柽柳对小鼠酒精性肝损伤的保护作用及机制

doi:10.6040/j.issn.1671-7554.0.2016.995

Abstract

Abstract: Objective To study the protective effects and mechanism of the ethanol extract of Tamarix chinensis Lour on mice with alcoholic liver injury. Methods Alcoholic liver injury was induced in mice through chronic plus binge alcohol feeding. Eighty male C57BL/6J mice were randomly divided into 5 groups: normal control group, model group, positive control group, Tamarix chinensis Lour with low dose group and Tamarix chinensis Lour with high dose group. The body weight, liver weight and liver index of mice were compared between these groups. The liver fat accumulation and liver injury situation were observed by HE staining and oil red O staining. Biochemical markers of hepatic damage such as serum glutamic pyruvic transaminase(ALT), serum triglyceride(TG), heptic glutamic oxaloacetic transaminase(AST), superoxide dismutase(SOD)and malondialdehyde(MDA)were determined in all groups. The protein expression levels of NOD-like receptor family, pyrin domain containing3(NLRP3), apoptosis-associated speck-like protein containing a CARD(ASC), cysteinyl aspartate specific proteinase-1(caspase-1)and proinflammatory cytokines IL-1β were determined by immunohistochemistry method. Results Compared with the model group, the liver index of mice in Tamarix chinensis Lour with low dose and high dose groups decreased significantly(P<0.05), 山东大学学报 (医学版)55卷2期 -张钰,等.柽柳对小鼠酒精性肝损伤的保护作用及机制 \=-hepatic fat accumulation and inflammation alleviated significantly, and the levels of ALT in serum and AST in liver tissues were significantly decreased(P<0.01); MDA content decreased significantly(P<0.05), the activity of SOD increased significantly(P<0.05), the expressions of NLRP3, ASC, caspase-1 and IL-1β in liver was significantly reduced(P<0.05)in Tamarix chinensis Lour with low dose group. Conclusion Tamarix chinensis Lour can prevent alcohol-induced liver injury occurrence and development, whose mechanism may be related to the inhibition of NLRP3-caspase-1-IL-1β signaling.

Key words: NLRP3 Inflammasome, Alcoholic liver injury, Tamarix chinensis Lour, IL-1β

CLC Number:

R967

ZHANG Yu, HAN Chen, WANG Zhaoxia, WANG Zhaopeng, ZHANG Yueying, ZHOU Shuping, MA Ranran, WANG Hengxiao. Protective effects of Tamarix chinensis Lour on mice with alcoholic liver injury and its mechanism[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(2): 61-67.

References

[1] Torruellas C, French SW, Medici V. Diagnosis of alcoholic liver disease[J]. World J Gastroenterol, 2014, 20(33): 11684-11699.
[2] Ding RB, Tian K, Huang LL, et al. Herbal medicines for the prevention of alcoholic liver disease: a review[J]. J Ethnopharmacology, 2012, 144(3): 457-465.
[3] Del Prete A, Scalera A, Iadevaia MD, et al. Herbal products: benefits, limits, and applications in chronic liver disease[J]. Evid Based Complement Alternat Med, 2012: 837939.
[4] Zhao L, Peng XJ, Xia PF, et al. Chemical constituents of Tamarix chinensis[J]. Zhong Yao Cai, 2014, 37(1): 61-63.
[5] 廖菁, 邢亚超, 李宁, 等. 柽柳属植物的化学成分和药理活性研究进展[J]. 现代药物与临床, 2012, 27(4): 404-408. LIAO Jing, XING Yachao, LI Ning, et al. Advances in studies on chemical constituents in plants of Tamarix L. and their pharmacological activities[J]. Drugs & Clinic, 2012, 27(4): 404-408.
[6] Abouzid S, Sleem A. Hepatoprotective and antioxidant activities of Tamarix nilotica flowers[J]. Pharm Biol, 2011, 49(4): 392-395.
[7] Assiri AMA. Protective effect of Tamarix amplexicaulis seeds against hepatotoxicity induced by carbon tetrachloride, paracetamol, or D-galactosamine[J]. J Saudi Chem Soc, 2006, 10(3): 437-446.
[8] Gross O, Thomas CJ, Guarda G, et al. The inflammasome: an integrated view[J]. Immunol Rev, 2011, 243(1): 136-151.
[9] Cui K, Yan G, Xu C, et al. Invariant NKT cells promote alcohol-induced steatohepatitis through interleukin-1beta in mice[J]. J Hepatol, 2015, 62(6): 1311-1318.
[10] DeSantis DA, Ko CW, Liu Y, et al. Alcohol-induced liver injury is modulated by nlrp3 and nlrc4 inflammasomes in mice[J]. Mediators of Inflamm, 2013: 751374.
[11] Henao-Mejia J, Elinav E, Jin C, et al. Inflammasome-mediated dysbiosis regulates progression of NAFLD and obesity[J]. Nature, 2012, 482(7384): 179-185.
[12] 毛竹君, 齐方洲, 熊耀康, 等. 多枝柽柳抗炎、镇痛、抗氧化作用活性部位的筛选[J]. 中国中医药科技, 2013, 20(4): 363-364.
[13] Abouzid S, Sleem A. Hepatoprotective and antioxidant activities of Tamarix nilotica flowers[J]. Pharm Biol, 2011, 49(4): 392-395.
[14] 陈柳生,梁晓欣,蔡自由,等.柽柳的化学成分研究[J]. 中草药, 2014, 45(13): 1829-1833. CHEN Liusheng, LIANG Xiaoxin, CAI Ziyou, et al. Chemical constituents from Tamarix chinensis[J]. Chinese Traditional and Herbal Drugs, 2014, 45(13): 1829-1833.
[15] Ki SH, Park O, Zheng M, et al. Interleukin-22 treatment ameliorates alcoholic liver injury in a murine model of chronic-binge ethanol feeding: role of signal transducer and activator of tran-scription 3[J]. Hepatology, 2010, 52(4): 1291-1300.
[16] 韩琛,王朝霞,贾青,等.蝎毒多肽提取物调控肝脏移植瘤中自然杀伤细胞浸润的机制研究[J]. 中国免疫学杂志, 2016, 32(3): 390-396. HAN Chen, WANG ZhaoXia, JIA Qing, et al. Regulatory mechanism of PESV on tumor-infiltrating natural killer cells in liver orthotopic transplantation tumor[J]. Chin J Immun, 2016, 32(3): 390-396.
[17] Bertola A, Mathews S, Ki SH, et al. Mouse model of chronic and binge ethanol feeding(the NIAAA model)[J]. Nat Protoc, 2013, 8(3): 627-637.
[18] Zhu H, Jia Z, Misra H. Oxidative stress and redox signaling mechanisms of alcoholic liver disease: updated experimental and clinical evidence[J]. J Dig Dis, 2012, 13(3): 133-142.
[19] Galicia-Moreno M, Gutierrez-Reyes G. The role of oxidative stress in the development of alcoholic liver disease[J]. Rev Gastroenterol Mex, 2014, 79(2): 135-144.
[20] Gao L, Zhou Y, Zhong W, et al. Caveolin-1 is essential for protecting against binge drinking-induced liver damage through inhibiting reactive nitrogen species[J]. Hepatology, 2014, 60(2): 687-699.
[21] Zintzaras E, Stefanidis I, Santos M, et al. Do alcohol-metabolizing enzyme gene polymorphisms increase the risk of alcoholism and alcoholic liver disease[J]. Hepatology, 2006, 43(2): 352-361.
[22] Zhou JY, Jiang ZA, Zhao CY, et al. Long term binge and escalating ethanol exposure causes necroinflammation and fibrosis in rat liver[J]. Alcohol Clin Exp Res, 2013, 37(2): 213-222.
[23] Ozaki E, Campbell M, Doyle SL. Targeting the NLRP3 inflammasome in chronic inflammatory diseases: current perspectives[J]. J Inflamm Res, 2015, 8: 15-27.
[24] Bauernfeind FG, Horvath G, Stutz A, et al. Cutting edge: NF-kappaB activating pattern recognition and cytokine receptors license NLRP3 inflammasome 84 activation by regulating NLRP3 expression[J]. J Immunol, 2009, 183(2): 787-791.
[25] Wree A, Eguchi A, McGeough MD. NLRP3 inflammasome activation results in hepatocytepyroptosis, liver inflammation and fibrosis[J]. Hepatology, 2014, 59(3): 898-910.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Protective effects of Tamarix chinensis Lour on mice with alcoholic liver injury and its mechanism

PDF (PC)

Abstract

Cite this article

share this article

References

Related Articles 2

Metrics

Comments

Recommended 0

[1]	ZHANG Luan, CHEN Ou, LUAN Yun, ZHU Xiaobo, CHEN Yuan, WANG Yibiao. Effects of gemigliptin on the therapeutic effect and inflammatory factors of monocrotaline-induced pulmonary arterial hypertension in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 19-22.
[2]	LIU Zhengmei, JIANG qiong, ZHOU Jianjiang, GUAN Zhizhong, ZHAO Yan, XIONG Lin, XIE Yuan. Effect of Helicobacter pylori infection on NLRP3 inflammasome activation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 9-13.