JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (11): 43-47.

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TWEAK promotes expressions of collagen I and matrix metalloproteinase1
in rat cardiac fibroblasts via P38MAPK pathway

WANG Qi-lei1, REN Man-yi2, WANG De-jin1, XU Dong-ling1,
DU Yi-meng1, WANG Xu-ping3, SUI Shu-jian1   

  1. 1. Department of Cardiology， the Second Hospital of Shandong University, Jinan 250033,  China;
    2. Department of Cardiology, Shandong Provincial Chest Hospital, Jinan 250133, China;
    3． Department of Cardiology, Qilu Hospital of Shandong University, Key Laboratory of Cardiovascular Remodeling and
    Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Jinan 250012, China
  • Received:2012-03-29 Online:2012-11-10 Published:2012-11-10

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Abstract:

Objective   To investigate the mechanism and effects of tumor necrosis factor-like inducer of apoptosis (TWEAK) on expressions of collagen I and matrix metalloproteinase1(MMP-1) in cardiac fibroblasts(CFs). Methods   Cultured CFs from neonatal Wistar rats were treated with the recombinant human TWEAK and the P38MAPK specific inhibitor(SB203580). CFs were divided into 3 groups: ① the control group: CFs were cultured without any stimulus; ② the TWEAK group: CFs were incubated with 100ng/mL TWEAK; ③ the TWEAK+SB203580 group: CFs were incubated with 100ng/mL TWEAK+SB203580. The CFs proliferation was examined by MTT, and expressions of collagen Ⅰ-and phosphor-P38MAPK protein were detected by Western blot. qRT-PCR was used to detect the collagen Ⅰ mRNA level. ELASA was used to detect the concentration of MMP1 in CFs cell-culture supernatant. Results   TWEAK promoted the proliferation of the CFs and expressions of collagen Ⅰ and phosphor-P38MAPK protein. The expressions of collagen Ⅰ mRNA and MCP-1 were up regulated due to TWEAK. P38MAPK specific inhibitor (SB203580) could inhibit the proliferation of CFs. P38MAPK specific inhibitor (SB203580) could partially inhibit expressions of collagens Ⅰ mRNA , collagens Ⅰ protein, phosphor-P38MAPK protein and MMP-1. Conclusion   TWEAK can promote expressions of collagen Ⅰ and MMP-1 in rat cardiac fibroblasts via P38MAPK pathway.

Key words: TWEAK; Cardiac fibroblasts; P38MAPK; Collagen I; MMP-1

CLC Number: 

  • R542.2
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