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Table of Content

      
    10 November 2012
    Volume 50 Issue 11
    Articles
    Effects of duodenal jejunal bypass on the expression of GLP-1R in
    the enteric nervous system of type 2 diabetic rats
    ZHANG Xiu-rong1,2, XU Ji-ma1,3, YAN Qing-tao1,4, JI Wan-sheng3, FANG Chun-yan1,2, WANG Lin1,2, ZHAO Ting-kun1,2, GAO Zhi-qin1,5, QU Mei-hua1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  1-5. 
    Abstract ( 793 )   PDF (1710KB) ( 366 )   Save
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    Objective   To study the effects of duodenal jejunal bypass(DJB) on the expression of Glucagon-like peptide-1receptor (GLP-1R) in the enteric nervous system of type 2 diabetic(T2DM)rats, and to explore the relation-ship between GLP-1R expression, GLP-1 secretion and blood glucose. Methods   Wistar rats were randomly divided into four groups: Wistar control group (W-C), Wistar DJB surgery group (W-DJB), T2DM control group(T2DM-C), T2DM DJB surgery group (T2DM-DJB), 8 rats in each group. Fasting blood glucose and glucagon-like peptide-1 (GLP-1) were detected before DJB operation and 1week, 4 weeks, 8 weeks after DJB operation. Meanwhile, fasting insulin before DJB operation and 8 weeks after DJB operation was also detected. After 8 weeks, the rats were sacrificed and the expression of GLP-1R in the enteric nervous was studied by RT-PCR and immunofluorescence. Results   The number of GLP-1R positive cells in the T2DM-DJB rats enteric nervous system was 15.83±2.23, it increased significantly compared to that of the T2DM-C rats which was 8.50±2.67(P<0.01). The expression of GLP-1R mRNA in the T2DM-DJB rats enteric nervous system was 0.67±0.08, it also increased significantly compared to that of the T2DM-C rats which was 0.42±0.03(P<0.01). The fasting blood glucose and fasting insulin of T2DMDJB rats decreased after DJB surgery(P<0.01), and the fasting serum GLP-1 increased after DJB surgery (P<0.01). GLP-1R expression level, fasting blood glucose, fasting insulin and fasting serum GLP-1 of the W-DJB rats had no significant difference after DJB surgery compared to those of the W-C rats(P>0.05). Conclusion   DJB surgery can significantly decrease the fasting blood glucose of T2DM rats, but it has no obvious effect in normal rats.

    The influence of PDCD4 gene to the differentiation of
    T lymphocytes subsets
    LIU Xiang-lan, JIANG Yang, WANG Qun, ZHU Fa-liang, DONG Zhao-jing, GUO Chun, ZHANG Li-ning
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  6. 
    Abstract ( 960 )   PDF (3977KB) ( 428 )   Save
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    Objective   To investigate the influence of PDCD4 gene to the differentiation of T lymphocyte subsets. Methods   Detect the expression differences of CD8+T, CD4+T and its subsets such as Th1、Th2, Th17 and CD25+Foxp3+Treg in the splenocytes and lymph nodes of pdcd4-/- mice and C57BL/6 mice by flow cytometry. And analyse the ratio change of the lymphocyte subsets. Results   Compared with that of the C57BL/6 mice, the number of CD8+T cells decreased in the pdcd4-/- mice(P<0.05). CD4+T and Th1 cells also showed a descending tendency but had no statistic significance(P>0.05). The proportion of CD4+CD25+Foxp3+Treg significantly increased in the pdcd4-/- mice (P<0.05).  Conclusion   PDCD4 can influence the differentiation of partial T lymphocyte subsets, the low expression of CD8+T cells as well as high proportion of CD4+CD25+Foxp3+Treg in the pdcd4-/- mice suggest that PDCD4 gene may play a role in the immune regulation.However, PDCD4 gene has no significant influence on the differentiation of CD4+T and Th1、Th2 and Th17 cells.

    Pathologic study on improvement of Alzheimer’s disease mice
    treated with ethanol soluble extract of polygala
    YAO Yun-yi1, SUN Li2, XUE Jian-hong3, HOU Qian4, LIU Xiao-qun5, ZHOU Xiao-li5, YAN Hong-ying6, HONG Hua-rong7, YU Guo-qiang8, CHEN Fang1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  11. 
    Abstract ( 1215 )   PDF (3487KB) ( 378 )   Save
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    Objective   To determine the pathologic effects of ethanol soluble extract of polygala on the growth of new born neurons in hippocampus and the formation of neurofibrillary tangles(NFT) and senior plaques(SP) in both normal and Alzheimer′s disease(AD) mice,together with the detection of its effects on oxidative stress and Aβ40, Aβ42 in the mice brain in normal and AD mice. Methods   9-month mice devided by sex were used to generate the AD mice as the pathological group together with normal mice at the same age as the normal group, both contain female and male mice. Placebo group (control) and drug treatment group were divided in both groups, totally 4 groups. Immunohistochemistry(IHC) was used to detect the growth of new born neurons in SGZ. Thioflavin S staining was used to detect the NFT and SP in the cerebral cortex of hippocampus in mice. 8-isoprostane and Aβ40,Aβ42 were detected by ELISA. Results   Compared with that of the normal group, the growth of new born neurons became slow and the formation of NFT and SP in the cerebral cortex of hippocampus increased in the pathological group. Compared with that of the placebo group, the growth of new born neurons became better and the formation of NFT and SP in the cerebral cortex of hippocampus decreased in the drug treatment group. The effect was more obvious in the female group than in the male group. 8-isoprostane and Aβ40, Aβ42 were down-regulated in AD mice obviously. Conclusion   The ethanol soluble extract of polygala can promote the growth of new born neurons in hippocampus and reduce the formation of NFT and SP in the cerebral cortex of hippocampus. It can also reduce OS and Aβ40, Aβ42, ameliorate the pathological defects in AD brain,and keep the function of normal neurons. Polygala may be one of the potential drugs to treat AD.

    The combination between vasodilator-stimulated
    phosphoprotein and microtubule protein
    TAO Yan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  18. 
    Abstract ( 937 )   PDF (2286KB) ( 325 )   Save
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    Objective     To investigate the relationship between vasodilator-stimulated phosphoprotein (VASP), serine 157/serine 239 phosphorylated VASP (pVASP S157/p-VASP S239) and α-Tubulin, and to confirm VASP as a new microtubule-associated protein (MAP).  Methods    The co-localization of p-VASP S157/p-VASP S239 and α-Tubulin in Hela cells were analyzed by the immunofluorescencent assay. Co-immunoprecipitation assay, Microtubule pelleting assay and Western blot assay were applied to detect the combination between VASP/phosphor-VASP and α-Tubulin.  Results   VASP and p-VASP S157 could bind with α-Tubulin. p-VASP S239 did not co-localize with α-Tubulin during the whole cell cycle.  Conclusion    VASP is a new microtubule-associated protein, and the serine 157 phosphorylation of the protein is involved in the association between VASP and α-Tubulin.

    Effects of basic fibroblast growth factor on cortex of parietal lobe and
    middle cerebral artery in atherosclerotic model rats 
    ZHU Jun-de1,2, WANG Gui-xue2, YU Yan1, YU Zi-jiang1, XIAO Zhao-lun1, WANG Yu-lin1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  23. 
    Abstract ( 659 )   PDF (3081KB) ( 383 )   Save
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    Objective   To study the protective effects of basic fibroblast growth factor (bFGF) on cortex of parietal lobe and M1 segment of middle cerebral artery in atherosclerosis (AS) model rats. Methods   A total of thirty male adult Wister rats were randomly divided into the normal control group, the AS model group and the bFGF treatment group. Except for the control group, rats of the other two groups were injected with a single dose of vitamin D3 (6×104IU/kg) and loaded with high fat diet for six weeks continuously. The bFGF was injection into the abdominal cavity after six weeks in the bFGF treatment group at 9.5μg/kg for two weeks continuously, and an identical volume saline was given to the AS model group and the normal control group. After eight weeks, all the rats were sacrificed. Then, the serum total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein were respectively assayed, and the colorimetric method was used to detect the level of superoxide dismutase (SOD), Nitric-Oxide (NO) and the content of Malondialdehyde (MAD) in the brain tissues. The cholesterol and Caspase-3 content of the M1 segment of middle cerebral artery were respectively detected by the highperformance liquid chromatography and Western blot. As well the pathological lesion of the M1 segment of middle cerebral artery and cortex of parietal lobe were observed under a light microscopy and quantitative analysis was performed by cell morphometric technique. Results   The early AS plaques were presented after six weeks by hyper lipid foods. Compared with those of the AS model group, the level of serum lipid, the content of cholesterol and Caspase-3 expression in the bFGF treatment group obviously decreased (P<0.05); The content of MAD and activity of SOD and NO also obviously decreased in the brain tissues (P<0.05); The pathologic injury of both the brain tissue and the M1 segment of middle cerebral artery decreased (P<0.05). Conclusion   bFGF may play an important role in antagonizing the brain artery and cortex injuries not only by regulating the oxidizing ability and the level of blood lipid, but also by decreasing the pathologic injuries of both the brain tissue and artery.

    Expressions of NF-κB and IL-6 around the hematoma and their correlations
    with cerebral edema after intracerebral hemorrhage in rats
    ZHOU Hong-xia1, LOU Ning2, WEI Zi-feng1, ZHANG Yu-xin1, MENG Ling-li1, ZHANG Zuo-feng1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  30-33. 
    Abstract ( 936 )   PDF (1829KB) ( 504 )   Save
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    Objective   To investigate the expressions of NF-κB and IL-6 around the hematoma and their correlations with cerebral edema in rat′s brain after experimental intracerebral hemorrhage(ICH). Methods   120 SD rats were randomly divided into 1 sham-operated group and 5 ICH model groups(20 in each group). The intracerebral hemorrhage model was established by nonthrombogenic autologous blood injection in rats. The water content of brain(BWC)-was measured with dry/wet weight method. Histopathological changes were determined by Nissl staining. Locations of NF-κB and IL-6 proteins around the hematoma in cerebral tissues were detected by immunofluorescence. NF-κB and IL-6 expressions around the hematoma in cerebral tissues were detected by immunohistochemistry. Results   Nissl′s bodies of nerve cells in ICH model groups were obviously lost. NF-κB and IL-6 expressions in ICH model groups were significantly higher than those in sham-operated group(P<0.05). NF-κB and IL-6 appeared at 6h, then gradually increased and reached a maximum at 3d. Significant differences existed between ICH model groups and sham-operated group(P<0.05). There were positive correlations between the expressions of NF-κB and IL-6 and the cerebral water content(P<0.05). Conclusion   High expressions of NF-κB in the rats of ICH model groups show that activatedNF-κB can aggravate inflammatory reactions by inducing cytokine IL-6 expression, and take part in the pathological process of brain edema.

    Screening and biological characteristics study of human
    single chain Fv antibody against prostatic cancer
    ZHENG Gui-xi, WANG Chuan-xin, ZHANG Xin, LI Wei, DU Lu-tao, LI Juan, LIU Hui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  34-38. 
    Abstract ( 914 )   PDF (2919KB) ( 371 )   Save
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    Objective   To screen the soluble ScFv antibodies which were specific for human prostatic cancer from the successfully constructed phage antibody library and analyse their biological characteristics. Methods   Normal human noncancerous epithelial line BPH was used to deplete the phage library of nonspecific binders. Supernatant containing depleted phage library was then incubated with prostate cancer cells DU145. The phage library was enriched by three cycles of binding, elution and amplification.The prostate cancer cells DU145 was used to screen the positive clones with high affinity using FACS. The screening postive clone was sub-cloned and infected by TG1 to get the soluble ScFv antibody. The biological characteristics of ScFv were identified by the method of FACS and immunofluorescence. Results   19 phage antibodies were gotten with the antigens of DU145. C11 had the highest affinity with DU145 cells among all the above antibodies. C11 ScFv monoclonal antibody was expressed and purified after sub-cloning. The results of FACS and immunofluorescence both indicated that C11 ScFv could bind to DU145 and PC3 cells but could not bind to BPH cells. Conclusions   The soluble ScFv antibody which has high affinity with prostatic cancer cell line is successfully producted by phage antibody display technology. This may be useful for futher study of target therapy of antibody.

    Differences in the expression of Cx45 in brain artery between
    spontaneously hypertensive rats and Wistar rats
    LI Xin-zhi1,2, SI Jun-qiang1,3, CHEN Xin-yan1,3, PANG Li-juan1, LI Li1,3, WEI Li-li1,3, MA Ke-tao1,3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  39-42. 
    Abstract ( 846 )   PDF (1343KB) ( 299 )   Save
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    Objective   To investigate the change of gap junction in brain artery of spontaneous hypertension(SH) rats and its relationship with hypertension. Methods   Whole-cell patchclamp, quantitative RT-PCR and Western blot technique were used to investigate the difference in electrophysiological properties of gap junction and expressions of connexin 45 (Cx45) at mRNA and protein levels in brain artery between SH rats(n=30) and Wistar rats(n=30). Results   ① As detected by whole-cell patch-clamp technique, the membrane capacitance and membrane conductance of vascular smooth muscle cells in brain artery from SH rats were significantly higher than that from normotensive Wistar rats (P<0.05), suggesting that gap junctions coupling among VSMCs were enhanced in SH rats. ② As detected by quantitative RT-PCR technology, the level of Cx45 mRNA expression in brain artery from SH rats was significantly increased compared with that from normotensive Wistar rats (P<0.05). ③ As detected by Werstern blot technique, the expression of Cx45 protein was significantly elevated in brain artery from SH rats compared with that from normotensive Wistar rats(P<0.01). Conclusion   The upregulation of Cx45 expression can enhance gap junctional communication in vascular smooth muscle cells of brain artery from SH rats to ensure the synchronous contraction of brain artery.

    TWEAK promotes expressions of collagen I and matrix metalloproteinase1
    in rat cardiac fibroblasts via P38MAPK pathway
    WANG Qi-lei1, REN Man-yi2, WANG De-jin1, XU Dong-ling1, DU Yi-meng1, WANG Xu-ping3, SUI Shu-jian1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  43-47. 
    Abstract ( 966 )   PDF (1648KB) ( 361 )   Save
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    Objective   To investigate the mechanism and effects of tumor necrosis factor-like inducer of apoptosis (TWEAK) on expressions of collagen I and matrix metalloproteinase1(MMP-1) in cardiac fibroblasts(CFs). Methods   Cultured CFs from neonatal Wistar rats were treated with the recombinant human TWEAK and the P38MAPK specific inhibitor(SB203580). CFs were divided into 3 groups: ① the control group: CFs were cultured without any stimulus; ② the TWEAK group: CFs were incubated with 100ng/mL TWEAK; ③ the TWEAK+SB203580 group: CFs were incubated with 100ng/mL TWEAK+SB203580. The CFs proliferation was examined by MTT, and expressions of collagen Ⅰ-and phosphor-P38MAPK protein were detected by Western blot. qRT-PCR was used to detect the collagen Ⅰ mRNA level. ELASA was used to detect the concentration of MMP1 in CFs cell-culture supernatant. Results   TWEAK promoted the proliferation of the CFs and expressions of collagen Ⅰ and phosphor-P38MAPK protein. The expressions of collagen Ⅰ mRNA and MCP-1 were up regulated due to TWEAK. P38MAPK specific inhibitor (SB203580) could inhibit the proliferation of CFs. P38MAPK specific inhibitor (SB203580) could partially inhibit expressions of collagens Ⅰ mRNA , collagens Ⅰ protein, phosphor-P38MAPK protein and MMP-1. Conclusion   TWEAK can promote expressions of collagen Ⅰ and MMP-1 in rat cardiac fibroblasts via P38MAPK pathway.

    Experimental study for the repair of knee-joint cartilage defects by
    using nano-tricalcium phosphate(N-TCP)artificial bone and
    bone marrow mesenchymal stem cells (BMSCs)
    WU Jun-cheng1,2, L Ren-rong1, LI Long1, ZHOU Sheng-ru1, LIN Ling-ling1,NIU Jing-jing1, ZHANG Lin-feng1, XU Guang-qi1, HUO Ran1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  48-52. 
    Abstract ( 871 )   PDF (2561KB) ( 563 )   Save
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    Objective   To examine the effects of simultaneous application of Nano-Tricalcium phosphate(N-TCP) artificial bone and bone marrow mesenchymal stem cells(BMSCs)plus the transforming growth factor-β(TGF-β) and insulin-growth factor-Ⅰ(IGF-Ⅰ) in repairing articular cartilage defects. Methods   MSCs isolated from the bone marrow of New Zealand white rabbits were cultured in vitro and induced to chondrocytes. Auricular cartilages of allogenic rabbits were firstly decellularized and then integrated with N-TCP composite artificial bone in forming N-TCP-DC matrix scaffolds. Then the chondrocytes were loaded into the scaffolds. TGF-βand IGF-Ⅰwere added to repair the knee-joint cartilage defects. In experimental animals, 18 knee-joint articular cartilage defects were created. the defects were treated using N-TCP-DC matrix scaffolds with TGF-βand IGF-Ⅰ(n=8) in comparison to controls of N-TCP-DC matrix scaffolds alone(n=6) and no engineered cartilage implants at all(n=4). Results   BMSCs grew stably, reproduced vigorously and could be induced into chondrocytes in vitro. The auricular cartilages were completely decellularized and the cartilage lacunae arrayed orderly. Around the 20th week, with the white translucent scaffold filling the articular cartilage defects, the newly formed cartilage in the experimental group connected well with the normal cartilage revealing similar color with that of the normal cartilage.Conclusion   Being a porous structure, N-TCP-DC matrix scaffold is significant in regard to its rapid degradation ability, good histocompatibility and cell adhesive ability, making it a promising candidate for the cartilage tissue-engineering applications.

    Effects of TMPyP4-PDT on human cervical cancer cells
    DING Bai-juan, ZHANG You-zhong, L Chang-shuai, JIANG Kan, LIU Hong-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  53-57. 
    Abstract ( 1238 )   PDF (2359KB) ( 373 )   Save
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    Objective   To investigate the effect of photodynamic therapy mediated by meso-5,10,15,20-Tetrakis(N-methyl4-pyridyl) porphine (TMPyP4) on human cervical cancer HeLa cells in vitro. Methods   The proliferative activity of HeLa cells was assessed by Cell Counting Kit-8 (CCK8) assay, and the modality of TMPyP4-PDT treated cells were observed with light microscope. The apoptosis was examined by Annexin-V-FITC/PI double dye kit, and the expressions of c-myc mRNA and hTERC mRNA of the cell lines were tested by RT-PCR. Results   At the certain range of the concentration, the inhibition rate and induced cell apoptosis rate mediated by TMPyP4-PDT increased with the increase of the dosage and the light energy. The necrosis and apoptosis cells were observed with light microscope. The expressions of c-myc and hTERC mRNA were decreased. Conclusion   TMPyP4-PDT has a distinctive killing effect on HeLa cells, which shows a concentration-dependent manner and an energy-dependent manner. The possible mechanism may be related with down-regulating the expressions of c-myc mRNA and hTERC mRNA.

    Integrin β1 is involved in trans-differentiation of len epithelial
    cells induced by transforming growth factor-β2 in vitro
    ZHU Yu-guang, ZHU Yan, WANG Jie, ZHONG Ying-ying, DU Xiao-nan, ZHANG Rong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  58. 
    Abstract ( 892 )   PDF (1723KB) ( 380 )   Save
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    Objective   To investigate the effect of integrin β1 in trans-differentiation of human len epithelial cells(HLECs) induced by transforming growth factor-β2 (TGF-β2) in vitro. Methods   HLECs were cultured and passaged. The 3rd generation of HLECs were treated with TGF-β2. Expressions of integrin β1, E-cadherin,α-SMA and F-actin in the cells were analyzed with immunofluorescent staining. Expressions of α-SMA mRNA and Ecadherin mRNA were detected by RTPCR. For some cells, disintegrin kistrin was pre-treated 1h followed by TGF-β2 incubation in 48h. Results   Expressions of integrin β1,α-SMA and F-actin were significantly increased after treatment of TGF-β2, while expression of E-cadherin was significantly decreased after treatment of TGF-β2. Disintegrin kistrin partly reversed TGF-β2 induced expressions o f a-SMA, F-actin and Ecadherin. Conclusion   TGF-β2 induced trans-differentiation of HLECs towards myofibroblasts. Integrin β1 was involved in initiation of the trans-differentiation induced by TGF-β2.

    Effects of Toutongning on expressions of c-fos and
    c-jun in the rat migrainous model
    DONG Ling-fen1, XING Yi2, SUN Ling-zhi3, LI Xu-yang1, ZHANG Xiao-dan1, LI Shu-ling1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  62. 
    Abstract ( 975 )   PDF (1024KB) ( 402 )   Save
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    Objective   To explore the effect of Toutongning on the expression of c-fos and c-jun in the migrainous model. Methods   60 Wistar rats, in both genders, were randomly divided into 6 groups: the normal saline(NS)group, the model group, the Flunarizine Hydrochloride group, the high dose of Toutongning group, mid dose group, and low dose group. The nitroglycerin migrainous animal model was constructed with the nitroglycerin (NTG)subcutaneous injection. The expression of c-fos and c-jun was detected with the immunohistochemical method. Results   c-fos and c-jun positive cells in the model group significantly increased compared with the NS group(P<0.01). The expression of c-fos and c-jun in the high-dose Toutongning group and Flunarizine Hydrochloride group decreased compared with the model group(P<0.01). The expression of c-fos and c-jun in the mid-dose Toutongning group decreased compared with the model group(P<0.05). The expression of c-fos in the low-dose Toutongning group decreased, but there was no significant difference between the model group and the low-dose Toutongning group. The c-jun positive cell number in the low-dose Toutongning group decreased, compared with the model group(P<0.05). Conclusion   Toutongning can lower the expression of c-fos and c-jun induced by nitroglycerin, and can inhibit the transmission of nociceptive pain information.

    Role of hemorrhagic fever with renal syndrome(HFRS)critical score in
    predicting the prognosis for patients with HFRS
    GUO Nan1, CUI Su-nan2, WANG Xiao-yan2, CUI Hai-bin1, WANG Ming-ming2, GONG Yan-xue1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  67-70. 
    Abstract ( 962 )   PDF (1062KB) ( 558 )   Save
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    Objective   To investigate the role of hemorrhagic fever with renal syndrome(HFRS)critical score in predicting the prognosis for patients with HFRS. Methods   A total of 120 patients diagnosed as HFRS were divided into the survival group (90 cases) and the death group (30 cases) according to the prognosis. HFRS critical score consisted of SIRS state, extravasation, plasma colloid osmotic pressure, platelet count and urinary protein. The results of HFRS critical score were compared with the SOFA score and the  SAPS Ⅱ score. Results   All of the five parameters of HFRS critical scores in the death group were significantly higher than those in the survival group. The score of HFRS critical score, SOFA scores and SAPS Ⅱ score in the death group were significantly higher than those in the survival group(P<0.01). The area under the curve (AUC) of SOFA score, SAPS Ⅱ scores and HFRS critical score was 0.704, 0.731 and 0.804 respectively. The Youden index of the HFRS critical scores was the highest (P<0.01) among all three scores . If HFRS critical score 10 was used as the cut off value, the specificity and the sensitivity for predicting the death risk for the patient in admit was respectively 77.4% and 78.8%. Conclusion   HFRS critical score, SOFA score, and SAPS Ⅱ score can all effectively predict prognosis for HFRS patients, among which the HFRS critical scores are more easy and convenient , and have the best predictive value.

    Effects of alcohol intake on coronary collateral circulation and
    serum level of VEGF in myocardial infarction patients
    ZHU Ye, SUN Yong-le, YUAN Hai-tao, ZHANG Xing-hua, ZHANG Yu-ying, TANG Yuan-sheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  71-74. 
    Abstract ( 832 )   PDF (1115KB) ( 277 )   Save
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    Objective   To investigate the effects of different amount of alcohol intake on coronary collateral circulation and serum levels of vascular endothelial growth factor(VEGF)in patients with myocardial infarction. Methods   103 cases with at least one major coronary vessel occlusion confirmed by the coronary angiography were collected, and their coronary collateral circulations were classified with the Rentrop scoring system. Patients’daily alcohol intake and other risk factors of coronary artery disease were collected with the questionnaire survey method. Patients were divided into three groups according to the amount of alcohol intake: the non-drinking group, the light-drinking group(≤40g per day) and the heavy-drinking group(>60g per day). Serum levels of VEGF were detected with ELISA. Results   Coronary collateral circulations in the light-drinking group were better than those of the non-drinking group(P<0.05) and the heavy-drinking group(P<0.01); the serum level of VEGF in the lightdrinking group was significantly higher than that of the non-drinking group(148.32±60.80 vs 113.53±61.82)pg/mL(P<0.05), while there was no significant difference in serum levels of VEGF between the light-drinking group and the heavy-drinking group(P>0.05). Conclusion   Light alcohol intake may benefit the collateral circulation formation in patients with myocardial infarction, which may be associated with the overexpression of VEGF.

    Clinical and neuroimaging features of patients with
    middle cerebral artery stenosis
    ZHANG Tong-xia1, YAN Chuan-zhu1, ZHAO Xiu-he1, CHI Ling-yi2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  75-78. 
    Abstract ( 867 )   PDF (2497KB) ( 524 )   Save
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    Objective   To study the clinical and neuroimaging features of patients with unilateral middle cerebral artery (MCA) stenosis and to investigate its mechanism. Methods   30 patients with unilateral MCA stenosis were screened by magnetic resonance angiography (MRA) and carotid ultrasound. Further examinations such as diffusionweighted imaging (DWI) and digital subtraction angiography (DSA)were performed and the relationship between the results and their clinical data was analysed. Results   Among the 30 patients, 23 had acute stroke, 5 had transient ischemic attack (TIA) and 2 were asymptomatic. Among the patients with acute stroke, 16 had multiple acute infarcts and 7 had single acute infarcts. Among the patients with TIA, 2 had multiple lacunars and the other 3 had no positive findings on DWI. 2 asymptomatic patients had no positive findings on DWI. Conclusion   Various stroke patterns may be present in the patients with MCA stenosis. The possible common stroke mechanism include artery-to-artery embolism, stem plaque strentching and hypopefusion resulting from stenosis. Individualized treatment should be formulated according to different pathogenesis.

    Expression and clinical significance of EP2 prostanoid receptor in
    colon mucosa of ulcerative colitis
    LI Mei1,2, GAO Yan-jing1, CUI Xiang-hua1, XU Yong-mei3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  79-83. 
    Abstract ( 1117 )   PDF (2150KB) ( 626 )   Save
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    Objective   To explore the relationship between EP2 and ulcerative colitis (UC) by detecting the expression of EP2 in mucosa of UC. Methods   27 patients with active UC, 11 patients with remissive UC and 23 normal control subjects were recruited into the trial. The ulcerative colitis disease activity index (UCAI) was used to calculate the pathological grade and disease stage. Expressions of EP2 were detected by the immunohistochemistry in colonic tissues of the patients and controls.  Results   The EP2 protein levels in colonic epithelial and inflammatory cells in the patients with active UC were higher compared with the patients with remissive UC and controls (P<0.01). In the patients with active UC, EP2 expressions were positively related to the pathological grade.  Conclusion   Expressions of EP2 were significantly up regulated in the colon  mucosa of active UC patients and positively correlated with the inflammation degree,which indicates EP2 may be a new target for the UC treatment.

    Expression of osteopontin in the colorectal
    adenoma-carcinoma sequence
    YU Hua-long1, HU San-yuan2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  84-87. 
    Abstract ( 936 )   PDF (1879KB) ( 412 )   Save
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    Objective   To explore the expression of osteopontin (OPN) in the colorectal adenoma-carcinoma sequence, and analyze the relationship with the epithelial dysplastic degree.  Methods   Expressions of OPN were detected by the immunohistochemistry in 60 cases with normal colorectal epithelia, 50 cases with adenoma and 60 cases with carcinoma. Results   During the progression from normal colorectal epithelia to the carcinoma, OPN expressions significantly increased. The positive rates of OPN expressions in normal epithelia, adenoma and carcinoma were 3.3%, 66% and 81.5%. OPN expressions were related with the dysplastic degree of epithelia.  Conclusion   During the adenoma-carcinoma sequence in the colorectum, OPN expression rates increase with the development of the epithelial dysplasia. OPN may play an important role in the malignant transformation.

    Effect of straight light beam greenlight PVP for the
    treatment of benign prostatic hyperplasia
    ZHONG Hao, LI Bo, WANG Jin, WEI Chun-xiao, JIN Xun-bo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  88. 
    Abstract ( 936 )   PDF (1424KB) ( 483 )   Save
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    Objective   To summarize the method, effect and safety of 160W straight light beam greenlight photoselective vaporization of prostate (PVP)in the treatment of benign prostate hyperplasia(BPH), and compare it with transurethral resection of prostate (TURP). Methods   58 cases of BPH were randomly treated with 160W straight light beam greenlight PVP(n=28), or treated with TURP (n=30). All patients were followed up with operative time, hemorrhage volume during surgery, bladder irrigation time, catheter indwelling time, hospital stay time, residual urine volume(RUV), international prostatic symptom score(IPSS), quality of life(QOL), maxmium flow rates(Qmax), and operative complications. Results   All operations were successfully completed, and no severe complicaton occured. A significant difference in favor of straight light beam greenlight PVP was achieved for the bladder irrigation time, catheter indwelling time, hospital stay time and hemorrhage volume during surgery(P<0.05). A slight but not significant difference was found in operating time (P>0.05). The IPSS, QOL, Qmax and RUV were significantly improved in both groups after operations(P<0.05), but no significant differrence in the improvement of the subjective symptom and objective signs were found between the 2 groups(P>0.05). A 6-months followed-up indicates postoperative complications between the 2 groups were similar. Conclusion   The results of our study show that 160W straight light beam greenlight PVP for the treatment of BPH has the advantage of fewer blood loss, safer surgical procedures and shorter hospital stay time.It may be an ideal operation method for the treatment of BPH.

    In situ great saphenous vein bypass for the treatment of 
    Ⅲ、 Ⅳ arteriosclerosis obliterans of lower limb
    LI Yu-bin1, SUN Yan-yan1, JIANG Tong-bai1, SUN Da-jun2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  92. 
    Abstract ( 817 )   PDF (946KB) ( 390 )   Save
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    Objective   To evaluate the result of in situ great saphenouse vein bypass for the treatment of Fontaine Ⅲ、 Ⅳ arteriosclerosis obliterans of lower limb. Methods   26 patients with Fontaine Ⅲ、 Ⅳ arteriosclerosis obliterans of lower limb were treated with in situ great saphenouse vein bypass by using THE BUSHTM valvulotome.  Results   All cases of postoperative symptoms were alleviated. The rest pain disappeared, skin temperature significantly increased, ulcer healed with clear boundaries between the necrosis and the normal tissue in 25 cases. The rest 1 case was amputated in other hospital because of the postoperative thrombogenesis in the great saphenouse vein.The average ankle/brachial index (ABI) increased from 0.32 to 0.78. The cumulative patency rate at 6, 12 and 24 months was 96.1%, 88.5%, 80.8%, respectively. Conclusion   Application of in situ saphenous vein bypass according to the patients is an effective way to treat Fontaine Ⅲ、Ⅳ arteriosclerosis obliterans, especially those with severe symptoms of ischemia because of long-range, multi-segment, multi-planar stenosis or occlusion.

    The evaluation of the therapeutic effect of mixed bone autograft on
    Rand C proximal tibial defect in primary TKA
    HU Zhong-zhou, WANG Shao-jin, LIU Wen-guang, LIU Sheng-hou
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  96-99. 
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    Objective   To evaluate the therapeutic effect of mixed bone  autograft on Rand C proximal tibial defect in primary total knee arthroplasty (TKA). Methods   We retrospectively analyzed the data of patients from Feb. 2002 to Jun. 2007. 12 cases (male /female: 3/9), totally 16 knees with Rand C proximal tibial bone defect were handled with granular bone autograft combining with structural bone autograft by the same surgeon. The age of the subjects ranged from 50 to 79 (average 63.4). 11 cases (14 knees) suffered from osteoarthritis, and 1 case (2 knees) suffered from rheumatoid arthritis. The follow-up periods ranged from 12 to 48 months, averaged 26.8 months. Preoperative and postoperative X-ray and HSS score 3 days, 3 months, 12 months before and after surgery, and the latest follow-up were recorded respectively. Results   HSS score was promoted from preoperatively 22.5 (19 to 31) to 87 (78 to 96) points in latest follow-up, which has significantly difference (P<0.05), with 4 cases (6 knees) falling into excellent (≥90 points), 7 cases (9 knees) filling into good (80-89), and 1 case(1 knees) moderate (70-79). The excellent and good proportion could reach 93.75%. According to the Xray follow-up, no case was found failed to heal, and no grafts mobilization, bone absorption, bone dissolution, tibial plateau collapse and infection was detected. Although 2 knees were detected out with translucid bands(<1mm), loosening was negative. Conclusion   Autologous combined bone grafting is an effective procedure for treating Rand C proximal tibial bone defect in primary TKA, which could provide primary stability and result in good outcome. 

    Autogenous bone grafting in total hip arthroplasty for
    congenital acetabular dysplasia
    ZHOU Chui-bao, LI Ming, ZHANG Yuan-kai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  100-102. 
    Abstract ( 870 )   PDF (1113KB) ( 366 )   Save
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    Objective   To discuss the effect of the femoral-head autograft in uncemented total hip arthroplasty for congenital acetabular dysplasia. Methods   21 patients(24 hips) with acetabular dysplasia (Crowe type Ⅱ-or Ⅲ) from August 2007 to May 2010 received femoral head autograft in cementless total hip arthroplasty for acetabular dysplasia to reconstruct acetabula. Classified Harris Hip Score (CHHS) and imaging examination were used in the follow-up visit. Results   There was a significant difference between the pre and post operation according to CHHS (P<0.05). At an average follow-up of 27 months,all the patients had excellent clinical results. Neither acetabular autograft resorption nor non-union was observed. Conclusion   Using femoral-head autograft was an effect method in uncemented total hip arthroplasty for the acetabular dysplasia.

    Anatomical features and free transplantation of the
    posterolateral thigh perforator flap
    LIANG Xiao-qin1, GUO Yong-qiang2, WANG Jian-li2, HUO Ran1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  103. 
    Abstract ( 918 )   PDF (2487KB) ( 332 )   Save
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    Objective    To investigate the anatomic features of the posterolateral thigh perforator and the clinical application of the free posterolateral thigh perforator flaps for repairing the soft tissue defect in extremities. Methods   Fresh lower limb specimens underwent an intraarterial injection of a lead oxide and red gelatin preparation. The integument of the thigh was dissected, and perforators of 0.5mm or more in the posterolateral thigh region were identified. Their origin, course, distributon, and  anastomosis were observed. The distribution features of skin and vessels in the posterolateral thigh region were documented by angiography and photography. Free posterolateral thigh perforator flaps were used in 6 patients with the soft tissue defects. The size of the defects ranged from 10cm×5cm to 16cm×7cm. Results   The perforating arteries from profunda femoris created cutaneous perforators in the posterolateral thigh. Their average external diameter was about 1.2±0.3mm. The average pedical length from the deep fascia was 6.1~14.3cm. Cutaneous perforators could be found on a line extending from the ischium to the lateral femoral condyle and constructed a vascular net aline the axis. All flaps survived after surgery with the donor sites healed by first intention. After following up for 6~12 months, the appearance and function of the flaps were all satisfactory with excellent texture. Conclusion   The free posterolateral thigh perforator flap is easy to perform with constant location and excellent texture. It can be a useful alternative to repair the soft tissue defect of the extremities.

    Preparation of paclitaxel-loaded mixed micelles and
    enhancement on oral absorption of paclitaxel
    CUI Yanan1, GE Jianjun2, LI Lingbing1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  107-112. 
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    Objective   To prepare paclitaxel-loaded mixed micelles using D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) and phosphatidylcholine (PC) and study the effect on oral absorption in rats. Methods   Paclitaxel-loaded mixed micelles were prepared by the film dispersion method. Zeta potential and the diameter distribution of TPGS/PC mixed micelles were measured using the laser size scattering determinator. Morphology of micelles was observed by the transmission electron microscope. Dialysis method was used to evaluate the release behavior of drug-loaded micelles in vitro. The absorption kinetics was obtained by the in situ perfusion method in rats. Plasma paclitaxel concentration was determined by HPLC and the pharmacokinetics in rats was studied. Results   Mixed micelles were almost spherical with an average diameter of 78.34 nm and the Zeta potential was -7.84 mV. Compared to the bulk drug, paclitaxel mixed micelles accelerated Ka, prolonged tmax and increased Cmax  and AUC0-∞(P<0.05).  Conclusion   TPGS/PC mixed micelles can improve the oral absorption of paclitaxel, and increase its oral bioavailability.

    Socio-psychological characteristics and suicidal behavior of
    suicide attempters who ingested pesticides
    LIAN Ying1, JIA Cun-xian1, QIU Hui-min2, ZHANG Ji-yu3, LIU Hui1, HAN Mei1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  113. 
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    Objective   To find out the socio-psychological characteristics and suicidal behavior of suicide attempters who ingested pesticides and provide some evidences for suicide prevention. Methods   Six counties from disease surveillance points of Shandong province, China were selected, and the data of 409 attempted suicides happening from October 1, 2009 to March 31, 2011 were obtained. All subjects were divided into two groups: 344 individuals who ingested pesticides, and 65 who used other methods. Questionnaires of socio-psychological characteristics and Beck suicide intent scale (SIS) were used to obtain information from subjects. Results   The average age of all the subjects was (43.9±13.3) years old, and 67.7% of them were female. 344 (84.1%) suicide attempters ingested pesticides, while 65 (15.9%) used other methods. The percent of females (65.7%) and the scores of trait anxiety inventory, center for epidemiologic studies depression scale, and Beck hopelessness scale were lower in the suicide attempters who ingested pesticides than those who used other methods. The suicide attempters who ingested pesticides had significantly lower isolation (39.0%) at the time of committing suicide. There was no significant difference in total score of suicide intent between the two groups (4.40±2.66 vs 4.97±2.57, t=1.58, P=0.120). Multiple logistic regression analysis showed that suicide attempters who ingested pesticides were more likely to be married and had less mental disorders and physical illnesses (P<0.05). Conclusion   There are significant differences in sociopsychological characteristics between suicide attempters who ingested pesticides and those using other methods. Suicide prevention should focus on regulating the pesticides management and strengthening education.

    SFTSV RNA detection in sera of patients suffering from fever with
    thrombocytopenia syndrome
    CHI Yuan-yuan1, ZHAI Shen-yong2, WEN Hong-ling1, CUI Feng2, SONG Yan-yan1,WANG Ling2,DU Jun2, CAO Hai-xia2, WANG Qian3, ZHANG Shou-feng3, Y
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  118-121. 
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    Objective   PCR primers for amplification of severe fever with thrombocytopenia syndrome virus(SFTSV) were optimized in order to improve the detection sensitivity and specificity in sera from patients suffering from severe fever with thrombocytopenia syndrome(SFTS). Methods   RT-PCR primers were designed from the sequences of L, M, and S segments of SFTSV genome by using Primer Premier 5.0 and Oligo 6.0 softwares (L1,M1,S1) or obtained from references(L2, M2, S2). 12 primers were divided into 6 groups to amplify these segments. SFTSV RNAs in sera from patients with SFTS and normal sera were amplified by PCR and sequenced. SFTSV RNA detection sensitivity and specificity between primers were compared. At the same time, the relationship between the sensitivity and serum acquisition time were also compared. Results   RTPCR results showed that SFTSV detection sensitivity was different between primers. S-segment was most frequently amplified with one pair of primer(S1), while L-segment was amplified occasionally and M segment was never amplified. Sensitivity for the detection of SFTSV RNA in sera was 64.3% when it was perfomed within 7 d, compared with 37.5% in 7~14d. The difference between them was not statistically significant[P>0.05(=0.377)]. No segment of SFTSV was amplified in normal sera. Specificity of all primers was 100%. Conclusion   S1 primer pair is the most sensitive one among all primer pairs derived from S, M- and L-segments of SFTSV for RT-PCR amplification of S-segment.

    Analysis on diagnostic delay of tuberculosis patients and
    its influencing factors
    ZHAO Xiu-xiu, XU Ling-zhong, GUO Zhen, YANG Ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  122-125. 
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    Objective   To investigate the situation of diagnostic delay of tuberculosis patients and to explore its influencing factors in order to provide policy bases for improving the diagnostic delay. Methods   819 cases of tuberculosis patients were sampled from six cities of Shandong Province randomly, and the data was collected by questionnaire, which was analyzed by influencing factors test. Results   For the 819 cases of tuberculosis patients who were surveyed, 173(21.12%) of them were diagnosed delayed, and 643(78.88%) were not delayed. First diagnosis mechanism was the main cause that had something to do with the diagnostic delay of tuberculosis patients(P<0.05). Conclusion   Comprehensive measures should be taken to reduce the proportion of diagnostic delay of tuberculosis patients.

    Antibody response and the influencing factors after revaccination with
    three doses of hepatitis B vaccines among lowresponse adults
    L Jing-jing1,2, YAN Bing-yu2, CHEN Shi-yu3, XU Ai-qiang2, LIU Jia-ye2, FENG Yi2,GONG Xiao-hong4, CUI Fu-qiang4, LIANG Xiao-feng4, ZHANG Li2,
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2012, 50(11):  126. 
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    Objective   To evaluate the antibody response and influencing factors after revaccination with 3 doses of 4 types of hepatitis B vaccines among low-response adults.  Methods   The adults aged from 18 to 49 years old who were low response to the primary hepatitis B vaccine were randomly divided into 4 groups and revaccinated with one of the following types of hepatitis B vaccines at 0-1-6 schedule respectively: 10μg hepatitis B vaccine made by recombinant deoxyribonucleic acid techniques in Saccharomyces Cerevisiae (HepB-SC), 20μg HepB-SC, 10μg hepatitis B vaccine made by recombinant deoxyribonucleic acid techniques in Hansenula Polymorpha (HepBHP) and 20μg hepatitis B vaccine made by Chinese hamster ovary cell (HepB-CHO).  Questionnaire survey was made and blood samples were drawn from each person one month after the third dose. Hepatitis B virus surface antigen (Anti-HBs) were detected by the CMIA method. Influencing factors on antibody response were compared and analyzed by the multiple regression analysis.  Results   The total rate of anti-HBs seroconversion was 78.25%. The rates of anti-HBs seroconversion were 70.18%, 78.79%, 77.34% and 84.62% in the 10μg HepB-SC group, the 10μg HepB-HP group, the 20μg HepB-SC group and the 20μg HepB-CHO group respectively and the difference was statistically significant(P=0.004). The total corresponding geometric mean concentration (GMC) of anti-HBs was 443.92mIU/mL. The GMCs of anti-HBs of 4 groups were 297.03mIU/mL, 435.21mIU/mL, 445.76mIU/mL and 604.64mIU/mL. The GMCs were also significantly different by the groups(P<0.001). The multivariate analysis showed that the antibody level and the vaccine type for the primary immunization, and the vaccine type for the revaccination were related to the antibody response after the revaccination.  Conclusion   A good antibody response could be achieved by revaccination with 3 doses of hepatitis B among the low-response adults. The antibody response of 20μg HepB-CHO is better than 10μg HepB-SC.