Abstract:

Objective   To investigate the influence of PDCD4 gene to the differentiation of T lymphocyte subsets. Methods   Detect the expression differences of CD8+T, CD4+T and its subsets such as Th1、Th2, Th17 and CD25+Foxp3+Treg in the splenocytes and lymph nodes of pdcd4-/- mice and C57BL/6 mice by flow cytometry. And analyse the ratio change of the lymphocyte subsets. Results   Compared with that of the C57BL/6 mice, the number of CD8+T cells decreased in the pdcd4-/- mice(P<0.05). CD4+T and Th1 cells also showed a descending tendency but had no statistic significance(P>0.05). The proportion of CD4+CD25+Foxp3+Treg significantly increased in the pdcd4-/- mice (P<0.05).  Conclusion   PDCD4 can influence the differentiation of partial T lymphocyte subsets, the low expression of CD8+T cells as well as high proportion of CD4+CD25+Foxp3+Treg in the pdcd4-/- mice suggest that PDCD4 gene may play a role in the immune regulation.However, PDCD4 gene has no significant influence on the differentiation of CD4+T and Th1、Th2 and Th17 cells.

Key words: PDCD4 gene; Splenocyte; Lymph node; CD4+T cells； CD8+T cells; Th1 cells; Th2 cells; Th17 cells; CD25+Foxp3+Treg