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山东大学学报 (医学版) ›› 2022, Vol. 60 ›› Issue (2): 27-31.doi: 10.6040/j.issn.1671-7554.0.2021.1307

• 临床医学 • 上一篇    下一篇

聚腺苷酸二磷酸核糖聚合酶-1在子宫内膜异位症中的表达及意义

林雪艳1,张灿灿2,田民乐3,田永杰1,4   

  1. 1. 山东第一医科大学附属省立医院妇产科, 山东 济南 250021;2. 山东大学附属山东省妇幼保健院妇科, 山东 济南 250014;3. 山东第一医科大学基础医学院, 山东 济南 250118;4. 山东大学附属省立医院妇产科, 山东 济南 250021
  • 发布日期:2022-01-25
  • 通讯作者: 田永杰. E-mail:tianyongjie@sdu.edu.cn
  • 基金资助:
    山东省自然科学基金(ZR2019MH129);中华预防医学会科研资金支持项目(201815072)

Expression and significance of poly(ADP-ribose) polymerase-1 in endometriosis

LIN Xueyan1, ZHANG Cancan2, TIAN Minle3, TIAN Yongjie1,4   

  1. 1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong Frist Medical University, Jinan 250021, Shandong, China;
    2. Department of Gynecology, Shandong Provincial Maternal and Child Health Care Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250014, Shandong, China;
    3. School of Basic Medicine, Shandong First Medical University, Jinan 250118, Shandong, China;
    4. Department of Obstetrics and Gynecology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong, China
  • Published:2022-01-25

摘要： 目的 检测聚腺苷酸二磷酸核糖聚合酶-1(PARP-1)在子宫内膜异位症患者在位内膜和异位内膜组织中的表达,并探讨PARP-1与E-cadeherin、vimentin和Snail在子宫内膜异位症患者内膜中表达的相关性。 方法 采用免疫组织化学SP法检测PARP-1、E-cadeherin、vimentin和Snail在子宫内膜异位症患者在位内膜、异位内膜、正常子宫在位内膜中的表达。采用Spearman方法分析PARP-1与E-cadeherin、vimentin和Snail之间表达的相关性。 结果 PARP-1、vimentin和Snail在子宫内膜异位症患者在位内膜及异位内膜中的表达比正常子宫在位内膜中的表达增高(P<0.001),E-cadherin在子宫内膜异位症患者在位内膜及异位内膜中的表达较正常子宫在位内膜表达降低(P<0.001)。在子宫内膜异位症患者在位内膜中,PARP-1的表达与E-cadherin呈负相关(r=-0.39),与vimentin和Snail的表达呈正相关(r分别为0.45和0.44);在子宫内膜异位症患者异位内膜中,PARP-1的表达与E-cadherin呈负相关(r=-0.41),与vimentin和Snail的表达呈正相关(r分别为0.48和0.56)。 结论 PARP-1在子宫内膜异位症在位内膜及异位内膜中表达增高,PARP-1可能参与了子宫内膜异位症的上皮间质转化过程。

关键词: 聚腺苷酸二磷酸核糖聚合酶-1, 子宫内膜异位症, 上皮-间质转化, E-cadherin蛋白, Snail蛋白

Abstract: Objective To detect the expression of poly(ADP-ribose)polymerase-1(PARP-1)in eutopic and ectopic endometria of women with endometriosis, and to explore the correlation among PARP-1, E-cadherin, vimentin and Snail in eutopic and ectopic endometria of women with endometriosis. Methods The expressions of PARP-1, E-cadherin, Vimentin and Snail were detected with immunohistochemistry in eutopic and ectopic endometria of women with endometriosis, and in eutopic endometria of women without endometriosis. The correlation among their expressions was determined with Spearman analysis. Results The expressions of PARP-1, vimentin and Snail significantly increased in eutopic and ectopic endometria of women with endometriosis than in endometria of women without endometriosis(P<0.001), while the expression of E-cadherin significantly decreased(P<0.001). In eutopic endometria of endometriosis, the expression of PARP-1 was negatively correlated with E-cadherin(r=-0.39), but positively correlated with the expressions of vimentin and Snail(r=0.45 and 0.44, respectively); in ectopic endometria of endometriosis, the expression of PARP-1 was negatively correlated with E-cadherin(r=-0.41), but positively correlated with the expressions of vimentin and Snail(r=0.48 and 0.56, respectively). Conclusion PARP-1 is highly expressed in eutopic and ectopic endometria of women with endometriosis, PARP-1 may be involved in the epithelial mesenchymal transformation of endometriosis.

Key words: Poly(ADP-ribose)polymerase-1, Endometriosis, Epithelial mesenchymal transformation, E-cadherin protein, Snail protein

中图分类号: 

  • R711.71
[1] Zhu TH, Zhang XM. Research progress on the role of epithelial-mesenchymal transition in pathogenesis of endometriosis [J]. Zhejiang Da Xue Xue Bao Yi Xue Ban, 2016, 45(4): 439-445.
[2] Yang YM, Yang WX. Epithelial-to-mesenchymal transition in the development of endometriosis [J]. Oncotarget, 2017, 8(25): 41679-41689.
[3] Lutz Konrad, Raimund Dietze, Muhammad A Riaz, et al. Epithelial-mesenchymal transition in endometriosis-when does it happen? [J]. Clin Med, 2020, 9(6): 1915. doi: 10.3390/jcm9061915.
[4] Su Shan, Lin XY, Ding N, et al. Effects of PARP-1 inhibitor and ERK inhibitor on epithelial mesenchymal transitions of the ovarian cancer SKOV3 cells [J]. Pharmacol Rep, 2016, 68(6): 1225-1229.
[5] Wei W, Lv SQ, Zhang CC, et al. PARP-1 may be involved in angiogenesis in epithelial ovarian cance r[J]. Oncol Lett, 2016, 12(6): 4561-4567.
[6] Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition [J]. Nat Rev Mol Cell Biol, 2014, 15(3): 178-196.
[7] Biyik I, Kalkan U, Simsek S. The deep infiltrating endometriosis tissue has lower T-cadherin, E-cadherin, progesterone receptor and oestrogen receptor than endometrioma tissue [J]. Taiwan J Obstet Gynecol, 2021, 60(6): 1059-1065.
[8] Batlle E, Sancho E, Francí C, et al.The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells [J]. Nat Cell Biol, 2000, 2(2): 84-89.
[9] Garinet S, Didelot A, Denize T, et al. Clinical assessment of the miR-34, miR-200, ZEB1 and SNAIL EMT regulation hub underlines the differential prognostic value of EMT miRs to drive mesenchymal transition and prognosis in resected NSCLC [J]. Br J Cancer, 2021, 125(11): 1544-1551.
[10] Cano A, Pérez-Moreno MA, Rodrigo I, et al. The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression [J]. Nat Cell Biol, 2000, 2(2): 76-83.
[11] Xi L, Yang J, Wang X, et al. Role of TWIST2, E-cadherin and Vimentin in epithelial ovarian carcinogenesis and prognosis and their interaction in cancer progression [J]. Eur J Gynaecol Oncol, 2016, 37(1): 100-108.
[12] Willipinski-Stapelfeldt B, Riethdorf S, Assmann V, et al. Changes in cytoskeletal protein composition indicative of an epithelial-mesenchymal transition in human micrometastatic and primary breast carcinoma cells [J]. Clin Cancer Res, 2005, 11(22): 8006-8014.
[13] Scotti S, Regidor PA, Schindler AE, et al. Reduced proliferation and cell adhesion in endometriosis [J]. Mol Hum Reprod, 2000, 6(7): 610-617.
[14] Zhu H, Wei M, Xu J, et al. PARP inhibitors in pancreatic cancer: molecular mechanisms and clinical applications [J]. Mol Cancer, 2020,19(1): 49. doi: 10.1186/s12943-020-01167-9.
[15] Wei W, Li Y, Lv SQ, et al. PARP-1 may be involved in angiogenesis in epithelial ovarian cancer [J]. Oncol Lett, 2016, 12(6): 4561-4567.
[16] Bahar E, Kim JY, Kim HS, et al. Establishment of Acquired Cisplatin Resistance in Ovarian Cancer Cell Lines Characterized by Enriched Metastatic Properties with Increased Twist Expression [J]. Int J Mol Sci, 2020, 21(20):7613.
[17] Scutiero G, Iannone P, Bernardi G, et al. Oxidative stress and endometriosis: a systematic review of the literature [J]. Oxid Med Cell Longev, 2017, 2017: 7265238.
[18] 郑玉梅, 彭超, 陆叶, 等. 深部浸润型子宫内膜异位症在盆腔子宫内膜异位症中的发生率及其临床病理特征分析[J]. 中华妇产科杂志, 2020, 55(6): 384-389. ZHENG Yumei, PENG Chao, LU Ye, et al. Incidence of deeply infiltrating endometriosis among 240 cases of pelvic endometriosis and analysis of its clinical and pathological characteristics [J]. Chinese Journal of Obstetrics and Gynecology, 2020, 55(6): 384-389.
[19] Béliard A, Donnez J, Nisolle M, et al. Localization of laminin, fibronectin, E-cadherin, and integrins in endometrium and endometriosis [J]. Fertil Steril, 1997, 67(2): 266-272.
[20] Shaco-Levy R, Sharabi S, Benharroch D, et al. Matrix metalloproteinases 2 and 9, E-cadherin, and beta-catenin expression in endometriosis, low-grade endometrial carcinoma and non-neoplastic eutopic endometrium [J]. Eur J Obstet Gynecol Reprod Biol, 2008, 139(2): 226-232.
[21] Pu H, Horbinski C, Hensley PJ, et al. PARP-1 regulates epithelial-mesenchymal transition(EMT)in prostate tumorigenesis [J]. Carcinogenesis, 2014, 35(11): 2592-2601.
[22] Lin Y, Kang T, Zhou BP. Doxorubicin enhances Snail/LSD1-mediated PTEN suppression in a PARP1-dependent manne r[J]. Cell Cycle, 2014, 13(11): 1708-1716.
[23] McPhee TR, McDonald PC, Oloumi A, et al. Integrin-linked kinase regulates E-cadherin expression through PARP-1 [J]. Dev Dyn, 2008, 237(10): 2737-2747.
[24] Chu S, Xu H, Ferro TJ, et al. Poly(ADP-ribose)polymerase-1 regulates vimentin expression in lung cancer cells [J]. Am J Physiol Lung Cell Mol Physiol, 2007, 293(5): L1127-L1134.
[25] Kumar M, Jaiswal RK, Yadava PK, Singh RP. An assessment of poly(ADP-ribose)polymerase-1 role in normal and cancer cells [J]. Biofactors, 2020, 46(6): 894-905.
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