Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (5): 8-15.doi: 10.6040/j.issn.1671-7554.0.2021.0878

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GSK3β/eEF2K signaling involves in the induced differentiation of lung fibroblasts by regulating autophagy

QIN Chaoqun, HUANG Bin, YANG Fang, WANG Changming, XIAO Ying, HUANG Hancan, LI Liying, GAO Feng   

  1. Department of Respiratory and Critical Care Medicine, Guilin Peoples Hospital, Guilin 541002, Guangxi, China
  • Published:2022-06-01

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Abstract: Objective To explore the expression of glycogen syntheses kinases 3β(GSK3β)/eukaryotic elongation factor 2 kinases(eEF2K)during the conversion of lung fibroblasts into myofibroblasts, and to investigate its effects on cellular fibrosis and autophagy. Methods L-929 cells(control group)were cultured under appropriate conditions for 48 h, and transformed into myofibroblasts(TGF-β1 group)by 5ng/mL TGF-β1. Myofibroblasts were transfected with empty plasmid, si-eEF2K and si-GSK3β. The protein expressions of p-eEF2K and α-SMA were detected with immunofluorescence staining after 48 hours of culture, and the protein expressions of eEF2K, p-eEF2K, GSK3β, P62 and LC3-Ⅱ/Ⅰ were detected with Western blotting. Results Compared with the control group, the TGF-β1 group had increased expression of p-eEF2K, GSK3β and α-SMA(P<0.05). After transfection with si-eEF2K and si-GSK3β, the expressions of p-eEF2K and α-SMA decreased, while the expressions of P62 and LC3-Ⅱ/Ⅰ increased(P<0.05). Conclusion GSK3β/eEF2K signalling may promote the phenotypic transformation of lung fibroblasts to myofibroblasts by reducing autophagic activity and facilitating the process of cellular fibrosis.

Key words: L-929 cells, Fibroblasts, Myofiber cells, Glycogen syntheses kinases 3β/eukaryotic Elongation factor 2 kinases, siRNA transfection

CLC Number: 

  • R563.9
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