JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (11): 53-57.

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Effects of TMPyP4-PDT on human cervical cancer cells

DING Bai-juan, ZHANG You-zhong, L Chang-shuai, JIANG Kan, LIU Hong-li   

  1. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2012-03-20 Online:2012-11-10 Published:2012-11-10
  • Contact: 张友忠(1963- )，男，博士，教授，博士生导师，主要从事妇科肿瘤的研究。 E-mail: zhangyouzhong@vip.sina.com

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Abstract:

Objective   To investigate the effect of photodynamic therapy mediated by meso-5,10,15,20-Tetrakis(N-methyl4-pyridyl) porphine (TMPyP4) on human cervical cancer HeLa cells in vitro. Methods   The proliferative activity of HeLa cells was assessed by Cell Counting Kit-8 (CCK8) assay, and the modality of TMPyP4-PDT treated cells were observed with light microscope. The apoptosis was examined by Annexin-V-FITC/PI double dye kit, and the expressions of c-myc mRNA and hTERC mRNA of the cell lines were tested by RT-PCR. Results   At the certain range of the concentration, the inhibition rate and induced cell apoptosis rate mediated by TMPyP4-PDT increased with the increase of the dosage and the light energy. The necrosis and apoptosis cells were observed with light microscope. The expressions of c-myc and hTERC mRNA were decreased. Conclusion   TMPyP4-PDT has a distinctive killing effect on HeLa cells, which shows a concentration-dependent manner and an energy-dependent manner. The possible mechanism may be related with down-regulating the expressions of c-myc mRNA and hTERC mRNA.

Key words: Cervix neoplasms; HeLa cells; Meso-5,10,15,20-Tetrakis- (N-methyl-4-pyridyl) porphine; Photodynamic therapy； c-myc mRNA； Human telomerase gene mRNA

CLC Number: 

  • R737.31
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