Abstract:

To explore the effects of survivin antisense oligonucleotide(ASODN) on apoptosis, growth and sensitivity to vinorelbine in the human breast cancer cell MCF7. MethodsSurvivinASODN was transfected into the MCF7 cells mediated by the Lip reagent. Expression of survivin mRNA was determined by RTPCR and of survivin protein by Western blot. Apoptosis rate and cell cycle changes in antisense oligonucleotide(ASODN) transfected cells were detected by FCM. Cell proliferation and chemosensitivity to vinorelbine in MCF7 were determined by the MTT assay. The effect of survivin antisense oligonucleotide(ASODN) in inducing apoptosis of MCF7 cells was observed under an electron microscope. ResultsSurvivin ASODNLip compound efficiently downregulated the mRNA and protein expressions of survivin in MCF7 cell lines in a concentrationdependent manner. The cell cycle was arrested at the G2/M phase， the cell apoptosis rate was increased obviously(P＜0.05), and growth inhibition was evidently increased in antisense oligonucleotide(ASODN) transfected cells（P＜0.05）. Terminal apoptosis changes were observed under an electron microscope after antisense oligonucleotide(ASODN) transfection. The 50% inhibitory concentration(IC50) of vinorelbine was significantly lower for 600?nmol/L antisense oligonucleotide(ASODN) transfected MCF7 cells than for control cells ［(3.7±0.42)μg/mL ConclusionSurvivin

Key words: Breast neoplasms, Survivin oligoribonucleotides,antisense, Cell proliferation, Apoptosis, Vinorelbine