Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (7): 57-64.doi: 10.6040/j.issn.1671-7554.0.2017.1216

Previous Articles    

Correlation of Yes-associated protein and the pathological factors of colon cancer

GAO Zhenwen, YANG Shuping, XIE Xuexue, SONG Wei   

  1. Department of Minimally Invasive Tumor Therapies, Shandong Provincial Hospital Affiliated toShandong University, Jinan 250021, Shandong, China
  • Published:2022-09-27

PDF (PC)

50

Abstract: Objective To investigate the expression of Yes-associated protein(YAP), a new transcriptional coactivator, and its correlation with the clincopathological factors and metastases of colon cancer. Methods A total of 104 paraffin embedded tissues were selected, including 80 colon cancer tissues and 24 metastatic tissues. The protein expressions of YAP and N-cadherin in the tissues were detected with immunohistochemical method. The expressions of YAP, E-cadherin and N-cadherin in six colon cancer cell lines were determined with qRT-PCR and Western blotting. Results There was no significant difference of YAP positive expression between the primary and metastatic colon cancer(P>0.05), while the positive expression of N-cadherin was significantly higher in primary colon cancer than in the metastatic cancer(P<0.05). There was positive correlation between YAP and N-cadherin in primary colon cancer(r=0.5003, P<0.001), but not in the metastatic cancer(r=0.0325, P>0.05). The positive expressions of YAP and N-cadherin were correlated with serosal invasion and distant metastases(P<0.05), not with anatomical and histological type, differentiation, tumor size, lymph node metastases, microvascular invasion, left-sided and right-sided colon cancer (P>0.05). In the six colorectal cancer cell lines(SW480, SW620, SW1116, DLD-1, HT29 and HCT116), there were no differences in the mRNA and protein expressions of YAP(P>0.05). E-cadherin was highly expressed in HT29 and 山 东 大 学 学 报 (医 学 版)56卷7期 -高振文,等.Yes相关蛋白与结肠癌病理因素的相关性 \=-SW1116, and N-cadherin was highly expressed in HCT116 and SW620. Conclusion YAP can promote the development and metastasis of colon cancer by regulating the epithelial-mesenchymal transition.

Key words: Yes-associated protein, Colon cancer, Epithelial-mesenchymal transition, N-cadherin, Primary tumor, Metastasis

CLC Number: 

  • R735.3
[1] Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2): 115-132.
[2] Siegel R, Desantis C, Jemal A, et al. Colorectal cancer statistics, 2014[J]. CA Cancer J Clin, 2014, 64(2): 104-117.
[3] Pan D. Hippo signaling in organ size control[J]. Genes Dev, 2007, 21(8): 886-897.
[4] Pan D. The hippo signaling pathway in development and cancer[J]. Dev Cell, 2010, 19(4): 491-505.
[5] Maugeri-Saccà M, Barba M, Pizzuti L, et al. The Hippo transducers TAZ and YAP in breast cancer: oncogenic activities and clinical implications[J]. Expert Rev Mol Med, 2015, 17: e14. doi: 10.1017/erm.2015.12.
[6] Wang S, Li H, Wang G, et al. Yes-associated protein(YAP)expression is involved in epithelial-mesenchymal transition in hepatocellular carcinoma[J]. Clin Transl Oncol, 2016, 18(2): 172-177.
[7] Zhou Z, Zhu JS, Gao CP, et al. siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice[J]. Oncol Lett, 2016, 11(4): 2806-2814.
[8] Chaib I, Karachaliou N, Pilotto S, et al. Co-activation of STAT3 and YES-Associated Protein 1(YAP1)Pathway in EGFR-Mutant NSCLC[J]. J Natl Cancer Inst, 2017, 109(9). doi: 10.1093/jnci/djx014.
[9] Cho SY, Kim K, Park MS, et al. Expression of Yes-associated protein 1 and its clinical significance in ovarian serous cystadenocarcinoma[J]. Oncol Rep, 2017, 37(5): 2620-2632.
[10] Lamouille S, Xu J, Derynck R, et al. Molecular mechanisms of epithelial-mesenchymal transition[J]. Nat Rev Mol Cell Biol, 2014, 15(3): 178-196.
[11] Harvey KF, Zhang X, Thomas DM, et al. The Hippo pathway and human cancer[J]. Nat Rev Cancer, 2013, 13(4): 246-257.
[12] Zanconato F, Cordenonsi M, Piccolo S, et al. YAP/TAZ at the roots of cancer[J]. Cancer Cell, 2016, 29(6): 783-803.
[13] Zhang H, Liu CY, Zha ZY, et al. TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition[J]. J Biol Chem, 2009, 284(20): 13355-13362.
[14] Pefani DE, Pankova D, Abraham AG, et al. TGF-β targets the hippo pathway scaffold RASSF1A to facilitate YAP/SMAD2 nuclear translocation [J]. Mol Cell, 2016, 63(1): 156-166.
[15] Perumal N, Perumal M, Kannan A, et al. Morin impedes Yap nuclear translocation and fosters apoptosis through suppression of Wnt/β-catenin and NF-κB signaling in Mst1 overexpressed HepG2 cells [J]. Exp Cell Res, 2017, 355(2): 124-141.
[16] Zhang Y, Yuan J, Zhang X, et al. Angiomotin promotes the malignant potential of colon cancer cells by activating the YAP-ERK/PI3K-AKT signaling pathway [J]. Oncol Rep, 2016, 36(6): 3619-3626.
[17] Lee GH, Malietzis G, Askari A, et al. Is right-sided colon cancer different to left-sided colorectal cancer?-a systematic review[J]. Eur J Surg Oncol, 2015, 41(3): 300-308.
[18] Zeisberg M, Neilson EG. Biomarkers for epithelial-mesenchymal transitions [J]. J Clin Invest, 2009, 119(6): 1429-1437.
[19] Iwatsuki M, Mimori K, Yokobori T, et al. Epithelial-mesenchymal transition in cancer development and its clinical significance[J]. Cancer Sci, 2010, 101(2): 293-299.
[20] Wang L, Shi S, Guo Z, et al. Overexpression of YAP and TAZ is an independent predictor of prognosis in colorectal cancer and related to the proliferation and metastasis of colon cancer cells[J]. PLoS One, 2013, 8(6): e65539. doi: 10.1371/journal.pone.0065539.
[21] Vassilev A, Kaneko KJ, Shu H, et al. TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm[J]. Genes Dev, 2001, 15(10): 1229-1241.
[22] Scoazec JY. Epithelial-mesenchymal transition and colon cancer[J]. Ann Pathol, 2009, 29 Spec No 1:S63-64. doi: 10.1016/j.annpat.2009.07.037.
[23] Nawa T, Kato J, Kawamoto H, et al. Differences between right- and left-sided colon cancer in patient characteristics, cancer morphology and histology[J]. J Gastroenterol Hepatol, 2008, 23(3): 418-423.
[24] 刘传玲, 张晓东. 左右半结肠癌的差异及临床治疗理念[J]. 中国肿瘤临床, 2016, 43(18): 787-791. LIU Chuanling, ZHANG Xiaodong. Comparison and treatment of left- and right-side colon cancer [J]. Chinese Journal of Clinical Oncology, 2016, 43(18): 787-791.
[25] Shao DD, Xue W, Krall EB, et al. KRAS and YAP1 converge to regulate EMT and tumor survival [J]. Cell, 2014, 158(1): 171-184.
[1] SHEN Xiaochang, SUN Yiqing, YAN Lei, ZHAO Xingbo. Expression of aryl hydrocarbon receptor nuclear translocator-like 2 in endometrial cancer [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 74-80.
[2] CHEN Zhaobo, FANG Min, XUE Haoran, LIU Chunyan. Deubiquitinase USP35 promotes the cells migration and invasion of non-small cell lung cancer [J]. Journal of Shandong University (Health Sciences), 2022, 60(4): 30-37.
[3] ZHONG Lili, SHENG Ying, GUO Jianghong, YANG Shuangjian, HE Yijing. LncRNA-UCA1 effects invasion and metastasis of trophoblast cells by targeting miR-182-5p [J]. Journal of Shandong University (Health Sciences), 2022, 60(3): 76-82.
[4] ZHANG Qian, QIN Mingming, HE Xuejia, CAI Qiujing, ZHANG Yamin, LI Qingsu, ZHU Weiwei. Effects of calcitriol on EMT induced by TGF-β1 in asthma [J]. Journal of Shandong University (Health Sciences), 2021, 59(7): 10-18.
[5] GAO Jinmei, ZHANG Xianglian, LIU Tieju. Correlation between plasma D-dimer and metastasis in 109 cases of bladder cancer [J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 98-102.
[6] ZHEN Qiulai, LYU Xinran, YE Hui, DING Xuchao, CHAI Xiaoxue, HU Xin, ZHOU Ming, CAO Lili. Predicting colon cancer prognosis genes and clinical application value based on TCGA database [J]. Journal of Shandong University (Health Sciences), 2021, 59(1): 64-71.
[7] WANG Baojin, ZHAO Xinxin, LI Xia, MA Qian, WANG Xinyue, SUN Yang, SHI Zhongna. miR-203 targeting Survivin to inhibit the proliferation, migration and invasion of ovarian cancer cells [J]. Journal of Shandong University (Health Sciences), 2020, 58(12): 23-28.
[8] LIU Yaxiao, SHI Benkang. Effects of capsaicin on the invasion and metastasis of bladder cancer [J]. Journal of Shandong University (Health Sciences), 2018, 56(3): 34-40.
[9] LI Xingkai, LIU Zhanye, JIANG Yunfeng, LI Jun. Clinicopathological factors and prognosis between primary central and peripheral lung squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 73-78.
[10] ZENG Haiyan, LI Rui, SUN Xindong, XIE Peng, MENG Xue, FAN Bingjie, LI Wanlong, YUAN Shuanghu. Association analysis of brain metastases after prophylactic cranial irradiation in local disease small cell lung cancer: a bicenter study [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 61-66.
[11] LI Yang, HE Chuang, CHEN Yuxiao, YANG Li, LI Liangshan, LI Tingyuan, HUANG Xuequan. CT-guided interstitia 125I seed implantation in the treatment of long bone metastasis: a primary clinic study [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(2): 50-54.
[12] LIU Huiling, WANG Xingwen, FENG Shaobin, FENG Hong, HAN Junqing. Association between high expression of scavenger receptor class B type I and the prognosis of colon cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 84-89.
[13] WANG Bishi, SHAN Junqi, HOU Qingsheng, GONG Weipeng, ZHU Zhenyu, GUO Hongliang. Effort of CD11b+ / CD66b- phenotype myeloid cells on the progression of colon cancer and liver metastasis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 41-45.
[14] HE Wubin, SU Rongjian. Pulsatilla saponin D combined with sorafenib against hepatocellular carcinoma cell invasion and metastasis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 18-22.
[15] YUE Qianqian, WANG Xinyi, YANG Zhiqiang, JIANG Shu. Quantitative analysis of spectral CT imaging on hypervascular hepatic metastasis and hepatocellular carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 50-55.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd