Abstract:

Objective   To study the effects of duodenal jejunal bypass(DJB) on the expression of Glucagon-like peptide-1receptor (GLP-1R) in the enteric nervous system of type 2 diabetic(T2DM)rats, and to explore the relation-ship between GLP-1R expression， GLP-1 secretion and blood glucose. Methods   Wistar rats were randomly divided into four groups: Wistar control group (W-C)， Wistar DJB surgery group (W-DJB)， T2DM control group(T2DM-C)， T2DM DJB surgery group (T2DM-DJB)， 8 rats in each group. Fasting blood glucose and glucagon-like peptide-1 (GLP-1) were detected before DJB operation and 1week， 4 weeks， 8 weeks after DJB operation． Meanwhile, fasting insulin before DJB operation and 8 weeks after DJB operation was also detected. After 8 weeks, the rats were sacrificed and the expression of GLP-1R in the enteric nervous was studied by RT-PCR and immunofluorescence. Results   The number of GLP-1R positive cells in the T2DM-DJB rats enteric nervous system was 15.83±2.23， it increased significantly compared to that of the T2DM-C rats which was 8.50±2.67(P<0.01). The expression of GLP-1R mRNA in the T2DM-DJB rats enteric nervous system was 0.67±0.08, it also increased significantly compared to that of the T2DM-C rats which was 0.42±0.03(P<0.01). The fasting blood glucose and fasting insulin of T2DMDJB rats decreased after DJB surgery(P<0.01), and the fasting serum GLP-1 increased after DJB surgery (P<0.01). GLP-1R expression level, fasting blood glucose, fasting insulin and fasting serum GLP-1 of the W-DJB rats had no significant difference after DJB surgery compared to those of the W-C rats(P>0.05). Conclusion   DJB surgery can significantly decrease the fasting blood glucose of T2DM rats, but it has no obvious effect in normal rats.

Key words: Duodenal-jejunal bypass surgery； Diabetes mellitus, Type 2； Enteric nervous system; Glucagon-like peptide-1 receptor