Diabetic apoE-/-mice (n=30) were constructed by intraperitoneal injection of low-dose STZ for 5 consecutive days. ApoE-/-mice (n=30) injected with citrate buffer liquid served as non-diabetic controls. All mice were fed with chow diet until sacrifice (23 weeks old). Serum lipid, inflammation cytokines and blood glucose were measured. Atherosclerotic plaques in the aorta and aortic root were examined with HE staining and Oil-Red-O staining. The mRNA and protein levels of vascular smooth muscle cells (SMCs), macrophages, MMP-9/TIMP-1, TNF-α and MCP-1 in the plaques were detected with immunohistochemical staining, RT-PCR and Western blotting, respectively. Results The non-diabetic apoE-/-mice showed slight atherosclerotic lesions, while diabetic apoE-/-mice had significantly aggravated atherosclerotic lesions. Compared with non-diabetic mice, diabetic apoE-/-mice had markedly larger size of plaques, in which lipid, macrophages, type Ⅰ and Ⅲ collagens and SMCs increased,while SMCs in fiber cap decreased, and atherosclerotic plaque instability index rose. Moreover, serum IL-6 and MIF levels, TNF-α, MCP-1, MMP-2 and MMP-9 mRNA all increased while TIMP-1 decreased. Conclusion STZ-induced diabetic apoE-/-mice fed with chow diet show deteriorated atherosclerotic lesions and relatively unstable plaques.

Key words: Diabetes mellitus, Atherosclerosis, ApoE-/-mice, Streptozotocin

CLC Number: 

  • R541.4
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