Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (5): 52-57.doi: 10.6040/j.issn.1671-7554.0.2017.1137

Previous Articles    

Effects of stigma maydis polysaccharides on liver protection and glycometabolism in diabetic mice

ZHANG Zhongyi1, ZHANG Qi1, JIE Yi1, ZHOU Si1, WU Haiyan1, ZANG Ying2, PAN Jingxue3, WU Yuping3, NA Xiaodong4   

  1. 1. Department of Clinical Medicine;
    2. Department of Physiology;
    3. Functional Laboratory of Experimental Center for Basic Medical Teaching;
    4. Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong, China
  • Received:2017-11-13 Published:2022-09-27

PDF (PC)

140

Abstract: Objective To investigate the effects of stigma maydis polysaccharides on glycometabolism and liver protection in diabetic mice. Methods Fifty mice were divided into normal control group(NC group), hyperglycemia control group(HG group), metformin group(MET group), 25 mg/kg polysaccharide derived from stigma maydis group(SMP25 group), and 50 mg/kg polysaccharide derived from stigma maydis group(SMP50 group), ten in each group. The diabetic mice models were prepared by peritoneal injection of alloxan, the fasting plasma glucose(FPG)were measured by a blood glucose tester, the content of liver glycogen in mice were measured by colorimetry, and the liver injury was evaluated by drug-induced liver injury-pathological scroring system(DILI-PSS). Results Compared with 山 东 大 学 学 报 (医 学 版)56卷5期 -张众一,等.玉米须多糖对糖尿病小鼠肝损伤及糖代谢的影响 \=-HG group, FPG value was reduced by 34.3%(P<0.001), the content of glycogen was increased by 321.5% in SMP50 group; compared with MET group, the contents of glycogen were increased(P<0.001), and hepatocyte edema and hepatic steatosis were reduced(P<0.001)in SMP25 and SMP50 groups; the effects of blood sugar control and liver protection in SMP50 group is better than those in SMP25 group(P<0.001). Conclusion Polysaccharides derived from stigma maydis can significantly reduce the FPG concentration, promote the synthesis of glycogen, reduce the liver injury caused by modeling drugs and hyperglycemia in diabetic mice, and is expected to provide a effective adjuvant treatment option for diabetics.

Key words: Polysaccharide derived from stigma maydis, Diabetes, Glycogen, Liver injury

CLC Number: 

  • TS201.1
[1] Lagani V, Koumakis L, Chiarugi F, et al. A systematic review of predictive risk models for diabetes complications based on large scale clinical studies[J]. J Diabetes Complications, 2013, 27(4): 407-413.
[2] Pyram R, Kansara A, Banerji MA, et al. Chronic kidney disease and diabetes[J]. Maturitas, 2012, 71(2): 94-103.
[3] Forbes JM, Cooper ME. Mechanisms of diabetic complications[J]. Physiol Rev, 2013, 93(1): 137-188.
[4] Hazlehurst JM, Woods C, Marjot T, et al. Non-alcoholic fatty liver disease and diabetes[J]. Metabolism, 2016, 65(8): 1096-1108.
[5] Ahmadieh H, Azar ST. Liver disease and diabetes: association, pathophysiology, and management[J]. Diabetes Res Clin Pract, 2014, 104(1): 53-62.
[6] Targher G, Byrne CD. Clinical review: nonalcoholic fatty liver disease: a novel cardiometabolic risk factor for type 2 diabetes and its complications[J]. J Clin Endocrinol Metab, 2013, 98(2): 483-495.
[7] 宋烨,王明彦,宋成武,等.玉米须水煎液对1型、2型糖尿病小鼠降血糖活性的研究[J].时珍国医国药, 2013, 24(11): 2627-2630. SONG Ye, WANG Mingyan, SONG Chengwu, et al. Hypoglycemic effect of corn silk decoction in alloxan-induced type 1 and type 2 diabetic mice[J]. Lishizhen Medicine and Materia Medica Research, 2013, 24(11): 2627-2630.
[8] 朱亮,石海春,罗强,等.玉米须多糖的提取、组成和生物活性研究[J].四川大学学报(自然科学版), 2013, 50(3): 631-637. ZHU Liang, SHI Haichun, LUO Qiang, et al. Purification, composition and biological activity of the polysaccharide isolated from corn silk[J]. Journal of Sichuan University(Natural Science Edition), 2013, 50(3): 631-637.
[9] 陈琛,徐华影.玉米须抗糖尿病活性成分的分离及其作用机制研究[J].中成药, 2015, 37(7): 1492-1497. CHEN Chen, XU Huaying. Separation of anti-diabetic constituents from corn stigma and their mechanism of action[J]. Chinese Traditional Patent Medicine, 2015, 37(7): 1492-1497.
[10] 徐丹鸿,林道鹏.超声波法提取酸浆宿萼多糖条件的优化[J].保鲜与加工, 2015, 15(4): 43-46. XU Danhong, LIN Daopeng. Optimization of ultrasonic extraction of polysaccharides in persistent calyx of physalis alkekengi[J]. Storage and Process, 2015, 15(4): 43-46.
[11] 骆静方,魏巍,乔楠,等.鞣花酸对四氧嘧啶糖尿病小鼠的影响[J].中国糖尿病杂志, 2016, 24(7): 649-651. LUO Jingfang, WEI Wei, QIAO Nan, et al. Effect of ellagic acid on alloxan-induced diabetes in mice[J]. Chin J Diabetes, 2016, 24(7): 649-651.
[12] Garcia-Cortes M, Lucena MI, Pachkoria K, et al. Evaluation of naranjo adverse drug reactions probability scale in causality assessment of druginduced liver injury[J]. Aliment Pharmacol Ther, 2008, 27(9): 780-789.
[13] Penckofer S, Doyle T, Byrn M, et al. State of the science: depression and type 2 diabetes[J]. West J Nurs Res, 2014, 36(9): 1158-1182.
[14] Waterfalli M, Musekiwa A, Engel ME, et al. The prevalence of type 2 diabetes mellitus among older people in Africa: a systematic review study protocol[J]. BMJ Open, 2014, 4(6): e004747. doi: 10.1136/bmjopen-2013-004747.
[15] [No authors listed] Summary of revisions: standards of medical care in diabetes-2018[J]. Diabetes Care, 2018, 41(Suppl 1): S4-S6.
[16] Chang CC, Yuan W, Roan HY, et al. The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity[J]. BMC Complement Altern Med, 2016, 16(1): 432.
[17] Yang J, Li X, Xue Y, et al. Anti-hepatoma activity and mechanism of corn silk polysaccharides in H22 tumor-bearing mice[J]. Int J Biol Macromol, 2014, 64: 276-280.
[18] 宫春宇,郑喜群,陈泽峰,等.玉米须多糖现状研究[J].江苏农业科学, 2016, 44(3): 4-7. GONG Chunyu, ZHENG Xiqun, CHEN Zefeng, et al. Research progress on polysaccharides from corn silk[J].The Jiangsu Agricultural Sciences, 2016, 44(3): 4-7.
[19] Cha JH, Kim SR, Kang HJ, et al. Corn silk extract improves cholesterol metabolism in C57BL/6J mice fed high-fat diets[J]. Nutr Res Pract, 2016, 10(5): 501-506.
[20] Joel Z, Per-Henrik G. Strategies for diabetes management: using newer oral combination therapies early in the disease[J]. Diabetes Ther, 2016, 7(4): 621-639.
[21] Li W, Yuan G, Pan Y, et al. Network pharmacology studies on the bioactive compounds and action mechanisms of natural products for the treatment of diabetes mellitus: a review[J]. Front Pharmacol, 2017, 2(8): 1-9.
[22] Suzuki A, Brunt EM, Kleiner DE, et al. The use of liver biopsyevanluation in discrimination of idiopathic autoimmune hepatitis versus drug-induced liver injury[J]. Hepatology, 2011, 54(3): 931-939.
[23] Ahn JM, Palk YH, Kim SH, et al. Relationship of liver stiffness and controlled attenuation parameter measured by transient elastography with diabetes mellitus in patients with chronic liver disease[J]. J Korean Med Sci, 2014, 29(8): 1113-1119.
[24] Kramer CK, Retnakaran R. Liver enzymes and type 2 diabetes: a complex two-wat relationship[J]. J Diabetes Complications, 2013, 27(4): 301-302.
[25] Garcia-Compean D, Gonzalez-Gonzalez JA, Lavalle-Gonzalez FJ, et al. The treatment of diabetes mellitus of patients with chronic liver disease[J]. Ann Hepatol, 2015, 14(6): 780-788.
[1] QIN Chaoqun, HUANG Bin, YANG Fang, WANG Changming, XIAO Ying, HUANG Hancan, LI Liying, GAO Feng. GSK3β/eEF2K signaling involves in the induced differentiation of lung fibroblasts by regulating autophagy [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 8-15.
[2] LI Tao, YANG Chunlin, DU Tong, LI Heng, WANG Congcong, LI Xiaoli, DUAN Ruisheng,ZHANG Peng. Effects of diabetes mellitus on the subsets and function of NK cells in myasthenia gravis [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 31-36.
[3] TANG Qinlian, ZHANG Yuchao, ZHAO Huichen, MA Xiaoli, YU Minmin, LIU Yuantao. A case report of early-onset diabetes mellitus carrying paired box 4 gene mutation and literature review [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 104-108.
[4] LYU Li, JIANG Lu, CHEN Shihong, ZHUANG Xianghua, SONG Yuwen, WANG Dianhui, AN Wenjuan, LI Qian, PAN Zhe. Related factors of osteoporosis in 210 postmenopausal women with type 2 diabetes mellitus [J]. Journal of Shandong University (Health Sciences), 2021, 59(7): 19-25.
[5] Fengjie ZHENG,Yuwen SONG,Aili SUN,Zhe PAN,Dianhui WANG,Nengjun LOU,Li LYU,Xianghua ZHUANG,Shihong CHEN. Correlation between diabetic peripheral neuropathy and sarcopenia [J]. Journal of Shandong University (Health Sciences), 2021, 59(6): 38-44.
[6] Ping LIU,Yuwen SONG,Ping WANG,Guangwei TIAN,Fengjie ZHENG,Li LYU,Jiaojiao DU,Jing ZHANG,Xianghua ZHUANG,Shihong CHEN. Correlation between vitamin D deficiency and depression in patients with type 2 diabetes mellitus [J]. Journal of Shandong University (Health Sciences), 2021, 59(6): 51-56,102.
[7] XIAO Yang, TAO Yu, WANG Fangyi, LIANG Yuxiu, ZHANG Jin, JI Xiaokang, WANG Zhiping. Association between PM2.5 and PM10 exposure with gestational diabetes mellitus in certain areas of Shandong Province [J]. Journal of Shandong University (Health Sciences), 2021, 59(12): 101-109.
[8] SU Ping, YANG Yachao, YANG Yang, JI Jiadong, DAYIMU Alimu, LI Min, XUE Fuzhong, LIU Yanxun. Prediction models on the onset risks of type 2 diabetes among the health management population [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(6): 82-86.
[9] ZHANG Zhentang, YANG Yang, HAN Fujun, CHEN Xianghua, JI Xiaokang, WANG Yongchao, WANG Shukang, SUN Yuanying, LI Min, CHEN Yafei, WANG Li, XUE Fuzhong, LIU Yanxun. A prediction model of 5-year CVD risks for type 2 diabetic patients: a prospective cohort study among Chinese community population [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(6): 108-113.
[10] LI Shuai, WANG Yalin, SUN Zhongwen, ZHU Meijia. Expression changes and mechanism of Nod-like receptor protein 3 inflammasome in the cerebral microvascular endothelial cells in type 2 diabetes mellitus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 6-11.
[11] ZHANG Yu, HAN Chen, WANG Zhaoxia, WANG Zhaopeng, ZHANG Yueying, ZHOU Shuping, MA Ranran, WANG Hengxiao. Protective effects of Tamarix chinensis Lour on mice with alcoholic liver injury and its mechanism [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(2): 61-67.
[12] XIE Haibin, WU Qunzheng, LIU Shaozhuang, HUANG Xin, CHENG Yugang, HU Sanyuan, ZHANG Guangyong. Role of mitochondria-associated membranes in the improvement of insulin sensitivity in diabetic rats after sleeve gastrectomy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 36-40.
[13] YANG Yang, ZHANG Guang, ZHANG Chengqi, SONG Xinhong, XUE Fuzhong, WANG Ping, WANG Li, LIU Yanxun. A prediction model for type 2 diabetes risks: a cohort study based on health examination [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 69-72.
[14] PENG Li, QIANG Ye, ZHAO Huichen, CHEN Shihong, YAO Weidong, LIU Yuantao. Clinical studies on type 2 diabetic patients treated with sitagliptin [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 60-63.
[15] LIN Dong, GUAN Qingbo. Association of serum testosterone level and non-alcoholic fatty liver disease in male patients with T2DM [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 33-37.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd