腺苷酸依赖的蛋白激酶在子宫腺肌症中的表达及其作用

doi:10.6040/j.issn.1671-7554.0.2018.1460

摘要/Abstract

摘要： 目的通过检测腺苷酸依赖的蛋白激酶(AMPK)在子宫腺肌症患者在位子宫内膜组织中的表达和定位,探讨AMPK在子宫腺肌症发病中的作用。方法免疫组织化学染色法检测子宫腺肌症患者在位子宫内膜组织(20例)和对照组正常子宫内膜(20例)中AMPK的表达情况;Real-time PCR检测子宫腺肌症患者在位子宫内膜组织和对照组正常子宫内膜中AMPK、M2型丙酮酸激酶(PKM2)mRNA表达水平;Western blotting检测子宫腺肌症患者在位子宫内膜组织和对照组正常子宫内膜中AMPK、PKM2蛋白表达水平。结果 AMPK主要在子宫腺肌症患者在位子宫内膜组织和对照组正常子宫内膜中的腺上皮细胞质中表达,免疫组化结果显示子宫腺肌症患者在位子宫内膜腺上皮细胞中AMPK表达在分泌期和增殖期均高于正常子宫内膜(P<0.001),而对照组、实验组相应的增殖期和分泌期中AMPK表达差异无统计学意义(P均>0.05)。子宫腺肌症患者在位内膜组织中AMPK、PKM2 mRNA表达水平和蛋白表达水平均高于正常子宫内膜,差异有统计学意义(P<0.001),而对照组、实验组相应的增殖期和分泌期中AMPK、PKM2 mRNA表达水平和蛋白表达水平差异无统计学意义(P>0.05)。结论 AMPK可能通过促进糖酵解代谢在子宫腺肌症的发生发展中发挥作用。

关键词: 子宫腺肌症, 子宫内膜, 腺苷酸依赖的蛋白激酶, M2型丙酮酸激酶, 信号通路

Abstract: Objective To explore the role of adenosine monophosphate dependent protein kinase(AMPK)in the pathogenesis of adenomyosis by investigating its expression and localization in the eutopic endometrial tissues in adenomyosis. Methods The localizations of AMPK in eutopic adenomyosis endometrium(20 cases)and normal endometrium(20 cases)were detected with immunohistochemistry. The mRNA and protein expressions of AMPK and M2 pyruvate kinase(PKM2)were determined with Real-time PCR and Western blotting, respectively. Results AMPK was mainly expressed in the glandular epithelial cytoplasm in normal endometrium and eutopic adenomyosis endometrium. Immunohistochemistry showed that AMPK expression was higher in eutopic adenomyosis endometrium than in normal endometrium either in the secretory or proliferative phases(P<0.001), while there were no statistical differences in the expression of AMPK in the proliferative phase and secretory phase of each group(all P>0.05). The mRNA and protein expressions of AMPK and PKM2 were higher in eutopic adenomyosis endometrium than in normal endometrium(P<0.001), while there were no statistical differences in the mRNA and protein expressions of adenosine monophosphate and PKM2 山东大学学报 (医学版)57卷2期 -黄毓菲,等.腺苷酸依赖的蛋白激酶在子宫腺肌症中的表达及其作用 \=-in the proliferative phase and secretory phase of each group(all P>0.05). Conclusion AMPK promotes glycolysis and plays an important role in the genesis and development of adenomyosis.

Key words: Adenomyosis, Endometrium, Adenosine monophosphate-dependent protein kinase, M2 pyruvate kinase, Signaling pathway

中图分类号:

R711.71

黄毓菲,杨阳,邵倩倩,邓晓惠,晁岚. 腺苷酸依赖的蛋白激酶在子宫腺肌症中的表达及其作用[J]. 山东大学学报 (医学版), 2019, 57(2): 93-98.

HUANG Yufei, YANG Yang, SHAO Qianqian, DENG Xiaohui, CHAO Lan. Expression and role of adenosine monophosphate dependent protein kinase in adenomyosis[J]. Journal of Shandong University (Health Sciences), 2019, 57(2): 93-98.

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