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    Determination of the Benzo(a)pyrene level in cooking oil by solid
    phase extractionhigh performance liquid chromatography
    YANG Yan, FU Ya-qun, WU Xiao-meng, JIANG Qing-qing, SHAO Li-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (8): 124-127.  
    Abstract1852)      PDF(pc) (1287KB)(121896)       Save

    Objective   To establish a high performance liquid chromatography with fluorescence detector for the determination of the benzo(a)pyrene(B(a)P) level in the cooking oil. Methods   The difference of B(a)P content in edible oil in Jinan market was investigated by the SHIMADZU shim-pack VP-ODS (250mm×4.6mm, 5μm) column. The mobile phase was methanol-water(90∶10, V/V ), and the flow rate was 1. 0mL/min. The samples were treated by the non-bonded silica solid phase extraction(SPE) column, and then detected at the 285nm excitation wavelength and 406nm emission wavelength. Results   The lowest limit of determination was 0.039μg/kg (S/N=3). The recovery rate was 85.8%-90.7%,  and the intra-day and inter-day relative standard deviations were 2.9%-4.1% and 3.7%-5.3%. Conclusion   This method is high sensitive, good selective and quantitative accurate, which is suitable for the B(a)P determination in the cooking oil.

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    Healthcare big data-driven theory and methodology for health management
    XUE Fuzhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (6): 1-29.   DOI: 10.6040/j.issn.1671-7554.0.2017.430
    Abstract1908)      PDF(pc) (9907KB)(4387)       Save
    Todays Internet, cloud computing and networking technology is mature and developed, and the widespread adoption of medical/health information makes medical/health-related data grow at a staggering rate. At the same time, 山 东 大 学 学 报 (医 学 版)55卷6期 -薛付忠.健康医疗大数据驱动的健康管理学理论方法体系 \=-the popularization and application of omics technology(genome, radiomics, etc.)and the rapid development of wearable mobile medical care and mobile health technologies have promoted health care filed quickly into the “big data” era; This suggests that a new era for “health care big data-driven health/disease management” has come, that is, big data-driven health/disease management practices have become a reality. Based on this, we have developed a “health care big data-driven theory and methodology of health/disease management” that covers health/disease detection, risk assessment and intervention. The system includes the health/disease management theoretical framework and conceptual model by the guidance of life course epidemiology and exposome theory under the background of healthcare big data, health/disease detection index screening and evidence acquisition methods, health/disease risk assessment methods and the development of a theoretical approach to health/disease intervention strategies. It is of great theoretical and practical significance to guide the theory and practice development of big data-driven health/disease management, promote the transformation and upgrading of health/disease management and the industrialization of healthcare big data.
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    Rosuvastatin reverses the effect of heavy drinking on angiogenesis after infarction in rats
    QU Fuchao, WANG Aihong, SUN Yongle, WANG Yong, YUAN Haitao, ZHANG Yuying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    DOI: 10.6040/j.issn.1671-7554.0.2013.552
    A general overview of neuromodulation
    Di ZHANG,Meng YU,Xia LIU
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 50-60.   DOI: 10.6040/j.issn.1671-7554.0.2020.0602
    Abstract6488)   HTML391)    PDF(pc) (4269KB)(3822)       Save

    Neuromodulation refers to a series of biomedical engineering techniques which normalize or modulate the function of the nervous system through invasive or non-invasive delivery of physical or chemical stimulus. In the past thirty years, with the in-depth understanding of the nervous system and its mechanisms of action, as well as the rapidly developing modern techniques, neuromodulation has evolved from basic concept to clinical applications. At the same time, new concepts and alternative methods of neuromodulation are constantly being innovated, tested and put into practice. The advancing of neuromodulation techniques will not only provide new tools for basic neuroscience research but also offer novel therapeutic options for neurological and psychiatric disorders. In this overview, we will briefly introduce the current status of different types of neuromodulation techniques, including the clinical applications, underlying mechanisms of their therapeutic effects and advantages/disadvantages, as well as the prospects for the future.

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    Significance of detecting GRACE and TIMI scores for risk stratification and prognosis in patients with acute coronary syndrome
    WANG Wen-wen1, CHEN Yu-guo2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (10): 54-57.  
    Abstract1218)      PDF(pc) (896KB)(3781)       Save

    Objective  To compare GRACE and TIMI scores for risk stratification and prognosis in patients with acute coronary syndrome (ACS), and investigate the predictive significance of these methods. Methods  A  total of 416 patients with ACS were collected during August 2008 to August 2009 in Qilu Hospital of Shandong University for a retrospective case-control study. The GRACE and TIMI scores were recorded and evaluated to analyze the association between incidence of cardiovascular events 30 days and 1 year after the attack and risk scores. Results  The patients with higher GRACE and TIMI scores had higher incidence of cardiovascular events in 30 days and one year. In particular, the patients with GRACE score > 133 and TIMI score ≥ 10 had significantly increased rate of cardiovascular events. There was no significance between the two methods (P>0.05) in predicting high-risk patients. Conclusion  GRACE score and TIMI score are of the same predictive value in risk stratification of ACS patients.

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    Screening results and influencing factors of T-SPOT.TB assay in patients with autoimmune rheumatic diseases
    GAI Linlin, GUAN Lixue, LI Haibo, CHU Jinjin, ZHU Yun, LI Ming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2016, 54 (6): 82-86.   DOI: 10.6040/j.issn.1671-7554.0.2015.713
    Abstract2949)      PDF(pc) (849KB)(3684)       Save
    Objective To investigate the status of latent tuberculosis infection(LTBI)in patients with autoimmune rheumatic diseases and the factors associated with a T-cell enzyme-linked immunospot assay(T-SPOT.TB). Methods During June 2013 and June 2014, a total of 223 patients with various autoimmune rheumatic diseases(patient group)and 53 healthy subjects(control group)were enrolled and screened for LTBI by T-SPOT.TB tests. Data on tuberculosis exposure, use of glucosteroid and disease-modifying anti-rheumatic drugs(DMARDs), BCG vaccination history and abnormal CT scans were collected. Miettinen test was used to estimate odds ratios(OR)and 95% confidence intervals(CI). Results T-SPOT.TB-positive rate was 20.18% in the patient group and 5.66% in the control group, and the patient group had higher risk of LTBI(OR=4.21, 95%CI: 1.21-14.70, P<0.05). There were significant 山 东 大 学 学 报 (医 学 版)54卷6期 -盖林林,等.免疫性疾病患者T-SPOT.TB筛查结果及影响因素 \=-differences in T-SPOT.TB-positive rate and OR among various autoimmune rheumatic diseases. The T-SPOT.TB positive rate was 44.0% in patients with erythema nodosumts(OR=13.11, 95%CI: 3.44-49.98, P<0.01), and 21.16% in patients with rheumatoid arthritis(RA)(OR=4.47, 95%CI: 1.06-18.82, P<0.05), obviously higher than that of controls. The T-SPOT.TB positive rate in patients with other rheumatic autoimmune diseases was 10.0%-33.34%(OR=1.85-8.3, P>0.05). There were no significant differences between the proportions of patients treated with glucocorticoid and DMARDs in T-SPOT.TB-positive and T-SPOT.TB-negative groups(P>0.05). Previous active TB and an abnormal CT examination were significantly associated with T-SPOT.TB-positive rate(P<0.01). Conclusion Patients with autoimmune rheumatic diseases are at increased risk of LTBI(OR=4.21). The glucocorticoid and DMARDs treatment have little effect on T-SPOT.TB results. It is an effective method to screen LTBI with T-SPOT.TB in patients with autoimmune rheumatic diseases.
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    Surgical treatment of Stanford type A aortic dissection
    SUN Yong-hui, ZOU Cheng-wei, LI De-cai, LI Hong-xin, WANG Zheng-jun, ZI Jie, ZHANG Wen-long, ZHANG Hai-zhou, FAN Quan-xin, WANG An-biao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2011, 49 (1): 71-.  
    Abstract1590)      PDF(pc) (414KB)(3550)       Save

    Objective    To review surgical treatment of type A aortic dissection in 65 patients and to investigate the effectiveness of surgical approaches. Methods    Between September, 2003 and April, 2010, 65 patients,  including 45 males and 20 females from 26 to 58 (mean 41.6 ± 9.5) years old, with Stanford type A aortic dissection underwent surgical treatment. Different surgical approaches were adopted according to the rupture site, extent of dissection and aortic valvular insufficiency, including ascending aorta replacement only (10 cases), ascending aorta replacement + arch or semi-arch replacement (13 cases), ascending aorta replacement + arch or semi-arch replacement with stented elephant trunk (27 cases), ascending aorta replacement + four branches covered stent graft implantation (7 cases) and Bentall operation only (8 cases). Concomitant procedures were coronary artery bypass grafting (4 cases), aortic valvuloplasty(12 cases), aortic valve replacement(8 cases ) or Bentall operation (6 cases). Deep hypothermic circulation arrest with selective cerebral perfusion was employed in cases of aortic arch replacement and/or  endovascular stent repair. Results    In the operations, the CPB, clamp and circulation arrest time were 86 -350 (188.3±60.9), 60-248 (101.1±40.0)  and 20-120 (53.9±24.3) minutes, respectively. There were two deaths  early  after operation. Onedied from low cardiac output syndrome  and the other died from renal failure. Postoperative intensive care stay was 1~63 (4.2±8.8) days and the hospital stay was 13~106 (24.8±13.8) days. Complications  consisted of motor aphasia(n=1), cerebral embolism(n=1), pulmonary infection(n=3), renal failure(n=1), hemorrhage(n=5), mental disorder(n=4), tracheotomy (n=1), and voice hoarseness(n=1). Conclusion     Aortic dissection is the most common catastrophic event that involves the aorta, however, the prospects of early surgical treatment are satisfying.  The key points are quick, correct diagnosis and proper surgical approaches.

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    Characteristics of homosexual behaviors and relationship between high-risk sexual behaviors and seeking sex partners through the internet in men who have sex with men among university students
    ZHENG Jietao, ZHANG Xiaofei, L(¨overU)Cuixia, FU Jihua, MA Wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (7): 115-123.   DOI: 10.6040/j.issn.1671-7554.0.2017.016
    Abstract2299)      PDF(pc) (957KB)(3170)       Save
    Objective To understand the characteristics of sexual behaviors and relationship between seeking sex partners through the internet and high-risk sexual behaviors in men who have sex with men(MSM)among university students in a city. Methods A survey was conducted in MSM among college students who were recruited through convenience sampling in a city of Shandong Province, China. The relationship between seeking sex partners through the internet and high-risk sexual behaviors was analyzed with multivariate Logistic regression analysis. Results A total of 300 valid questionnaires were collected. Among the participants, the average age of the first male-to-male sex was(17.6±1.8)years old; 7.7% had two or more male sex partners last week; 35.7% did not use condoms and 5% was involved in group sex in last male-to-male sex; 6.7% had commercial sex for money or drugs. About 86% of the participants seek sexual partners via the internet. Multivariate Logistic regression analysis showed negative relations between seeking sexual partners through the internet and having two or more male sex partners (OR=0.28, 95%CI=0.11-0.73) 山 东 大 学 学 报 (医 学 版)55卷7期 -郑杰滔,等.高校学生男男性行为特征及其高危性行为与互联网交友的关联 \=-and commercial sex(OR=0.22, 95%CI=0.07-0.66). Conclusion High-risk sexual behaviors are common among university MSM students. University MSM students who seek sexual partners through the internet have less high-risk sexual behaviors than those who seek sexual partners via non-internet ways.
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    Effect of double-lumen-tube or bronchial blocker used for one-lung ventilation on PetCO2 and PaCO2
    YANG Xiaomei, FANG Ningning, YI Wenbo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2015, 53 (11): 77-79.   DOI: 10.6040/j.issn.1671-7554.0.2015.262
    Abstract1488)      PDF(pc) (1526KB)(3140)       Save
    Objective To assess the effect of double-lumen-tube (DLT) and bronchial blocker for one-lung ventilation on partial pressure of end-tidal carbon dioxide (PetCO2) and arterial partial pressure of carbon dioxide (PaCO2). Methods Thirty patients were randomly assigned to DLT group (n=15) and BB group (n=15). After anesthesia induction, DLT or BB was intubated into the trachea. After one-lung ventilation, the values of PetCO2 and PaCO2 were recorded and analyzed. Results Compared with BB group, the PetCO2 value was higher, the PaCO2 value was lower, and the correlation between PetCO2 and PaCO2 was better in DLT group. Conclusion Compared with BB, DLT was better at exhalation of CO2.
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    Heterogeneity of human peripheral and decidual monocytes of women in early pregnancy
    ZHANG Yun 1,2, SUN Jin-tang 2, HUANG Xian-liang 3, YANG Mei-xiang 2,SHAO Qian-qian 2, DENG Bi-ping 2, XU Xiao-fei 3,MAO Hai-ting 2, DONG Ba
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (3): 98-101.  
    Abstract1693)      PDF(pc) (444KB)(2951)       Save

    Objective    To analyze the distribution of human CD14+CD16- and CD14+CD16+ monocyte subsets in peripheral blood and decidua of women in early pregnancy. Methods    Peripheral blood samples were collected from early pregnant and nonpregnant females. Mononuclear cells were isolated from the fresh decidua of early pregnant females by mechanical trituration and Ficoll gradient centrifugation. The fluorescent intensity of the cells was detected by FCM. Results    The percentage of the peripheral CD14+CD16- monocyte subtype in early pregnant females was lower than that in  non-pregnant controls(P<0.01). CD14+CD16+ cells (69.13%±7.73%)  and were the major monocyte subset in the decidua of early pregnant females. Conclusion    CD14+CD16+ monocytes become the predominant cell subpopulation of women in early pregnancy. It has been demonstrated that CD14+CD16+ monocyte-derived dendritic cells are efficient producers of anti-inflammatory interleukin-10, so CD14+CD16+ monocytes  might play an important role in forming and keeping the dominance of TH2 cytokine during early pregnancy.

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    Contents and ratios of type I and type III collagens in normal skin and hypertrophic scar in people of different ages
    WANG Cheng, RONG Yanhua, SHEN Yuming, ZHANG Guoan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2016, 54 (11): 64-67.   DOI: 10.6040/j.issn.1671-7554.0.2015.1043
    Abstract4659)      PDF(pc) (1915KB)(2637)       Save
    Objective To quantify the contents of type I and III collagens and their ratios in normal skin and hypertrophic scar in people of different ages, and to explore the rule and cause of changes. Methods Normal human skin specimens and hypertrophic scar specimens were obtained from spontaneously aborted fetus and people of different ages, including the children and adolescent group(≤18 years), adult group(19-50 years), elderly group(>50 years). The total collagen contents were determined by hydroxyproline method. The ratios of types I and III collagens were examined by immunohistochemistry, and their contents were calculated. Results The difference of the contents of types I and III collagens and their ratios in normal skin were statistically significant among different age groups(P=0.01). Their ratios increased with the increase of age, and the differences were statistically significant among different age groups(P=0.02). The difference of contents of types I and III collagens and their ratios in hypertrophic scars were statistically significant among all groups, also between any two groups(P=0.03). In every age group, the difference of the contents of types I and III collagens and their ratios between normal skin and hypertrophic scar was statistically significant (P=0.02). Conclusion With age increasing, the changes of the ratios of types I and III collagens in the normal skin changes, which results in changes of the tension and elasticity of skin and the healing process after injury. The contents of types I and III collagens and their ratios significantly changes in hypertrophic scars, resulting in different structure of scar from normal skin.
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    Locmotion control of quadruped robot based on deep reinforcement learning: review and prospect
    Wei ZHANG,Wenhao TAN,Yibin LI
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 61-66.   DOI: 10.6040/j.issn.1671-7554.0.2020.0607
    Abstract4232)   HTML267)    PDF(pc) (1128KB)(2549)       Save

    Brain-inspired deep reinforcement learning has recently led to a wide range of successes in different domains such as artificial intelligence and robotics. The method combining both advantages of deep learning and reinforcement learning gets strong capability of perception and decision-making. In this paper, we first provide a brief overview of two kinds of widely used deep reinforcement learning method and their fundamentals, then introduce the current status of deep reinforcement learning applying on quadruped robots. Finally, by summarizing the existing methods and the characteristics of locomotion for quadruped robots, we present future potential of deep reinforcement learning on quadruped robots.

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    Evolution of diagnostic criteria for Alzheimers disease: from clinical diagnosis to pathophysiological diagnosis
    TANG Yi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (10): 14-20.   DOI: 10.6040/j.issn.1671-7554.0.2017.320
    Abstract2253)      PDF(pc) (1150KB)(2487)       Save
    Alzheimers disease(AD)is the leading cause of dementia and the most prevalent neurodegenerative disease in the elderly. The first diagnostic criteria was developed by the National Institute of Neurological and Communicative Disorders and Stroke and the Alzheimers Disease and Related Disorders Association Work Group in 1984. With the further understanding of pathophysiological mechanism underlying AD, two new sets of research criteria for Alzheimers 山 东 大 学 学 报 (医 学 版)55卷10期 -唐毅.阿尔茨海默病诊断标准:从临床诊断到病理生理诊断 \=-disease were proposed: those published by an International Working Group in 2007, which was updated in 2014, and the recommendations published by the National Institute on Aging and the Alzheimers Association(NIA-AA)in 2011. A comparison of the criteria is needed for clinical practice and research purpose.
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    Effect of traditional Chinese medicine for kidney-reinforcingof morphological alterations of the adrenal gland in  mice with Kidney-Yang deficiency
    XU Cui-ping1,LI Zhen2,SONG Jie3,ZHANG Dan2,ZHU Qing-jun2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2011, 49 (2): 67-70.  
    Abstract1612)      PDF(pc) (1492KB)(2434)       Save

    Objective    To explore the effect of traditional Chinese medicine for kidneyreinforcing of  morphological alterations of the adrenal gland in mice with Kidney-Yang deficiency. Methods    The model of Kidney-Yang deficiency was established by overstrain and excessive sexual intercourse. The mice were randomly divided into three groups, including the normal control group, the Kidney-Yang deficiency group, and the Jinkuishenqi bolus group. After four-week treatment of traditional Chinese medicine for kidney-reinforcing, morphological changes of the adrenal gland were observed in all groups. Result    Compared with the normal control group, the weight and coefficient of the adrenal gland in the mice with Kidney-Yang deficiency were both obviously decreased(P﹤0.01; P﹤0.05). Under a light microscope, it was shown that in the group of Kidney-Yang deficiency,   the adrenal cortex became thin, and the zona glomerulosa and fasciculate were narrow. The structure of cells was  irregularly arranged, while the cellular volume was reduced with shallow staining. In addition, vacuole-shaped cytoplasm and widened intercellular space were identified. Under an electronic microscope,  the shapes of nuclei were irregular in the glomerulosa zone of the adrenal cortex, while the nuclear membrane dissolved, the outer nuclear membrane protruded into the cytoplasm, and the chromatin were clumped. Compared with the normal control group, the number of mitochondria in the Kidney-Yang deficiency group was remarkably decreased, and parts of them were swollen and degenerated. The mitochondrial cristae were concentric or fingerprint-shaped and rough endoplasmic reticulum in the Kidney-Yang deficiency group were fewer than that in the normal control group. Smooth endoplasmic reticulum was obviously enlarged and vesicle-shaped. In contrast to the untreated group, the morphology of the adrenal cortex in the Jinkuishenqi bolus group was improved to a certain degree, which was similar to the normal control group. Conclusion     Morphology and ultra-structure of the adrenal gland in  mice with Kidney-Yang deficiency demonstrate degenerative changes. However, such damages can be repaired if treated with Jinkuishenqi bolus.

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    Estimation of reference range for thyroid functional parameter in pregnant women of Jinan City, China
    SHEN Yan, ZUO Changting
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2016, 54 (5): 34-38.   DOI: 10.6040/j.issn.1671-7554.0.2015.053
    Abstract1590)      PDF(pc) (811KB)(2349)       Save
    Objective To establish the refernce ranges for specific serum FT3, FT4 and TSH in different stages of the pregnant women of Jinan City, China. Methods A total of 370 pregnancy women were chosen as the study group, and 60 non-pregnancy women were chosen as the control group. According to the guidelines of United States National Institute of Clinical Biochemical(NACB), the serum levels of TSH, FT4, FT3, thyroglobulin antibody(TgAb), thyroid peroxidase antibody(TPOAb), thyrotrophin receptor antibody(TRAb)were detected by electrochemiluminescence method. Results The reference ranges of the serum levels of FT3, FT4 and TSH during the early, medium, and late periods were 3.33-5.78, 3.07-5.52, 2.91-5.46 pmol/L; 12.60-18.94, 8.74-17.23, 8.64-17.21 pmol/L; and 0.27- 4.27, 0.53- 4.75, 0.76- 4.59 mIU/L. Conclusion The reference ranges of the serum levels of FT3, FT4 and TSH at the different gestation stages in Jinan City have a large difference between the pregnant women of the other areas, and also have a large difference of the reference ranges of the pregnant women recommended by ATA.
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    Current status and prospect of viral vaccines research and development
    Kai WANG
    Journal of Shandong University (Health Sciences)    2021, 59 (5): 8-14.   DOI: 10.6040/j.issn.1671-7554.0.2021.0273
    Abstract4654)   HTML594)    PDF(pc) (1285KB)(2348)       Save

    Virus infection can cause several diseases in human. In the past decades, outbreaks and epidemics of respiratory infectious diseases caused by coronary viruses, especially coronavirus disease 2019 (COVID-19), have become serious health hazards and posed huge challenges to public health. The development and application of viral vaccines have been an important means to counteract the spread of viruses. Historically, poliovirus and hepatitis B virus vaccines have successfully suppressed the transmission of diseases and provided a model for the development of vaccines. Recently, the emergence of novel technologies and platforms such as recombinant viral vector and mRNA vaccine has provided new approaches to the development of vaccines against emerging infectious diseases including COVID-19. This paper reviews the current situation and progress of platforms, summarizes problems to be solved, and forecasts the directions of viral vaccines development.

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    Evaluation of efficacy and safety of manidipine hydrochloride among patients with mild or moderate essential hypertension: a multicenter, randomized, doubleblind study
    MA Xiao1, WANG Yue-ming2, LI Hong-shi3, KAN Xiao-hong4,ZHAO Jing4, YUE Xin4, ZHANG Yun4, ZHONG Ming4, LI Li4
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (8): 58-61.  
    Abstract1306)      PDF(pc) (944KB)(2319)       Save

    Objective   To evaluate the efficacy and safety of manidipine hydrochloride among patients with essential mild or moderate hypertension. Methods   Two hundred and forty subjects with mild or moderate hypertension were enrolled in the study and assigned to two groups (the study group and the control group) equally by a randomized, double-blind,multicenter and positive controlled study.After a one to two-week placebo run-in period, patients with essential mild or moderate hypertension were distributed randomly into two groups to receive study drug (manidipine hydrochloride 10~20mg per day) or positive controlled drug ( amlodipine besylate 5~10mg per day) respectively for 8 weeks. BP change, tolerance and adverse events between the two groups were evaluated. Results   One hundred and three patients completed trial in the study group and 103 patients in the control group.Subjects who completed the study were with good compliance,except those dropped off or were excluded.There was no significant difference for baseline characteristics between the two groups (P>0.05) including age,sex,body mass index,baseline systolic and diastolic BP,and heart rate.The reduction of diastolic and systolic BP, heart rate and seat diastolic blood pressure normalized rate between the two groups had no significant difference at the end of treatment (P>0.05). The incidence of adverse events also had no significant difference between the two groups(P>0.05). Conclusion   The antihypertensive efficacy of manidipine hydrochloride is equal to amlodipine besylate among patients with mild or moderate hypertension. Manidipine hydrochloride is safe and can be well tolerated.

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    A new field of therapeutic interventional pulmonology——clinical application of tracheal T tube
    HUANG Haidong, ZHENG Guiliang, WANG Qin, YANG Yuguang, LI Qiang, BAI Chong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (4): 23-29.   DOI: 10.6040/j.issn.1671-7554.0.2017.165
    Abstract2400)      PDF(pc) (1591KB)(2305)       Save
    With the rapid development of rigid bronchoscopy and interventional pulmonology in China, Chinese pulmonologists who are engaged in the practice of interventional pulmonology now have the conditions of tracheal T tube placement by rigid bronchoscope technique for the treatment of proximal tracheal stenosis. The tracheal T tube placement by rigid bronchoscope technique has a certain minimally invasive advantage compared to that by traditional surgery technique. However, all the clinical problems usually can not be solved by a single technique due to the complex situations of laryngotracheal stenosis. Better clinical results and low complications for laryngotracheal stenosis are related to the understanding of the difference between two techniques, developing multidisciplinary treatment approaches and strictly controlling of the indications of tracheal T tube placement.
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    Package and expression of lentivirus encoding human GCSF genes
    LIN A-li,SUN Qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1716)      PDF(pc) (321KB)(2244)       Save
    Objective: To construct a lentiviral vector encoding human granulocytemacrophage colony stimulating factor(hGM-CSF) genes and to transfect CT26 cells in order to establish the CT26 strains which has been continuously overexpressing the hGM-CSF gene. Methods: DNA fragments of human GM-CSF were amplified by PCR and were inserted into the vector plasmid L166. The three plasmids expressing lentivirus were packaged into virus packaging cell line 293T using the CaCl2 method. After 72 hours of transfection, the virusproducing cell supernatant was produced. CT26 cells were transfected by lentivirus and the titration was determined. Results: The sequence of the PCR product in the recombinant plasmid was produced. The three plasmids were effectively transferred into 293T. When the CT26 cells were infected as target cells by the lentivirus supernatant, the titration of lentivirus was 4.69×105IU/ml. hGM-CSF was determined in the supernatant of CT26 cells transfected by lentivirus, which was kept for more than two months. The transfected CT26 cells were screened by puromycin at a terminal concentration of 15μg/ml. Conclusion: The lentivirus encoding hGMCSF genes were successfully constructed.
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    Mucus fills the intestinal epithelial gaps and maintains the barrier function
    ZHOU Xiaoyan, LI Ming, GUO Jing, ZUO Xiuli, LI Yanqing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2015, 53 (12): 62-66,80.   DOI: 10.6040/j.issn.1671-7554.0.2015.148
    Abstract1273)      PDF(pc) (3773KB)(2152)       Save
    Objective To investigate whether mucus fills the intestinal epithelial gaps(IEG), and to explore the role of mucus in maintaining local barrier function. Methods The terminal ileum tissues of ulcerative colitis (UC) patients and controls were obtained. Mucins were stained with Alcian Blue-Periodic Acid Schiff (AB-PAS) and Muc2. In addition, we also analyzed the confocal laser endomicroscopy (CLE) imagines of the terminal ileum from 39 UC patients and 34 controls since January 2013 to December 2014. Revised Watson grading system was used to evaluate the local barrier function. Results In UC patients, the proportion[M(P25,P75),%] of IEG without mucus, intestinal epithelial gaps, goblet cells were 27.52(25.00,29.68), 5.33(3.48,6.62) and 17.38(16.00,19.87), respectively. In control group, these three parameters were 12.27(9.78,16.67), 0.27(0.00,0.69) and 23.44(20.24,26.18), respectively. The differences were statistically significant in the two groups (P<0.01). The proportion of intestinal epithelial gaps and goblet cells showed a significantly negative correlation(r=-0.74, P<0.01). Conclusion The local barrier function is lost in UC patients; mucus filled with IEG play a key role to maintain the local intestinal barrier function.
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    Prevention and control of emerging viral infectious diseases and biosafety
    Hongling WEN,Xiaoying XU
    Journal of Shandong University (Health Sciences)    2021, 59 (5): 40-45.   DOI: 10.6040/j.issn.1671-7554.0.2021.0308
    Abstract3147)   HTML247)    PDF(pc) (1430KB)(2149)       Save

    In recent years, the frequent occurrences of emerging infectious diseases, especially the interspecies transmission of animal-borne viruses, have brought immense challenges to the prevention and control of infectious diseases and biosafety, such as laboratory biosafety, frontier port biosafety and dual-use biotechnology. Currently the international biosafety situation is serious, characterized with internationalization, extreme harm and complicated development. Biosafety has become a major issue endangering national security, which has aroused great concern about virology and biological threats. In this paper, the occurrence, prevention and control of emerging viral infectious diseases and the status quo of biosafety in basic research were reviewed, various problems were summarized, and the future development of biosafety was prospected.

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    Hemostatic effect of hemocoagulase used in bladder irrigation and
    intravesical instillation after transurethral resection of the prostate
    DING Sen-tai1, ZHAO Peng1, L Jia-ju1, XING Nai-dong1, NIU Zhi-hong1, SUN Hui2, WU Hai-hu1, SUN Ding-qi1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (10): 93-96.  
    Abstract2453)      PDF(pc) (959KB)(2127)       Save

    Objective   To evaluate the hemostatic effect of hemocoagulase used in bladder irrigation and intravesical instillation after transurethral resection of the prostate (TURP). Methods   From March 2010 to March 2012, 69 selected patients who underwent TURP were randomly divided into three groups, and all patients were administrated with postoperative continuous bladder irrigation using  NS conventionally. 20 cases in the intravesical instillation group were given postoperative intravesical instillation using 6U hemocoagulase in 60mL NS for 20min, bid for 3d. 23 cases in the bladder irrigation group were given bladder irrigation using 10U hemocoagulase in 100mLNS, bid for 3d. The rest of 26 patients were assigned to the control group with continuous bladder irrigation using normal saline (NS). The bleeding volumes in the 3 days after surgery of the 3 groups were recorded and analyzed by using SPSS16.0 software and the coagulation function and adverse effects were observed. Results   There was no significant difference of patients′ age, prostate volume or operation time among the three groups, additionally, no obvious coagulant function change and complications occurred in the patients of the three groups. Compared with that of the control group, the total bleeding volumes of the postoperative 3 days in the experimental groups of hemocoagulase were significantly reduced (P<0.05). However, for 3 days′ total or each day′s blood bleeding volume, no significant difference was observed between the bladder irrigation group and the intravesical instillation group. Conclusion   Local application of hemocoagulase was effective and safe in reducing postoperative bleeding after TURP.

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    β-catenin expressions in different subtypes of breast cancer and its clinical significance
    LI Shan-shan, SUN Ying, DING Huan, LI Li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (3): 107-110.  
    Abstract1271)      PDF(pc) (2010KB)(2073)       Save

    Objective   To investigate the expressions of β-catenin in 1different subtypes of breast cancer and its clinical significance. Methods   β-catenin expression was detected in 38 cases of triple negative breast cancer(TNBC)、 131 cases of non-TNBC and 10 cases of normal breast tissues by using tissue microarrays and immunohistochemistry. Results   β-catenin protein was only observed on the cytomembrane of normal breast tissues;However, β-catenin protein was not only observed on the cytomembrane of breast cancer tissues, but also in the cytoplasm and nucleus. Abnomal β-catenin expression was higher in TNBC cases(78.9%) than that in non-TNBC cases(60.3%)(P<0.05). An association was identified between abnormal β-catenin expression and lymph node metastasis, histological grade, TNM stage, Ki67-positive and prognosis(P<0.05). Conclusion   β-catenin may play an important role in breast cancer tumorigenesis and progress, and it could be used as a new indicator for the biological behavior、prognosis and targeted therapy in breast cancer hopefully.

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    Detection and significance of CD4+CD25+ Foxp3+ regulatory T cells in peripheral blood of patients with vitiligo
    LI Qiang1,2, CAI Da-xing1, CHEN Guang3, LIU Ji-feng4, LI Chun-yang1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (1): 133-.  
    Abstract1306)      PDF(pc) (3172KB)(2058)       Save

    Objectives   To detect CD4+CD25+Foxp3+ regulatory T cells in peripheral blood of patients with vitiligo, and to evaluate immunomodulator′s therapeutic effects. Methods   36 patients with vitiligo were selected, including 24 with active vitiligo and 12 with stationary vitiligo. The level of CD4+CD25+Foxp3+ regulatory T cells in peripheral blood was detected by flow cytometry before and after treatment with immunomodulator, 20 healthy subjects as controls. Results   CD4+CD25+Foxp3+ regulatory T cells in active vitiligo were significantly lower than in normal controls(P<0.05).  There was no statistical difference between stationary vitiligo and normal controls(P>0.05). CD4+CD25+Foxp3+ regulatory T cells in active vitiligo increased after comprehensive treatment with immunomodulator(P<0.05); while there was no statistical difference in stationary vitiligo after treatment with immunomodulator(P>0.05). Conclusion   CD4+CD25+Foxp3+ regulatory T cells could control the autoimmune reaction by inhibiting autoreactive T cells. The abnormal number of CD4+CD25+ Foxp3+ regulatory T cells in peripheral blood may cause imbalance of immunity. Polysaccharide nucleic acid fraction of bacillus calmette guerin(BCGPSN) could modulate the cell immune function by regulating the level of CD4+CD25+ Foxp3+ regulatory T cells and plays therapeutic effects.

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    Advances in the application of artificial intelligence in coronary computed tomography angiography
    Guyue ZHAO,Jin SHANG,Yang HOU
    Journal of Shandong University (Health Sciences)    2023, 61 (12): 30-35.   DOI: 10.6040/j.issn.1671-7554.0.2023.0795
    Abstract1280)   HTML57)    PDF(pc) (1479KB)(2038)       Save

    With the increasingly widespread application of artificial intelligence in the field of medical imaging, its application in coronary artery CT angiography has shown great potential, which helps to improve image quality, optimize post-processing processes, assist disease detection, evaluate functional status, analyse prognosis, and other aspects. Meanwhile, there arise some problems, and the full inspection process should be further optimized to enhance its practicality and efficiency. This article reviews the research progress, existing problems, and future development of artificial intelligence in coronary artery CT angiography.

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    Review of mental health assessment of the elderly based on different theoretical models
    PAN Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (9): 1-5.   DOI: 10.6040/j.issn.1671-7554.0.2017.472
    Abstract1902)      PDF(pc) (1058KB)(2033)       Save
    China entered the aging society in the year 2012, in which people aged ≥60 years accounted for 10% of the total population. In order to meet the needs of life care, health care and rehabilitation care, as well as to provide professional service, it is necessary to evaluate the mental health status of the elderly. At present, there exist different mental health standards and assessment methods based on multiply theoretical models. This article reviews the standards of psychological health and evaluation methods based on pathological psychology, mental health, positive psychology and positive aging.
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    Preparation of melatonin liposomes and their physico-chemical properties
    SUN Qingxue, HUANG Guihua, SHAO Wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (1): 159-163.  
    Abstract1663)      PDF(pc) (483KB)(2010)       Save

    Objective  To study the preparation of melatonin (MLT) liposomes and their physico-chemical property. Methods  Liposomes were prepared by a film dispersion method. Based on a single test, the optimum formulations and the preparation technology were optimized by a uniform experiment. Morphology of the liposomes was detected by transmission electron microscopy. The Zeta potential and particle size distribution were evaluated by laser scattering. High speed centrifugation was applied to separate the free drug and liposomes. The entrapment efficiency and drug loading were determined by UV. The release character of melatoninliposomes was studied by the dialysis method. Results  The optimal formula was obtained by a uniform design: the phosphatides to cholesterol ratio was 25∶1, and the drug to lipid ratio was 1∶10, 40℃. The liposomes were a multi-layer spherical orellipsoidal shape with a uniform size. The mean diameter of melatonin liposomes  was(5.542±0.04)μm. The Zeta potential was -31.68?mV. The drug loading and entrapment efficiency of melatonin liposomes were (77.23±2.51)% and (3.60±0.29)%, respectively. In vitro release could be sustained for 72h and conformed to bio-exponential kinetics (rα=0.988?6 and rβ=0.9874). Conclusion  Melatonin liposomes made by using the film dispersion method have a high encapsulation efficiency and have a good delayed-release property in vitro.

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    Reliability and validity of Chinese Version of the Cultural Value Orientation Scale and correlation between cultural value orientation and psychological health
    LI Huafang, ZHANG Hongjing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (9): 122-127.   DOI: 10.6040/j.issn.1671-7554.0.2016.1292
    Abstract2249)      PDF(pc) (930KB)(1985)       Save
    Objective To validate the reliability and validity of the Chinese Version of Cultural Value Orientation(CVO)among Chinese youth and to explore the correlation between cultural value orientation and psychological health of the youth. Methods A total of 938 Chinese youth were surveyed with CVO. The scales reliability and validity were analyzed with Cronbach α, exploratory factor analysis(EFA), and confirmatory factor analysis(CFA).The correlation between cultural value orientation and psychological health was analyzed with the general health questionnaire(GHQ-28). Results The Cranach α of familism, individualism and collectivism was 0.88, 0.73 and 0.84, respectively. The CFA models RMSEA indices were 0.04, 0.03 and 0.05(RMSEA≤0.05). Psychological health(GHQ)was significantly correlated with familism and collectivism(r=-0.24, r=-0.14, P<0.01), but not with individualism(r=-0.06, P>0.05). Conclusion The Chinese Version of CVO has good reliability and validity. Familism and collectivism have significant effects on psychological health.
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    Retrospective study of 868 port-wine stains patients treated with flashlamp-pumped pulsed dye laser(595nm)
    ZHUO Shao-yang1,2, WANG Rui1, LI Kai1, LIU Shao-hua1,3, ZHANG Xian-jun4
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (12): 100-102.  
    Abstract1473)      PDF(pc) (896KB)(1980)       Save

    Objective   To evaluate the efficacy of 595 nm flashlamp-pumped pulsed dye laser in treatment of port-wine stains(PWS). Methods   A retrospective study was performed in 868 patients with PWS treated with 595nm flashlamp-pumped pulsed dye laser. The efficacy and side-effects were evaluated. Results   The total effective rate was 71.54%. The efficacy was correlated with patients′ age, sites of lesions, area of lesions and the times of treatment (P<0.05). Blister, hyperpigmentation, and scar were found in 0.9%, 0.4% and 0.7% of these patients, respectively. Conclusion    595nm flashlamp-pumped pulsed dye laser is effective and safe for the treatment of PWS.

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    The latest advances in human brain projects
    Xingang LI,Xin ZHANG,Anjing CHEN
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 5-9, 21.   DOI: 10.6040/j.issn.1671-7554.0.2020.0606
    Abstract2280)   HTML143)    PDF(pc) (1284KB)(1951)       Save

    With the rapid development of modern technology, we have extended in-depth knowledge of the surrounding world from both macro and micro perspectives. However, we know very little about the fine structure and operation mechanism of human brain. Therefore, many countries and regions have proposed and launched human brain projects to deepen our understanding of human brain and to develop novel treatment for the nervous and psychiatric system related disorders. This paper reviews the human brain projects implemented by the European Union, the United States, Japan and China in recent years, and introduce the key research directions and breakthroughs. We will also proudly introduce the "Qilu Brain Project" carried out by our institute and briefly present our project progress. The implementation of human brain projects is conducive to promoting the progress of artificial intelligence and other related disciplines as well as economic and social development and needs more in-depth international cooperation.

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    Significance of ETV6 rearrangement in acute myelogenous leukemia-M4Eo
    GAO Na,LI Zhi-hong,DING Bu-tong,CHEN Yun,WANG Yun-shan,GUO Nong-jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1753)      PDF(pc) (314KB)(1950)       Save
    To explore the clinical significance of ETV6 gene rearrangement involved in acute myelogenous leukemia M4Eo. Methods3 cases of AML -M4Eo were prepared the chromosome after the bone marrow cells culture. R-banding technique was used to analyze the karotypes. 12p13 translocation was determined by Split-signal FISH in the interphase and metaphase cells. ETV6/ARG fusion genes were analyzed by RT-PCR. ResultsClone chromosomal abnormalities with inv(16) was found by Karotype analysis in 3 cases, abnormal fluorescence signal with 12p13 translocation was found in 1 case by split-signal FISH, and ETV6/ARG fusion gene was found by RT-PCR. This was the second report that ETV6/ARG expressed in AML-M4Eo. ConclusionThis patient is not sensitive to chemical therapy, even has worse prognosis than patient without this fusion gene. Splitsignal FISH is a reliable measure to detect ETV6 gene rearrangement.
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    Expression of mucins (MUC1, MUC2, MUC5AC and MUC6) and
    their significance in invasive ductal breast carcinoma
    ZHUANG Ting1, XUE Mei2, FANG Ai-ju3, GUAN Bing-xin1, LIANG Yan1, WANG Zhi-hui2, WANG Yan1, MENG Bin1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (5): 107-111.  
    Abstract3441)      PDF(pc) (2964KB)(1933)       Save

     Objective   To evaluate expression of several mucins in invasive breast ductal carcinoma and the correlation with clinicopathological features. Methods   Tissue microarray (TMA) and immunohistochemical(IHC) technologies were used to evaluate expression of several mucins (MUC1, MUC2, MUC5AC and MUC6) in 207 cases of invasive breast ductal carcinoma and to analyze their correlation with clinicopathological features. Results   MUC1 expression was detected in 89.4% of cases and its expression was associated with lower histological grade, estrogen receptor (ER) positive and absence of lymph node (LN) metastasis. The subcellular localization of MUC1, but not expression level, had a prognostic value in postoperative recurrence and metastasis. MUC2 expression was noticed only in 4.3% of all cases. MUC5AC and MUC6 were expressed in 42.0% and 22.2% of cases respectively. There were associations between MUC5AC and lower histological grade as well as ER-positive, and MUC6 was positively correlated with HER-2. But there was no association between mucins and other clinicopathological variables. Conclusion   WhileMUC1 is expressed in the majority of the cases, others (MUC2, MUC5AC, MUC6) are not. Ectopic expression of MUC1 might be a potential prognostic indicator, which needs further study for confirmation.

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    Polymorphisms of Fg Bβ448 and PAI-1 4G/5G in patients with coronary heart diseases
    GAO Hui,GE Ru-cun,ZHOU Hai-ping,ZHANG Min
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1837)      PDF(pc) (226KB)(1923)       Save
    Objective: To study the correlation between the Fg Bβ448 polymorphisms and their mRNA expression and fibrinogen(Fg) level, and the correlation between plasminogen activator inhibitor-1(PAI-1) 4G/5G polymorphisms and their mRNA expression and PAI-1 activity(PAI-1:A), PAI-1 antigen(PAI-1:Ag) level in patients with coronary heart disease(CHD). Methods:  The subjects were divided into two groups: the control group and the CHD group. Contents of Fg(Clauss), PAI-1:A(Chromogenic assay) and PAI-1:Ag(ELISA) in the plasma were determined. PCR-RFLP was used to determine the Fg Bβ448 and the PAI-1 4G/5G polymorphisms, and RT-PCR was used to determine the expression of their mRNA. Results: This polymorphism is related to elevated Fg mRNA expression. The mRNA expression degree was as following: L/L genotype, the highest; A/L, the middle; and A/A, the lowest. There was a positive correlation between mRNA expression and plasma Fg level. The frequency of A/A genotype of Fg Bβ448 had no statistical difference between the CHD group and the control group (P>0.05). Different genotypes offered different effects on PAI-1 mRNA expression. Expression of the 4G/4G genotype was in the highest, of the 4G/5G genotype was in the middle, and the 5G/5G genotype was the lowest. The frequency of the 4G/5G genotype of PAI-1 was significantly higher in the CHD group than that in the control group (P<0.05), contributing to over-expression of PAI-1 mRNA. There was a positive correlation between mRNA expression and plasma PAI1:A and PAI-1:Ag level. Conclusions: Patients with CHD are hyper coagulation and fibrinolysis deficient, but elevated Fg may be related to the gene type and the environment. However, the environment may have an effect on the PAI-1 expression, but PAI-1 polymorphism is more important than the environment.
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    Atypical presentations of benign childhood epilepsy with centrotemporal spikes
    LI Jing, LI Bao-min, LEI Ge-fei, JIA Gui-juan, TONG Li-li, WANG Ji-wen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (7): 42-44.  
    Abstract1502)      PDF(pc) (974KB)(1922)       Save

    Objective   To explore the atypical clinical presentation, electroencephalography characteristics, treatment and prognosis of benign childhood epilepsy with centrotemporal spikes (BECT). Methods   We collected the clinical data of 122 BECT children who were treated in Qilu Hospital of Shandong University from September 2006 to July 2012. Ambulatory or video electroencephalography monitoring was performed in 11  BECT children who had atypical symptoms screened from the above 122 children. The seizure semeiology, neuropsychological impairment and response to antiepileptic drugs were analyzed and followed up in the 11 children. Results   All of the 11 cases showed seizure changes or electroencephalography deterioration. Sodium valproate was only effective in 2 cases. Combination of sodium valproate and clonazepam was effective in 4 cases. Topiramate were effective in 3 cases. Electrical status epilepticus still existed during sleep after various antiepileptic drug therapy in 2 cases. Conclusion   Atypical presentations and  different forms of seizure semeiology exist in BECT. The clinical presentations are associated with electroencephalography deterioration and cognitive deficits, and the seizures are relatively difficult to control.

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    Associations of maternal methionine synthase A2756G and methionine synthase reductase A66G polymorphisms with neural tube defects of the offspring: A meta-analysis
    ZHANG Bing-zhen, DAI Bing-qin, ZHANG Xing-liang, WANG Zhi-ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (7): 125-132.  
    Abstract1461)      PDF(pc) (3609KB)(1910)       Save

    Objective   To evaluate associations of maternal methionine synthase (MTR) A2756G and methionine synthase reductase(MTRR) A66G polymorphisms with the offspring′s risk for neural tube defects (NTDs). Methods   The retrieval strategy and criteria for inclusion and exclusion were made. Literature was identified by searches for case-control studies on maternal MTR A2756G or MTRR G66A polymorphism and risk of NTDs in the offspring published in English or Chinese from following databases: China Biology Medical Literature Database(CBM), Database of Chinese Scientific and Technical Periodicals(VIP), China National Knowledge Infrastructure(CNKI), Digital Journal Full-text Database(CHINAINFO), PubMed and Web of Science from January 1990 to October 2010.Combined OR values and 95% CI were calculated with Review Manager 5.0. Results   18 eligible studies were included in the metaanalysis, among which 11 studies(907 cases and 1,978 controls) on maternal MTR A2756G polymorphism, and 11 (1123 casesand 1700 controls) on maternal MTRR A66G polymorphism. Statistical analysis of the combined data showed that there was no significant association between maternal MTR A2756G polymorphism and the risk of NTDs, while there were significant associations between maternal MTRR A66G polymorphism and the risk of NTDs in GG/AG vs. AA, GG vs. AA, AG vs. AA, GG vs. AG/AA and G vs. A genetic models, and their pooled OR values and 95% CI were 1.89(1.282.78), 1.68(1.312.16), 1.77(1.18-2.66), 1.28(1.06-1.55) and 1.35(1.12-1.63), respectively. Conclusion   Maternal MTRR A66G polymorphism is a risk factor for NTDs.

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    Signal quality assessment of ECG in the intensive care unit
    LI Qiao,YU Meng-sun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1997)      PDF(pc) (388KB)(1893)       Save
    Objective: To develop an algorithm of signal quality assessment of ECG in the intensive care unit. Methods: The signal quality index (SQI) was obtained by analyzing the morphological and statistical characteristics of each waveform and their relationships. The heart rate was robustly estimated based on SQI. The algorithm was evaluated using more than 6?000 hours of simultaneously acquired ECG from a 437patient subset of the MultiParameter Intelligent Monitoring for Intensive Care II database and adding real ECG noises. Results: The SQI was decreased along with a decrease of the signal noise ratio. The SQI had high-positive correlation with the QRS detective sensitivity and positive predictivity. This method provided an accurate HR estimation even in the presence of high levels of persistent noises and artifacts. Conclusion: The signal quality assessment algorithm could provide an objective estimation of ECG quality.
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    Shandong multi-center longitudinal cohort for health management: a brief introduction
    LIU Yafei, XING Ping, XU Xiuqin, YANG Shufang, LIU Yanxun, YUAN Zhongshang, XUE Fuzhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (6): 30-36.   DOI: 10.6040/j.issn.1671-7554.0.2017.376
    Abstract2025)      PDF(pc) (1245KB)(1890)       Save
    Objective To introduce the characteristics of diseases and main variables, and the objectives of Shandong Multi-center Longitudinal Cohort for Health Management. Methods Shandong Multi-center Longitudinal Cohort for Health Management is a prospective, dynamic and open cohort. Since 2004, we began to collect data, construct database, conduct follow-ups, and so on. So far the total number of cohort is about 100 million, the longest observation time is 12 years, and about 20% of data have merged with medical insurance disease outcome data and cause of death. The information collection methods include questionnaires, physical examinations and laboratory tests. Results A total of 76 368 people were enrolled in this study, including 43 818 males and 32 550 females. The cumulative risk of hypertension, diabetes, stroke, and coronary heart disease is 49.40%, 23.98%, 4.74% and 6.82%, respectively. Conclusion Shandong Multi-center Longitudinal Cohort for Health Management was established in order to explore the roles of various factors in the development of chronic diseases, to construct the risk assessment models, and to provide a scientific basis for interventions.
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    Tumescent technique in multi-position liposuction
    CAI Jing-long,ZHU Gui-ying,DONG Hong,JIANG Du-yin,XU Bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1583)      PDF(pc) (332KB)(1862)       Save
    Objective: To study the clinical effect of tumescent technique in multi-position liposuction. Methods: Tumescent liposuction technique was used on 1200 different sites of 500 cases. The pre- and post-operative treatment, the tumescent liquid amount, the usage of microcannula in the superficial fat layer, the lidocaine dosage, instruments and operation methods, and the maintenance of body fluid equilibrium were all well controlled. Results: There was good efficiency such as fewer complications, obviously shrinking skins and smooth operation areas. Conclusion: Tumescent technique can meet the need of multi-position and large-volume liposuction. It is a safe and effective body sculpture technique.
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    Factor analysis of the simplified coping style questionnaire
    WANG Duanwei1,2, ZHANG Jingxuan2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2014, 52 (3): 96-100.   DOI: 10.6040/j.issn.1671-7554.0.2013.380
    Abstract2637)      PDF(pc) (926KB)(1841)       Save

     Objective  To explore the internal structure of the simplified coping style questionnaire through factor analysis, to analyze the relationship between the factors and the state of mental health, and to compare them with the two dimensional factor models of SCSQ. Methods  Stratified multistage cluster randomization was adopted to randomly select 23987 individuals ≥ 18 years of age from 20 urban neighborhoods (from 10 sub-districts in 5 urban districts) and 60 rural villages (from 30 rural townships in 15 counties) from 5 prefecture-level cities of Shandong Province. These subjects were assessed by general healthy questionnaire and simplified coping style questionnaire, and 22 718 persons actually completed questionnaires. Epidata 3.1 and SPSS 18.0 were applied to conduct statistical analysis. Results  The 20 items of the SCSQ were divided into 5 factors, which included active problem-focused coping styles (factor Ⅰ), active emotion-focused coping styles (factor Ⅱ), passive emotion-focused coping styles (factor Ⅲ), passive problem-focused coping styles (factor Ⅳ) and psychological counseling (factor Ⅴ). Every factor score of five dimensional factor models and two dimensional factor models were significantly related with the state of mental health, and every factor score of the different mental health state had significant difference. Conclusion  Compared with the two dimensional factor models, the five dimensional factor models can reflect the internal structure of SCSQ in multiple dimensions, and the mental health state of different population.

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    The electron flexible cystourethroscope combined with a fascia
    dilator for simple urethral stricture
    YUAN Hang, JIN Xun-bo, SUN Peng, LI Peng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (6): 101-.  
    Abstract1389)      PDF(pc) (1457KB)(1802)       Save

    Objective   To investigate the safety and efficacy of the electron flexible cystourethroscope combined with a fascia dilator to treat simple urethral stricture. Methods   80 patients with simple urethral stricture confirmed by physical examination and radiology underwent urethral dilation with the electron flexible cystourethroscope combined with a fascia dilator in our center. The outcomes were retrospectively analyzed in accordance with the stricture pattern, catheterization duration time and prognosis. Results   78 cases were successfully performed with urethral dilation without serious complications. The median time was 9min (7 to 12min). 64 patients were followed up (4 to 24 months). And 8 patients underwent the photo-selective vaporization of urethral stricture due to stubborn restenosis and personal delay of treatment. Conclusion   The electron flexible cystourethroscope combined with a fascia dilator in the treatment of simple urethral stricture demonstrates satisfactory safety, briefness and efficacy.

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    A study of happiness, stress and mental resilience of elderly of different ages
    JIANG Hong, XU Jingjing, WANG Rui, YI Xiangren, ZHOU Yaru, PAN Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (9): 11-16.   DOI: 10.6040/j.issn.1671-7554.0.2016.1415
    Abstract2470)      PDF(pc) (1031KB)(1800)       Save
    Objective To explore the happiness, stress and mental resilience of elderly of different ages. Methods A total of 622 elderly aged over 60 years who lived in the same community were investigated with the Face Scale, Perceived Stress Scale(PSS)and Connor-Davidson Resilience Scale(CD-RISC). Results The happiness of elderly displayed dynamic changes with aging, the 60- year group had the highest happiness, the 65- year group had declining happiness, and the 70- year group had the lowest happiness, which then remained at a low level with aging. The 80- year group had the lowest stress compared with other groups. The 60- year group and 65- year group had significantly higher scores of CD-RISC than the 80- year group and >85-year group. Elderly with good physical health, living with spouse and good income had high level of happiness. The divorced group had the highest stress score, and the moderate-income group had the best mental resilience. Age, marital status, income, physical health and mental resilience were significantly 山 东 大 学 学 报 (医 学 版)55卷9期 -江虹,等.不同年龄阶段老年人的幸福感、心理压力与心理弹性研究 \=-correlated with happiness, among which mental resilience had the greatest effect. Age, income, physical health and stress were significantly related to mental resilience, among which stress had the greatest effect. Marital status, physical health and mental resilience were the predicators of happiness. Gender, physical health and stress were the predicators of mental resilience. Conclusion Happiness of elderly changes dynamicly with aging. Elderly with higher mental resilience have higher level of happiness. Stress and physical health can reduce resilience, which influences the level of happiness.
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    Evaluation of the new method for ultrasound measurement of fetal lateral cerebral ventricle
    LI Yang1, TAO Guo-wei2, LIU Shao-ping2, MA Zhe2, ZHI Rui-juan2, WANG Yin2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (8): 82-84.  
    Abstract1124)      PDF(pc) (1463KB)(1798)       Save

    Objective   To evaluate a new method —the Guibaud method for ultrasound measurement of the fetal lateral cerebral ventricle. Methods   One hundred and forty  fetuses’ lateral ventricles width were  measured by the routine method and the Guibaud method. The Bland-Altman statistical method was used to compare the consistency between the two methods.30 fetuses were randomly selected to evaluate the repeatability and reproducibility. Results   The Guibaud method had high consistency with the routine method. 98.6% (138/140) of the points were in the 95% limits of consistency (-0.46,0.73);the mean difference was 0.13cm and the maximum absolute value was 0.7cm.  The repeatability and reproducibility of the Guibaud method was better(P>0.05). Conclusion   The Guibaud method has high consistency with the routine method.The standard planar is simple and stable to obtain. The repeatability and the reproducibility is better. All of these improve the accuracy of the measurement. 

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    Improvement of the scoring system on human oocytes during maturation in vitro
    YANG Yong-jie,SUN Shu-yu,MA Shui-ying,JIANG Jing-jing,LI Mei,HU Jing-mei,LI Yuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1441)      PDF(pc) (439KB)(1776)       Save
    To study the feasibility of the improved scoring system by evaluating oocytes cultured in vitro, and to discuss the relationship between developmental potential of human immature oocytes and their granulasa cells. Methods Cumulus enclosed oocyte complexes (COCs) were cultured, evaluated and fertilized. The IVM oocytes were morphologically classified into two groups based on the improved scoring standard. One group was of high quality and the other group was of poor quality. Granulosa cell apoptosis was detected by agarose gel electrophoresis. Results The immature oocyte scoring system was improved. The maturation rate,cleavage rate and high quality embryos of high quality oocytes were better than those of poor quality oocytes. DNA of granulosa cells distilled from poor quality oocytes was discovered as DNA ladder,while that from high quality ones was not. ConclusionThe improved scoring standard is feasible in clinical practice.
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    Effects of anti-epileptic drugs on the blood levels of homocysteine, folate and vitaminB12
    WANG Xiao-hua,ZAHNG Tong-xia,ZHAO Xiu-he,Gao Jing,LIU Xue-wu,CHI Zhao-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)   
    Abstract1763)      PDF(pc) (227KB)(1724)       Save
    Objective: To investigate the effect of antiepileptic drugs(AEDs) on the blood levels of homocysteine, folate and vitaminB12. Methods: There were 3 groups tested: ①88 epipleptic patients, taking a single AEDs; ②epileptic control group, 30 epileptic patients not taking AEDs; ③normal control group, 40 healthy controls. The levels of homocysteine, folate and vitaminB12 were determined by a fluorescence quantity method and an electrochemistry radiation immunity. Results: In epileptic patients taking a single carbamazepine (CBZ) and phenytoin(PHT), the levels of homocysteine were significantly higher and the levels of folate were significantly lower than those of the control group(P<0.001). The levels of homocysteine, folate and vitaminB12 were not significantly changed in patients taking valproic acid(VPA)(P>0.05). Conclusions: Homocysteine levels of epileptic patients taking CBZ and PHT were significantly increased whereas the folate levels of epileptic patients taking CBZ and PHT were signifacantly decreased. An adequate dosage of folate and vitaminB12 for epileptic patients is helpful in preventing angiocardiopathy and cerebrovascular diseases.
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    Implantation of 125I seeds for treatment of local bronchogenic carcinoma
    ZENG Yiming, LIN Yanping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2017, 55 (4): 1-6.   DOI: 10.6040/j.issn.1671-7554.0.2016.1665
    Abstract1671)      PDF(pc) (1114KB)(1695)       Save
    Lung cancer is a kind of malignant tumor with high morbidity and mortality in China. In recent years, with the improvement of medical level and the development of science and technology, interventional therapy plays an important role in lung cancer trerapy. More and more studies have shown that implantation of 125I radioactive seeds for treatment of patients with advanced lung cancer has achieved good results. But for the lung cancer at early stage, the effect of implantation of 125I radioactive seeds needs to be further studied.
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    Brain tumor image segmentation based on deep learning techniques
    Ju LIU,Qiang WU,Luyue YU,Fengming LIN
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 42-49, 73.   DOI: 10.6040/j.issn.1671-7554.0.2020.0391
    Abstract3866)   HTML256)    PDF(pc) (6300KB)(1675)       Save

    Artificial intelligence technology is widely applied in the field of computer vision and deep learning. Image segmentation technology based on deep learning is essential in industries such as autonomous driving, drones, and clinical diagnosis and treatment. This paper reviews the methods of brain tumor image segmentation, including the traditional methods of image segmentation and methods based on deep learning and some typical methods. The paper also compares our research advances with the typical methods and discusses future research directions and challenges.

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    Assessing the general mental health of college students:psychometric properties of GHQ-28
    CHEN Cui, ZHANG Hongjing, JIANG Hong, LI Wenjie, Lv Li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (3): 159-162.  
    Abstract9113)      PDF(pc) (330KB)(1673)       Save

    Objective   To check the psychometric properties of GHQ-28 in college students. Methods   Three questionnaires, GHQ-28, SAS, and SDS, were used to assess 248 college students. Then the item-analysis was used to analyze each item of the GHQ-28,  the Cronbach′s α of the GHQ-28 was checked,  factor analysis was used to check the structure validity of the GHQ-28 and the SAS and SDS was applied to check the criterion validity of the GHQ-28. Results    The correlations between each item of the GHQ-28 and its scales were positive (P<0.01). The reliabilities of the whole scale and the part scales were 0.872, 0.767, 0.667, 0.756 and 0.700. The GHQ28 contains four factors, and the aberration rate of them was 48.381% of the total. The GHQ-28 factors′ levels of students with high scores in SAS were higher than those low in SAS, as well as in SDS (P<0.001). The correlations of the GHQ-28 and SAS, SDS were 0.707 and 0.749. Conclusion    The GHQ-28 has good reliability and validity when it measures the general mental health of college students.

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    Long-term prognosis of ocular myasthenia gravis in children and its related factors
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (5): 93-96.  
    Abstract2192)      PDF(pc) (356KB)(1643)       Save

    Objective  To explore long-term prognosis of ocular myasthenia gravis(OMG) in children and its related factors. Method  s88 patients aged between 2 and 14 years at onset were selected for this follow-up survey, and they were primarily diagnosed as having OMG. The follow-up time was 5 years to 10 years. At the end of the follow-up, the long-term prognosis of OMG was evaluated and its related factors were analyzed by the Logistic regression. Results  At the end of the follow-up,13 patients (14.77%) were asymptomatic after taking drugs for about two years;59 patients (67.05%) remained having OMG throughout the follow-up duration, including 26 recrudescent patients after drug withdrawal;16 patients (18.18%) converted into generalized myasthenia gravis, most of whom (9/16, 56.25%) occurred within 1 year after onset. The long-term prognosis of OMG had a significant correlation with acetylcholine receptor (AchR) antibody and thymopathy, including thymoma and thymic hyperplasia(P<0.05). Conclusion  Prognosis of OMG in children has some distinctive characteristics and the probability of OMG converting into generalized myasthenia gravis is lower than that in adults. Several factors, such as AchR antibody and thymopathy, may have an impact on the risk of OMG developing into generalized disease in children.

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    Clinical observation on intravesical instillation of sodium hyaluronate for treatment of non-bacterial cystitis
    ZHANG De-qing, ZHU Yao-feng, MENG Hui, WANG Jun, LI Yan, SHI Ben-kang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2013, 51 (1): 79-82.  
    Abstract1582)      PDF(pc) (891KB)(1626)       Save

    Objective   To observe the clinical efficacy and safety on intravesical instillation of sodium hyaluronate for treatment of non-bacterial cystitis. Methods   Totally 28 patients with non-bacterial cystitis were included. They received intravesical instillation(sodium hyaluronate solution of 50ml/40mg) once a week for 4 weeks, then once every two weeks for the next 4 weeks and at last once a month for the next 2 months. The follow-up visited was performed once every two weeks, ended at the 16th week. Evaluate the efficacy and safety of the sodium hyaluronate according to patients’ symptom assessment scores(VAS, LUTS) and the micturition diary. Results   28 patients all completed the instillation and follow-up. At the 16th week, the VAS score dropped from 6.86±1.74 to 2.71±0.90(P<0.001), the LUTS score dropped from 41.07±11.96 to 23.25±6.07(P<0.001) and the mean BC(bladder capacity) rose from (106.71±22.65)mL to (163.93±32.00)mL(P<0.001). Conclusion   The short-term curative effect and safety of intravesical instillation of sodium hyaluronate to treat the non-bacterial cystitis is worth affirmation. It can significantly relieve symptoms in order to improve the quality of life.

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    Mitochondrial dysfunction and related brain diseases
    Chuanzhu YAN,Wei WANG,Kunqian JI,Yuying ZHAO
    Journal of Shandong University (Health Sciences)    2020, 1 (8): 34-41.   DOI: 10.6040/j.issn.1671-7554.0.2020.0725
    Abstract1721)   HTML69)    PDF(pc) (1510KB)(1619)       Save

    Mitochondria provides most of the energy for the body through oxidative phosphorylation (OXPHOS) and participates in various physiological and biochemical processes such as cell signal transduction, inflammatory response and apoptosis. Brain is very sensitive to energy change. Mitochondrial dysfunction is associated with various disorders including genetic and metabolic diseases, neurodegenerative diseases, psychological diseases and brain aging via multiple pathways. At present, there is no specific treatment plan for mitochondrial encephalopathy. Research on mitochondrial gene targeted therapy is expected to break through the treatment bottleneck of mitochondrial diseases and brings hope to patients

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