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Table of Content

      
    16 January 2009
    Volume 47 Issue 01
    Articles
    无栏目
    Effect of mitochondrial transfer on the mitochondrial membrane potential of aging mouse preimplantation embryos
    LIU Yanhe1,2, LIU Kai1, LIAN Fang2, ZHANG Jianwei2, SUN Zhengao2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  6-9. 
    Abstract ( 1074 )   PDF (349KB) ( 220 )   Save
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    To investigate the effect of mitochondrial transfer (MIT) of aging mouse oocytes on the mitochondrial membrane potential (△Ψm) of the subsequent preimplantation embryos. MethodsSixto eightweek old female KM BAI mice were superovulated, and the oocytes retrieved were treated by intracytoplasmic sperm injection (ICSI) and medium blank injection. The subsequent embryos were distributed in group A. Twelve months old female KM BAI mice were superovulated and the oocytes obtained were divided into two subsets. One was treated by ICSI and medium blank injection and the subsequent embryos were included in group B, the other was treated by ICSI and MIT and the subsequent embryos were included in group C. Comparisons were performed on △Ψm among the three groups during the preimplantation stages and on the alterations of △Ψm across the preimplantation stages of each group. ResultsAt the 2cell stage and morula stage, △Ψm of embryos from groups A and C were higher than those from group B (P＜0.01), while at the 4cell stage, 8cell stage and blastocyst stage, △Ψm of embryos from groups A and C were also higher than those from group B (P＜0.05). In all of the three groups, △Ψm of embryos at either the morula stage or blastocyst stage was higher than those at any other cleavage stage (P＜0.05), while there were no significant differences between the morula stage and blastocyst stage and also no significant differences among the cleavage stages. ConclusionThe MIT of aging mouse oocytes can significantly improve the B△Ψm of subsequent preimplantation embryos
    Screening of naturally occurring chemicals by enhancing Neprilysin activity and potential applications in treatment of Alzheimer′s disease
    YANG Lingling1, ZHANG Jing1, CUI Yunyan2, HU Xiaoyan1, KONG Feng1, CUI Xing1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  10-14. 
    Abstract ( 1132 )   PDF (669KB) ( 565 )   Save
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    To obtain potential chemicals useful for chemotherapy of Alzheimer′s disease (AD) by screening naturally occurring compounds which could increase the Neprilysin (NEP) activity. MethodsNeuroblastoma cells were cultured (SKNSH cells) and pcDNA3.1sweAPP was transfected into the SKNSH cells with LipofectamineTM2000, and G418 was used for screening. The stably transfected cells were confirmed with Western blot and enzyme linked immunosobent assay (ELISA). MTT was used to identify the optimal drug concentration and time. The effects of drugs on NEP activity were determined by the NEP peptidase assay, and the Aβ levels were determined by ELISA. ResultsWestern blot results showed that SKNSH cells transfected with pcDNA3.1sweAPP highly expressed APP proteins, and ELISA results showed that Aβ40 and Aβ42 in culture media were significantly increased respectively by 7.26 and 3.27 fold after transfection. NEP peptidase assay showed that tea polyphenols, ginsenoside Rb1 and Rg3 could significantly enhance the NEP activity by 117±2.8%, 131±4.0% and 121±1.6% respectively. ELISA results showed that they could significantly decrease Aβ40 and Aβ42 in culture media of SKNSH cells by 82±0.78% and 85±2.21%, 74±1.61% and 99±3.31% and 83±5.31% and 76±6.74% respectively. ConclusionThe AD model cells were successfully constructed, and tea polyphenols, Ginsenoside Rb1 and Rg3 can effectively decrease Aβ40 and Aβ42 in culture media. Our findings offer potent theoretical supports for application of these drugs in treating AD.

    Effect and possible mechanism of music therapy for concentration of
    serum lipids in psychological stress rabbit models
    ZHAO Li, SUN Ping, YAN Fangfang, LIU Yan, ZHAO Yuxia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  15-18. 
    Abstract ( 1065 )   PDF (397KB) ( 406 )   Save
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    To observe the effects of music therapy for the concentration of serum lipids of psychological stress rabbit models and to investigate its possible mechanism. MethodsForty New Zealand male rabbits were randomly divided into 4 groups: the control group (A), the music group (B), the psychological stress group (C) and the psychological stress plus music group (D). Group B was given music 4 hours each day. Group C was given psychological stress. Group D was given music 4 hours and psychological stress each day. The experiment period was 8 weeks. The concentrations of total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDLc), and neuropeptide Y (NPY) were determined at the beginning and end of the experiment, and HDL/LDL was calculated. At the end of the experiment, abdominal aorta of the rabbits was taken out and dyed with rathonum red O which was used to examine the degree of lipidoses in vessel walls. ResultsThe concentration of TC, TG, LDL, HDL, NPY and HDL/LDL was significantly different between group A and group C (P＜0.05) and between group C and group D (P＜0.05), the quantity of lipidoses in the vessel walls of group D was obviously less than that of group C. ConclusionsMusic therapy can alleviate increased serum lipids induced by chronic psychological stress, and the mechanism might relate to music controlling NPY secretion.
    Expression and purification of carbapenemase OXA72 in pichia pastoris
    HAN Jin1, WU Dawei1, FENG Jinbo2, GENG Zhiying3，　YANG Lei1, LI Qiaorong1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  19-22. 
    Abstract ( 1022 )   PDF (680KB) ( 324 )   Save
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    To amplify and identify the chromosomal DNA of Acinetobacter Baumanii which is the first isolate producing carbapenemase OXA72 in our country and to express and purify OXA72 in Pichia pastoris. MethodsThis study amplified the oxa72 gene by PCR using the extracted genomic DNA of the isolate 40 which was obtained from the clinic as a template. After the amplified gene was cloned into vector pGAPZalphaA, linearized recombinant plasmid oxa72pGAPZalphaA was transformed to Pichia pastoris GS115 by electroporation. Then the positive transformants were screened by colony PCR, western blot analysis and Nitrocefin test. Then we purified OXA72 with a nickel column. ResultsThe DNA sequence amplified by PCR from isolate 40 was identical with blaOXA72 (GenBank accession numbers AY739646). One positive transformant had a positive Nitrocefin test. SDSPAGE showed that the relative molecular weight of the recombined protein was 34?kDa43kDa. Western blotting showed the combined protein could specifically bind to the Histag antibody. The recombinant protein was obtained by purification with 95％ final purity and 40％ recovery rate. ConclusionWe successfully expressed and purified the carbapenemase OXA72 in Pichia pastoris.
    Eating disorder related psychological factors of university students
    NIU Juan, ZHANG Hongjing, DU Juan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  23-26. 
    Abstract ( 1308 )   PDF (265KB) ( 1034 )   Save
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    To explore the correlations between bodyimage, perfectionism and social physique anxiety and eating disorder in university students. Methods400 university students were assessed by the Eating Disorder Inventory (EDI), Multidimensional BodySelf Relations Questionnaire (MBSRQ), Chinese version of Frost'S Multidimensional Perfectionism Scale (FMPS) and Social Physique Anxiety Scale(SPAS). Results①The scores of bodyareas satisfaction were lower in the eating disorder group who had a normal weight than in the other three groups, and the scores of FMPS were higher than them. ②Stepwise linear regression showed that bodyareas satisfaction, overweight preoccupation and appearance orientation were independent predictive variables of eating disorder symptoms. ③Principal components analysis showed that the main factors affecting eating disorder symptoms were fervent dissatisfaction of appearance and morbid pursuit for perfection. ConclusionBodyimage disturbance and perfectionism exorbitance are the key factors of eating disorder symptoms in university students.
    Lung tissue damage and level changes of LDH,TAOC and TP of lavage in rats exposed to DT3
    ZHANG Mingliang1,　HOU Lian2, ZHANG Zhili1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  27-29. 
    Abstract ( 1470 )   PDF (522KB) ( 349 )   Save
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    DT3 which is widely used in the aerospace field as a closedcorrectposecontrol propellant, has become the focus of concern because of its safety, especially in respect to its toxicology. To explore DT3 on the toxicology of lungs and its mechanism. MethodsSPF Wistar male rats were given the drug by bronchial injection 14 consecutive days. ResultsThe levels of LDH, TAOC and TP in the bronchalveolar lavage were determined through ELISA, the level of LDH was significantly increased and TAOC significantly decreased (P＜0.05) at a high dose, while no statistical significance of the TP concentration was found in all groups. Also, tail DNA intensity and tail moment were increased in a dosedependent manner in lung tissue cells as determined by fluoroscope through single cell gel electrophoresis. ConclusionDT3 might result in membrane damage by producing lung inflammation. Oxidant damage is not a contributing factor in toxicity at a low dose. The possibility that DT3 induces DNA damage could not be ruled out.
    Expression of DNA methyltransferase 1（DNMT1）in lung adenocarcinoma and its prognosis significance
    YANG Xi1, GAO Peng2, XUE Yuwen1, WANG Yan2, LIU Wenjun2, ZHANG Xiaofang2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  30-33. 
    Abstract ( 1133 )   PDF (637KB) ( 276 )   Save
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    To explore the role of protein expression of DNA methyltransferase 1 (DNMT1) in lung adenocarcinoma carcinogenesis and prognosis. MethodsDNMT1 protein expression levels in 95 tumor specimens and adjacent normal tissues were determined by immunohistochemistry. Comparisons of the survival rate and recurrence between positive and negative DNMT1 were conducted with SPSS13.0 software. ResultsNone of the 95 noncancerous tissues exhibited DNMT1 immunoreactivity, whereas 48 (50.5%) of the 95 lung adenocarcinoma cancerous tissues were DNMT1positive. There was a significant correlation between DNMT1 overexpression and gender (P=0.018), which was much more obvious in females. No correlation was found between DNMT1 expression and patient age, smoking, lymph node metastasis, tumor types and clinical stages (P＞0.05). In addition, general survival rate， 2year survival rate and diseasefree rate in patients with DNMT1positive were 31.91%, 65.07% and 48.65% respectively, while in patients with DNMT1negative they were 62.88%，　83.01% and 26.85%. DNMT1 protein overexpression served as a marker of poor prognosis by KaplanMeier and LogRank analysis(P＜0.05). ConclusionDNMT1 overexpression is an early event of lung adenocarcinoma carcinogenesis and plays a significant role in the development of lung adenocarcinoma. It may serve as a molecule marker to detect lung adenocacinoma in an early stage and an indicator of unfavourable prognosis.
    Kinetogenic effects of serum with semen raphani
    on colon smooth muscle in rats
    SHI Yanting1, SI Chunfeng2, LIU Bin2, LIU Chuanyong1, GUO Jianqiang2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  34-37. 
    Abstract ( 1133 )   PDF (554KB) ( 406 )   Save
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    To study the kinetogenic effects of serum with semen Raphani on colon smooth muscle strips of rats. MethodsSerum containing Chinese materia medica was made based on standard methods. The contractile response of the colonic smooth muscle strips to serum with Chinese materia medica (10％, 50％,100％concentration)and other medicines (control serum and 1×10-3mol/L ACh) were observed. ResultsSerum of Semen Raphani can have kinetogenic effects on transverse and longitudinal smooth muscle strips at different levels (P<0.05). ConclusionSerum with Semen Raphani can produce a notable kinetogenic effect on colonic smooth muscle strips in rats.
    Osteopontin promotes the invasiveness of human ovarian
    carcinoma cell line SKOV3: an experimental study
    LI Huihui1, ZHANG Linfan2, GU Shuang3, ZHANG Xiangning1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  38-41. 
    Abstract ( 992 )   PDF (483KB) ( 183 )   Save
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    To investigate the expression of Osteopontin (OPN) and explore its effect on the invasiveness of human ovarian carcinoma cell line SKOV3. MethodsHuman ovarian carcinoma SKOV3 cells were transfected with plasmid pcDNA 3.1(-)/OPN and cells transfected with a mock plasmid served as controls. RTPCR and Westernblot were used to determine the expressions of OPN mRNA and proteins. Cell invasiveness was measured by the matrigel invasion assay. ResultsExpressions of OPN mRNA and protein were significantly increased (P<0.01) after transfection. Migrating numbers of SKOV3 cells transfected with pcDNA 3.1(-)/OPN were also significantly increased (P<0.05) as compared with the control group. ConclusionOPN play an important role in the metabasis of ovarian cancer.
    Changes of nitric oxide and relationship between nitric oxide and renal injury in obese rats
    LIANG Jun1, SUN Shuzhen2, LI Qian2, CHEN Yao2,　WANG Yi2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  42-44. 
    Abstract ( 1177 )   PDF (342KB) ( 517 )   Save
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    To explore the changes of nitric oxide and its role in renal injury in obese rats. MethodsSixteen healthy male Wistar rats were randomly divided into the control(n=8) and obese group(n=8) which were respectively given an ordinary diet and a high fat diet. After 20 weeks, all rats were killed. The Lee index, left renal index, the content of cholesterol total(TCH), triglyceride(TG), low density lipoprotein cholesterol(LDLC) , high density lipoprotein cholesterol(HDLC) and urine protein quantitation in 24 hours(UPE) and creatinine clearance(Ccr) were detected. Nitric oxide (NO) content in blood , renal tissue and urinary nitrite+nitrate excretion (UNOxV) were acquired by a nitrate disoxidation method. Histomorphology of kidneys was examined by an optical microscope, and the glomerulum section area was determined by image analysator. ResultsIn the obese group, the index of Lee, left renal index, cholesterol total(TCH), triglyceride (TG), low density lipoprotein cholesterol(LDLC), urine protein quantitation in 24 hours and creatinine clearance(Ccr) were significantly higher (P<0.01), while high density lipoprotein cholesterol(HDLC), nitric oxide(NO) content in blood, renal tissue and urinary nitrite+nitrate excretion (UNOxV) were lower(P<0.01). In the obese group, mesangial cell proliferation, Bowman′s space proprius adhesion, interstitial arteriole endothelial hyperplasia, hyalinosis and glomerular average section area were obviously increased (P<0.01). Relative analysis demonstrated that the NO contents of the serum and renal tissue were negatively correlated with TG, TCH, LDLC, UPE, Ccr and glomerulum section area（r=-0.869?4,-0.788?1; -0.795?4,-0.783?5; -0.918?2,-0.873?0; -0.894?0, -0.903?5; -0.612?2, -0.553?5; -0.641?1, -0.599?6）,　while positively correlated with HDLC and UNOxV（r=0.934?9,0.930?7; 0.855?7,0.822?1）. ConclusionThere were evident functional disturbances of blood vessel endothelium and capillary vessel endothelium and renal damage in the obese group. The former is relevant to the conspicuous lipid metabolism disorder, and the latter is relevant to insufficient NO synthesis. As a noninvasive detection indicator, UNOxV can indirectly reflect NO synthesis in obese rats.

    Effect of survivin antisense oligonucleotide antisense transfection on growth and sensitivity to vinorelbine in the human breast cancer cell MCF7
    HU Yu, LI Li, LI Lizhen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  45-49. 
    Abstract ( 1137 )   PDF (521KB) ( 348 )   Save
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    To explore the effects of survivin antisense oligonucleotide(ASODN) on apoptosis, growth and sensitivity to vinorelbine in the human breast cancer cell MCF7. MethodsSurvivinASODN was transfected into the MCF7 cells mediated by the Lip reagent. Expression of survivin mRNA was determined by RTPCR and of survivin protein by Western blot. Apoptosis rate and cell cycle changes in antisense oligonucleotide(ASODN) transfected cells were detected by FCM. Cell proliferation and chemosensitivity to vinorelbine in MCF7 were determined by the MTT assay. The effect of survivin antisense oligonucleotide(ASODN) in inducing apoptosis of MCF7 cells was observed under an electron microscope. ResultsSurvivin ASODNLip compound efficiently downregulated the mRNA and protein expressions of survivin in MCF7 cell lines in a concentrationdependent manner. The cell cycle was arrested at the G2/M phase， the cell apoptosis rate was increased obviously(P＜0.05), and growth inhibition was evidently increased in antisense oligonucleotide(ASODN) transfected cells（P＜0.05）. Terminal apoptosis changes were observed under an electron microscope after antisense oligonucleotide(ASODN) transfection. The 50% inhibitory concentration(IC50) of vinorelbine was significantly lower for 600?nmol/L antisense oligonucleotide(ASODN) transfected MCF7 cells than for control cells ［(3.7±0.42)μg/mL ConclusionSurvivin

    Correlation between acoustic densitometry and myocardial fibrosis and effect of drug intervention on them
    WANG Jing1， DU Naili2， ZHAO Zhuo2， HAO Enkui2, XIANG Youzhang3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  50-55. 
    Abstract ( 1067 )   PDF (741KB) ( 267 )   Save
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    To evaluate the quantitative acoustic densitometry (AD) technique in myocardial fibrosis diagnosis and to explore the effects of benazepril and valsartan on myocardial fibrosis. MethodsSixty tenweekold male spontaneously hypertensive rats (SHRs) were randomly assigned to the control group(SHRc group), the benazepril group(SHRb group), the valsartan group(SHRv group) and the combination group(SHRb+v group), which were subjected to an intragastric administration for ten weeks. Fifteen male agematched WistarKyoto (WKY) rats served as the control group. The integrated backscatter (IBS) and cyclic variation of the integrated backscatter (CVIB) were assessed with the AD technique. The cardiac collagen fraction(CVF) and perivascular circumferential area(PVCA) were determined by Masson staining. Myocardial hyproconcentration(HC) were measured by a spectrophotometric method. Also, systolic blood pressure(SBP), left ventricular mass (LVM) and left ventricular mass index(LVMI) were observed. Correlation analysis among the above indexes was analyzed. ResultsCompared with the WKY group, levels of SBP, LVM, LVMI, HC, CVF, PVCA and IBS were all significantly increased in the SHRc group(P<0.01), while CVIB was significantly decreased(P<0.01), and the indexes were substantially ameliorated in the three treated SHR groups(P<0.05, P<0.01). Except for SBP, the combination group showed a more pronounced amelioration than the other two monotherapy groups (P<0.05, P<0.01). An intimately positive correlation was found between HC and CVF(r=0.921, P<0.01)　or PVCA　(r=0.885, P<0.01). Normalized values of integrated backscatter at the interventricular septum and left ventricular posterior wall were positively related to CVF　(r=0.731, 0.756，P<0.01)　and PVCA　(r=0.776, 0.788，P<0.01), while both CVIBs were inversely related to CVF（r=-0.642,-0.716，P<0.01）and PVCA　（r=-0.720, -0.755, P<0.01）. ConclusionMyocardial ultrasonic backscatter parameters are closely correlated with the alteration of myocardial collagen contents. Quantitative myocardial acoustic densitometry is a reliable technique for detecting myocardial fibrosis and evaluating its severity. Benazepril and valsartan decrease the blood pressure and can reverse an early myocardial fibrosis in SHRs. Benazepril in combination with valsartan is more effective in ameliorating myocardial fibrosis than either one alone.

    A genomic screen on chromosome 3 in coronary heart disease
    in Shandong Province
    XIE Yi1, GUO Yuan1, CHEN Gang2, LI Jifu1, ZHU Haining2,　WEI Ran2, WANG Bo2, LUAN Meng2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  56-60. 
    Abstract ( 1429 )   PDF (980KB) ( 299 )   Save
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    A screen on chromosome 3 in coronary heart disease (CHD) patients and normal controls from Shandong Province was performed to find the genetic loci associated with CHD. MethodsA total of 23 microsatellite markers on chromosome 3 spaced at approximate 10 cM were used in screening of two separated DNA pool samples consisting of 156 CHD cases and 450 normal controls. Statistic analysis was performed by CLUMP software to compare the difference in allele frequency of each locus between the two pooled samples. ResultsSignificant statistic differences were found at D3S1278 and D3S1300 loci between allele frequencies of patients and controls (P<0.001). ConclusionTwo regions on chromosome 3 relate to CHD in Shandong Province and the susceptibility genes surrounding these regions need to be further studied.
    Correlation of TRAIL expression with prognosis of bladder transitional cell carcinoma
    ZHEN Hongtao1,　ZHANG Huaiqiang1,　XU Fuyu2,　et al
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  61-63. 
    Abstract ( 1078 )   PDF (427KB) ( 319 )   Save
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    To determine expression of tumor necrosis factorrelated apoptosisinducing ligand(TRAIL) in bladder transitional cell carcinoma(BTCC) and normal bladder tissues and to explore the correlation among TRAIL and clinicopathological parameters and prognosis of BTCC. MethodsTRAIL protein expression was determined by immunohistochemistry assay (Elivision). ResultsTRAIL negative expression was shown in 45(59.21%) of the 76 cases of BTCC, and it was higher in poorly differentiated(G2, G3) tumors than in well differentiated (G1) tumors (68.63% vs 40.00%, P＜0.05), and also it was significantly higher in multiple tumors than in single tumors (75.86 % vs 48.94%, P＜0.05). Survival curves determined by the KaplanMeier method and logrank test analysis demonstrated that recurrence free survival rates in the group of negative expression of TRAIL were lower than those in the group of positive expression (33.65 months vs 46.49 months, P＜0.05). ConclusionExpression of TRAIL has a negative correlation with different tumor numbers, grades and recurrence of BTCC; therefore, it can be used as a promising marker for prognosis.
    Effect of GPⅡb/Ⅲa, PSelectin and vWF in children with HenochSchonlein purpura
    ZHANG Hongxia1, LIU Xuemei2, LIU Fange1, LIN Aiwei2，　DUAN Chunhong2，　YANG Xiaomei2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  64-67. 
    Abstract ( 1242 )   PDF (280KB) ( 291 )   Save
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    To investigate the role of platelet membrane glycoproteinⅡb/Ⅲa, Pselectin and von Willebrane Factor in children with HenochSchonlein purpura (HSP), and to investigate the pathological role of them in HSP. MethodsThe concentrations of CD41a/CD61 and CD62P were determined by flow cytometry and the level of vWF by an enzymelinked immunosorbent assay(ELISA) method in 44 patients with acute HSP, 40 patients in the recovery phase and 18 healthy controls. ResultsThe plasma levels of CD41a/CD61, CD62P and vWF in the acute phase were significantly higher than those in the recovery phase, were higher than those in healthy controls(P<0.01), and also were higher in the renal damage group than in the nonrenal damage group(P<0.01). ConclusionActivation of platelet and damage of vascular endothelium play important roles in the pathological mechanism of HSP. Levels of CD41a/CD61, CD62P and vWF are helpful in finding the disturbance of blood coagulation，and provide a basis for anticoagulant treatments.
    Dual source CT in detection of coronary artery lesions in
    children with kawasaki disease
    ZHAO Lijian1， HAN Bo1， WANG Ximing2， CHAO Baoting2，
    ZHANG Jianjun1， ZHUANG Jianxin1， ZHANG Yi1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  68-71. 
    Abstract ( 1104 )   PDF (531KB) ( 426 )   Save
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    To evaluate the value of dual source CT(DSCT) in diagnosis of coronary artery lesions due to kawasaki disease in children. MethodsFifteen children with known kawasaki disease and coronary artery lesions confirmed by echocardiography underwent coronary artery angiography by DSCT. The number, location, shape and size of each coronary artery aneurysm was recorded and compared with those determined by echocardiography. ResultsThere were 8 males and 7 females with a mean age of 3 years and 2 months (range 2 months to 11 years). A total of 43 coronary artery aneurysms were found by DSCT, and the maximum diameter of which was 1.55cm (mean 0.65±0.47cm). 15 aneurysms were small in size, 20 aneurysms were medium in size and 8 were giant aneurysms. Among the aneurysms in the left coronary artery, 9 were located in the left main trunk, 4 were located in the proximal segment of the left anterior descending branch(LAD), 5 were located in the medium segment of the LAD, and 3 were located in the left circumflex branch. 22 coronary artery aneurysms were located in the right coronary artery, including 9 in the proximal segment, 7 in the medium segment and 6 in the distal segment. 3 stenotic segments and 2 thrombus were additionally found. 6 aneurysms located in the medium or distal segments, 3 stenotic and 2 thrombus were not found by echocardiography. ConclusionDSCT can clearly visualize coronary artery lesions due to kawasaki disease in children, especially in detection of the stenotic segments and thrombosis.
    Correlation between galectin3 and lymphatic metastasis and
    pleural metastasis in lung adenocarcinoma
    LONG Chengfeng， LIN Dianjie， CUI Jiyun， WAN Yunyan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  72-75. 
    Abstract ( 1318 )   PDF (355KB) ( 272 )   Save
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    To investigate the correlation between galectin3 and lymphatic metastasis and pleural metastasis in adenocarcinoma of the lung. MethodsImmunohistochemistry was used to determine expression of galectin3 in 63 primary lung adenocarcinomas specimens and 34 adenocarcinomas metastatic of pleural specimens. ResultsThe patterns of galectin3 expression were cytoplasmic and nuclear. All cases of nuclear galectin3 also showed a cytoplasmic immunoreactivity. In primary tumors the positive galectin3 expression was found in 92.1% of the cytoplasm and 31.7% of the nucleus. Only the nuclear location of galectin3 was associated with lymphatic metastasis　(P=0.03)　and clinical stages(TNM stages)(P＜0.05)． In pleural secondary adenocarcinomas the positive expression rate of galectin3 in the cytoplasm and nucleus was 97.1% and 85.3% respectively, and the nuclear staining was significantly higher than the primary tumour(P＜0.05). ConclusionNuclear expression of galectin 3 can enhance the invasive and metastatic potential of lung adenocarcinomas and can be used as a biological marker of lymph node and/or pleural metastasis.
    Effect of hypertonic saline/hetastarch solution on intracranial
    pressure in patients with intracranial tumors
    SUN Baozhu1，　HOU Lili2，　ZHANG Jianmin1，　FAN Lixia1, WANG Xinyu3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  76-78. 
    Abstract ( 1300 )   PDF (269KB) ( 334 )   Save
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    To explore the effect of hypertonic saline/hetastarch solution on intracranial pressure in patients undergoing surgery for intracranial tumours. MethodsTwentysix ASA Ⅰ or Ⅱ patients, aged 2871 years and 5089?kg in weight undergoing intracranial tumor removal were randomly divided into two groups (13 in each ):groupⅠwith hypertonic saline/hetastarch solution(HSS40) and group Ⅱ with 20% mannitol (M). All patients were intramuscularly premedicated with 0.1g phenobarbital and 0.5?mg atropine. Under local anesthesia, an epidural catheter (17?G) was inserted into the subarachnoid space at the L34 interspace for measurement of cerebral spinal fluid (CSF) pressure. Anesthesia was induced with 3?μg/kg fentanyl, 4?mg midazolam, 0.3?mg/kg etomidate and 0.1?mg/kg vecuronium. Then patients were mechanically ventilated (VT=10?mL/kg,RR=12?bpm,I∶E=1∶2,FiO2=100%). PETCO2 was maintained 3035?mmHg by adjusting the tidal volume and respiratory rate. Anesthesia was maintained with inhalation of 1.5% isoflurane. The left radial artery was cannulated for BP monitoring and blood sampling. The right internal jugular vein was cannulated for CVP monitoring. HSS40 200?mL or 20% mannitol 250?mL was intravenously given over 15?min when the hemodynamics were stable. MAP,HR,CVP and urine output were measured and recorded before HSS40 or mannitol infusion(T0), immediately after infusion(T1) and 15，　30，　60 and 120?min after infusion(T25).　Also CSFP was measured and CPP was calculated. ResultsThere were no significant differences in MAP and HR at all time points between the two groups. As compared with their baseline values at T0, CSFP was significantly decreased at T25 in the HSS40 group and at T35 in the mannitol group, and CVP was significantly increased at T24 in the HSS40 group but decreased at T45 in the M group.CSFP was significantly decreased at T3 and CVP increased at T45 in the HSS40 group as compared with the M group. Urine output was significantly higher after infusion(T25) in the mannitol group than in the HSS40 group. ConclusionHSS40 is more effective than 20% mannitol in reducing CSFP and improving hemodynamics during intracranial tumor removal.

    Expression of serum soluble TRAIL in patients with acute cerebral in farction and cerebral hemorrhage
    WANG Xiao1，　BI Jianzhong1，　SUN Zhaohui2，　XIE Zhaohong1，　WANG Yun1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  79-81. 
    Abstract ( 1151 )   PDF (221KB) ( 274 )   Save
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    To explore the changes of sTRAIL in patients with acute phase of cerebral infarction and cerebral hemorrhage. Methods45 cases of acute cerebral infarction and 20 cases of acute cerebral hemorrhage were enrolled in this study, and 20 healthy people served as the control group. The concentration of serum sTRAIL was determined by enzymelinked immuno sorbent assay. ResultsThe concentrations of sTRAIL were（115.94±35.25）and（101.90±16.14）pg/mL in patients with early and late acute phase of cerebral infarction which were higher than those in the control group. There was no significant difference between cerebral hemorrhage and normal control. The sTRAIL concentration was related to the infarction area. ConclusionApoptosis induced by sTRAIL is one of the factors of neuronal damage after cerebral infarction.
    Perioperative application of intraaortic balloon pump in critical patients with coronary disease
    XI Jie, WU Shuming, PANG Xinyan, et al
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  82-84. 
    Abstract ( 1268 )   PDF (270KB) ( 327 )   Save
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    To evaluate the effect of the intraaortic balloon pump (IABP) in critical patients with coronary disease undergoing cardiac operations. MethodsThe clinical data of 38 patients undergoing cardiac operations with application of IABP from August 2001 to February 2008 were retrospectively analyzed. Results25 patients were cured and 13 died, and the mortality was 34.21%. In coronary disease patients with preoperative use of IABP, the mortality was 12.50% (1/8) and in those with intraoperative use, the mortality was 28.57% (6/21), both were much lower than that of the postoperative application group ［66.67%(6/9)］. ConclusionIABP therapy is a safe and effective mode of circulation support. Early application lowers the mortality.
    Time effect on the mechanical properties of orthodontic NT arch wires
    XU Jianguang1, BU Jie2, LI Na1, SONG Nan1, ZHANG Jun1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  85-87. 
    Abstract ( 1296 )   PDF (435KB) ( 246 )   Save
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    To evaluate the time effect on mechanical properties of orthodontic arch wires. Methods30 nonextraction patients treated in the department of orthodontics in Shandong University in the same time period were enrolled in this study. The threepoint bending test was used to measure the strain and stress of arch wires at 37?℃. Oneway ANOVA and q test were used to compare the lag values and platform stresses. ResultsThe platform stress of the control group was 1.54±0.05N and the lag value was 0.47±0.05N. The platform stress of wires with a 4week usage was 1.35±0.03N and the lag value was 0.61±0.03N. The platform stress of wires used for 8 weeks was 1.38±0.07N and the lag value was 0.46±0.07N. The platform stress of wires used for 12 weeks was 1.02±0.04N and the lag value was 0.34±0.04N. By oneway ANOVA and q test, there were significant differences between each group（P＜0.05）. ConclusionWith the increment of use time, the properties of arch wires are attenuated, but their elasticity is still good.
    64slice spiral computed tomography angiography in diagnosis of coronary heart disease
    LIU Pingping1, QIAO Weiwei1, LIU Tongbao2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  88-91. 
    Abstract ( 1714 )   PDF (255KB) ( 250 )   Save
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    To evaluate the clinical values of 64slice computed tomography angiography for coronary heart diseases based on conventional coronary artery angiography serving as the gold standard. MethodsThe results of 64slice CTA were compared with the results of CAG implemented in a recent twoweek period, and the different degrees of the coronary stenosis and pathological changes of different branches of coronary artery shown by 64slice CTA were assessed. ResultsIn 70 patients， 259 coronary arteries were evaluated with 107 lesions found by 64slice CTA. Among the lesions, 101 were diagnosed with CAG; 6 lesions were falsely positive and 8 other lesions were confirmed by CAG, which were not picked up by 64slice CTA. The sensitivity， specificity， positive predictive values and negative predictive values were 92.7%，96.5%，94.4% and 95.4% respectively for detection of the diseases. Conclusion64slice CTA has good sensitivity and specificity in detecting CAD，and it can also give a fairly accurate assessment for stenosis severity in most patients compared with CAG. Therefore，it is a useful noninvasive method for detecting coronary artery diseases.
    natrium for local advanced nonsmall cell lung cancer
    WANG Junchao1,　LIU Huizhong1,　HAO Chengcheng1,　TIAN Guangliang1,　WANG Xianglian2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  91-94. 
    Abstract ( 1354 )   PDF (273KB) ( 331 )   Save
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    癌，非小细胞肺；放射疗法，适形；硝基咪唑类
    The blood glucose, sodium, chloride, potassium and hematocrit of PKRP in the perioperative period
    WANG Jianjun1, ZHAO Zuohui1, SUN Bo1, PAN Yuzhen1, LI Shunlai1,
    WANG Wanli1,　DONG Gang1, MA Tianjia2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  95-97. 
    Abstract ( 1339 )   PDF (252KB) ( 360 )   Save
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    To study the changes of blood glucose, sodium, chloride, potassium and hematocrit (HCT) in patients undergoing plasmakinetic transurethral resection of the prostate（PKRP）in the perioperative period. Methods90 patients undergoing PKRP and 87 patients undergoing transurethral resection of the prostate(TURP) were enrolled in this study, and blood samples were phlebotomized for measurements of blood glucose, sodium, chloride, potassium and hematocrit 1 day before the operation (T1), 20 minutes after the operation (T2), right after the operation (T3), and 4 hours after the operation (T4). ResultsIn the PKRP group, there was a significant decrease of HCT, while there was a significant increase of hemoglucose at T2, T3 and T4; there were no significant changes of the other parameters. While in the TURP group, there was a significant decrease of hemosodium and hemochloride and a significant increase of hemoglucose compared with the PKRP group. ConclusionPKRP is a safe method with little interference of blood glucose, sodium, chloride, potassium and HCT in the perioperative period, and it reduces the incidence of cardiovascular events and prevents the transurethral resection syndrome(TURS).
    Adsorptive voltammetric behavior of prulifloxacin and its application
    ZHU Baolin, GAO Jinhong, LI Jifeng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  98-102. 
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    To explore the Adsorptive Voltammetric Behavior of Prulifloxacin. MethodsIn a supporting electrolyte of 03?mol·L-1KH2PO4NaH2PO4(pH64), a reduction peac of Prulifloxacin (PLF) with a potential of-102?V (vs Ag／AgCL) and adsorptive characteristics were observed by linear sweep voltammetry at the Hg electrode. ResultsThe adsorbed species were most probably the neutral molecules of PLF. The saturated adsorption amount of 401×10-11mol·cm-2 and an area occupied by each molecule of 202?nm2 at Hg electrode for PLF were obtained. On the surface of the hanging mercury drop electrode, the adsorption obeyed a langmuir adsorption isotherm. The adsorption coefficient β, the Gibbs energy of adsorption ΔGθ at 25?℃,　the number of electrons transferred n, the kinetic parameter of the irrepersible system αnα and the rate constant of surface electrode reaction ks　were 108×106,　-2914?KJ·mol-1,　2,101 and 027?s-1, respectively. ConclusionA method for PLF assay was proposed by adsorptive stripping voltammetry. The detection limit of the method is 20×10-8 mol·L-1 under the optimized conditions.
    The resistant starch contents of Luohanshen
    LIU Bin, XU Guifa， HAN Wenting， LIU Ruiping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  103-105. 
    Abstract ( 1426 )   PDF (227KB) ( 825 )   Save
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    To determine the contents of resistant starch in raw and cooked Luohanshen and white potato, taro, sweat potato and yam. MethodsThe contents of resistant starch and total starch were determined before and after cooking in five kinds of tuber crops by a method that mimicked gastrointestinal conditions. ResultsThe contents of resistant starch of 100?g TS in raw and cooked Luohanshen were respectively (77.31±4.22)g and (24.70±2.24)g, both of which were higher than those in other four kinds of tuber crops (P＜0.05). ConclusionThe content of resistant starch is reduced after cooking and is higher in Luohanshen than in other common tuber crops.
    A 2D minimal spanning tree model of the spatial structures of natural focal disease
    TANG Fang1,2, ZHANG Yingqian3, WANG Zhiqiang4, KANG Dianmin4,
    WANG Jiezhen1, XUE Fuzhong1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(01):  106-110. 
    Abstract ( 1230 )   PDF (907KB) ( 429 )   Save
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    To explore the application and geoepidemiological significance of the 2D minimal spanning tree model (MST) in analyzing the spatial structure of natural focal disease. MethodsBased on the foundation of spatial hierarchy clustering and combing graph theory with a path analysis of panbiogeography, we set up the 2D graphic MST model of the framework of geographic information system (GIS). To verify the model, structures of the natural focus of Hemorrhagic fever with renal syndrome (HFRS) of Shandong Province were identified. ResultsThe MST model could not only analyze the comparability of structures but also infer the ecological or epidemiological association and the spatiotemporal trace of diseases. ConclusionThe MST model is a useful method in analyzing spatial structures of natural focal diseases.
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