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Table of Content

      
    16 February 2009
    Volume 47 Issue 02
    无栏目
    Construction and expression of eukaryotic expression
    plasmid of microRNA let7a2 in lung cancer cells
    GUAN Hengyun1, ZHANG Ju1, ZHANG Pengju1, CHEN Weiwen1,　 LIU Wenwen2, YU Chunxiao3, HU Xiaoyan1, JIANG Anli1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  1-5. 
    Abstract ( 1047 )   PDF (755KB) ( 315 )   Save
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    To construct a recombinant eukaryotic expression plasmid of microRNA let7a2 and express it in lung cancer A549 cells. Methodslet7a2 precursor sequence was amplified by RTPCR using RNA from A549 cells and was cloned into the pSilencer4.1 CMV neoexpression vector to produce the recombinant pSilencer4.1let7a2. Then the recombinant pSilencer4.1let7a2 was transfected into lung cancer A549 cells, and the prelet7a2 expression was verified by RTPCR. According to the miRBase Targets database, the target sequence of let7a2 was synthesized and inserted to the pMIRReport luciferase reporter vector to construct pMIRReportlet7a2T, which was cotransfected with pSilencer4.1let7a2 into A549 cells, and the relative luciferase activity was detected. The effect of let7a2 on A549 cell proliferation was tested by MTT. ResultsThe results of DNA sequencing showed that sequences of pMIRReportlet7a2T and pSilencer5.1let7a2 were correct. RTPCR prelet7a2 was overexpressed in A549 cells after transfection of pSilencer4.1let7a2. Cotransfection of pSilencer4.1let7a2 and pMIRReportlet7a2T showed that mature let7a2 had biological activity. MTT results indicated that let7a2 inhibited proliferation of the transfected A549 cells. ConclusionThe eukaryotic expression plasmid of let7a2 was successfully constructed and effectively expressed in A549 cells.

    Human cytomegalovirus infection upregulates expression of LOX1 in
    rabbit smooth vascular muscle cells
    LEI Xueming1,　LI Shuying1,　DONG Bo2,　PENG Ying1,　TANG Wei1, QI Mei1,LIU Juan1,　SUN Yundong1,　YU Han1,　ZHOU Yabin1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  6-9. 
    Abstract ( 1042 )   PDF (584KB) ( 278 )   Save
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    To investigate the effect of human cytomegalovirus(HCMV) infection on expression of lectinlike oxidized lowdensity lipoprotein receptor1 in rabbit smooth vascular muscle cells, and to show its role in the formation of atherosclerosis (AS). MethodsExpression of LOX1 in rabbit smooth vascular muscle cells which were affected by HCMV ADl69 was analyzed by reverse transcriptionpolymerase chain reaction and Western blotting. ResultsHCMV infection upregulated the expression of LOX1 in rabbit smooth vascular muscle cells. ConclusionHCMV infection upregulates the expression of LOX1 and this may be one of the mechanisms by which Human cytomegalovirus is involved in atherosclerosis．
    Immunotherapeutic effect of dendritic cell vaccine modified with the CXCL10
    gene and tumor cell RNA on mice with prostate cancer
    LI Bo1, ZHAO Qingli1, LUN Xiaoqin2, WEI Xuebin1, LI Shun1, LI Qing1

    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  10-13. 
    Abstract ( 991 )   PDF (285KB) ( 350 )   Save
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    To evaluate the effect of therapeutic vaccine against prostate cancer based on dendritic cell (DC) vaccine modified with tumor cell RNA and the CXCL10 gene both in vitro and in vivo. MethodsMouse bone marrow DC was pulsed with cell RNA from the RM1 prostate cancer cell line and then transfected with the recombinant plasmid CXCL10／pcDNA31 by DOTAP liposome. The immunotherapeutic effects of DC vaccine on mice with prostate cancer were assessed. ResultsThe DC vaccine increased the preferential chemotaxis to T cells, and the mice immunized with DC vaccine exhibited a specific CTL response compared with other groups. In the C57BL／6 mice model with preestablished subcutaneous RM1 prostate cancer, immunization of DC vaccine inhibited tumor growth more significantly when compared with other counterparts and the survival time of micetreated with the DC vaccine was greatly extended. The DC vaccine immunized mice also exhibited an effective resistance to tumor challenge. ConclusionDC vaccine modified with CXCL10 gene and tumor cell RNA might elicit significant antitumor effects through efficient induction of antitumor immunity and might be of therapeutic potentials for prostate cancer.
    Effects of Huayulifei on the expressions of MMP2 and TIMP1 in
    rats with bleomycininduced pulmonary fibrosis
    WANG Qin1, LIU Shiqing2, LI Changsheng3, LU Wei4
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  14-17. 
    Abstract ( 1081 )   PDF (709KB) ( 267 )   Save
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    To evaluate the effects and mechanism of Huayulifei on blemycininduced pulmonary interstitial fibrosis in rats. MethodsRats were randomly divided into the control group, the model group, the Huayulifei group and the hydrocortisone group. Rat pulmonary fibrosis was induced by blemycin(5?mg/kg) in the experimental groups and 0.9% saline in the control group. Rats were killed on the 7th，14th and 28th days. Expressions of MMP2 and TIMP1 proteins were determined by an immunohistochemical technique. Results① The airsacculitis and fibrosis of lungs in the model group were fairly severe which had much deposition of collagen fibers. In the Huayulifei group which had few inflammatory cells, the alveoli structure was nearly normal, and most of the airsacculitis and fibrosis of lungs were mild. In the Hydrocortisone group, alleviation of airsacculitis and fibrosis of lungs were similar with those of the Huayulifei group. ② In the Huayulifei group, expressions of MMP2 protein and TIMP1 protein were significantly lighter than those in the model group. ConclusionHuayulifei can ameliorate blemycininduced pulmonary fibrosis in rats, and it may partly work by affecting the expression of MMP2/TIMP1.
    Effect of ketamine on cerebral HSP70 expression in
    rats after severe burns
    XU Yanbing, DONG Ling, ZHANG Mengyuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  18-20. 
    Abstract ( 931 )   PDF (472KB) ( 228 )   Save
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    To investigate the effect of ketamine on cerebral HSP70 expression in rats after severe burns and to explore its possible mechanism of cerebral protection. MethodsOne hundred and twentyfour male Wistar rats were randomly divided into 3 groups: the normal control group(group N) (n=20)，　the burned control group (group B)(n=52) and the ketamine group (group K) (n=52). Rats in groups B and K were respectively sacrificed at 3, 6, 12 and 24?h after administration. There were 8 rats for each time point of the two groups. Myocardium samples were collected from all groups for HSP70 detection by Western blot analysis. Survival rates of the other rats of each group were measured at 3?h, 6?h, 12?h, 24?h, 2?d, 4?d, 8?d and 10?d. ResultsHSP70 expression was higher in groups K and B than in group N at 3, 6, 12 and 24?h(P＜0.05). It reached a peak at 12?h after administration. HSP70 expression was higher in group K than in group B at 3 and 6?h(P＜0.05). The survival rate of group K was higher than that of group B(P＜0.05). ConclusionsKetamine can enhance cerebral HSP 70 expression in rats after severe burns.
    Pretreatment with lowdose lipopolysaccharide protects against acute liver injury induced by lipopolysaccharide in mice: A study of mechanism
    GENG Jiawei1, ZHOU Wei2, FAN Hong1, HUANG Youguang3，　ZOU Chenggang2, LI Shude3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  21-24. 
    Abstract ( 1034 )   PDF (629KB) ( 301 )   Save
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    To explore the effect of lowdose lipopolysaccharide (LPS) on acute liver injury induced by highdose lipopolysaccharide in mice. MethodsThe mouse model of acute liver injury was reproduced by intraperitoneal administration of LPS. GSH in the livers of mice was depleted by administering buthionine sulphoximine (BSO). Aspartate transaminase (AST) and alanine transaminase (ALT) in the plasma were measured by an automated biochemistry analyzer. The mRNA levels of cystathione (synthase (CBS) and γglutamylcysteine synthetase (GCS) were determined by RTPCR. Results① Pretreatment with lowdose LPS attenuated acute liver injury induced by highdose LPS in mice. ② Lowdose LPS treatment elicited hepatic glutathione (GSH) levels in mice. The levels of GSH in the group pretreated with lowdose LPS were higher than those in the group treated with highdose LPS. ③ Plasma levels of ALT and AST in the group pretreated with lowdose LPS were similar to those in the group treated with highdose LPS. ④ Lowdose LPS pretreatment resulted in an increase in CBS activity as well as GSC mRNA levels. ConclusionLowdose LPS attenuates acute liver injury induced by highdose LPS in mice by promoting hepatic GSH levels though upregulating activity or expression of GSH biosynthesisrelative genes.
    Construction of fluorescent proteinexpression of the human cytomegalovirus and determination of its action
    PENG Ying， ZHANG Xiaomei， LEI Xueming， CHENG Yizhe, SUN Yundong, LIU Juan, YU Han, QI Mei， WANG Xiaoyan，ZHOU Yabin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  25-29. 
    Abstract ( 999 )   PDF (675KB) ( 122 )   Save
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    ObjectiveTo construct fluorescent proteinexpressing human cytomegalovirus （HCMVTowneGFP）and to study its application in screening antiviral agents. MethodsThe plasmid PHM673 was amplified and purified, and then was transfected into primary culture HELF via LipofectamineTM 2000. Then GFPHCMV was purified by plaque assays and the presence of the recombinant virus was confirmed by PCR. The antiviral agent ganciclovir was screened using the GFPbased antiviral assay. ResultsGFPHCMV was successfully constructed and there was no significant difference in activity between the recombinant virus and original virus. The growth and reproduction of recombinant virus were not affected. Fluorescence expression of the experimental group was related to the virusinhibitory effects of the specific antiviral agent ganciclovir. ConclusionGFPHCMV may be a new tool to research the pathogenic mechanism and screen antiviral agents.
    Evaluation of dual source CT angiography in diagnosis of
    etiopathogenisis in vertebrobasilar artery insufficiency
    LONG Na, LIU Yaqun, LI Dumin, CUI Fengyu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  30-33. 
    Abstract ( 903 )   PDF (495KB) ( 130 )   Save
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    To investigate the diagnostic value of dual source CT angiography (DSCTA) on etiopathogenisis of vertebrobasilar artery insufficiency (VBI) by utilizing DSCTA to display pathological changes of vertebrobasilar arteries. Methods43 patients suspected of vertebrobasilar artery insufficiency（VBI）were subjected to DSCTA. Then the original data were transported to the workstation MMWP8818 for restitution postprocessing. The vertebrobasilar arteries were evaluated by correlated grading rules. ResultsOf the 86 vertebral arteries in 43 patients, 12 arteries in 6 patients were normal. Of the 74 arteries in 37 patients, 27 arteries had stenosis, in which 21 arteries were caused by scleratheroma plaque and 6 arteries by cervical vertebrae oppression, 18 arteries had circuity, 18 arteries had inborn variation, and 1 artery had an aneurysm. Some of the arteries had more than two kinds of pathological changes. By DSCTA, 37 of 43 patients had etiopathogenisis (86%). In a contrast analysis between DSCTA and DSA, the coincidence rate of vertebral stenosis with DSCTA in sensitivity and specificity was 84.6% and 94.7%. ConclusionDSCT angiography has conspicuous superiority in displaying the pathological changes of vertebrobasilar arteries by various kinds of postprocessing skills, and DSCT angiography may become a conventional imaging examination for VBI.
    Dual source CT in detection of the sinus node artery
    and its clinical significance
    YU Dexin, WEI Huagang, WANG Qian, WANG Qing, CUI Fengyu, MA Xiangxing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  34-37. 
    Abstract ( 990 )   PDF (451KB) ( 227 )   Save
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    ObjectiveTo detect the sinus node artery (SNA) in adults with dual source CT. MethodsTwo hundred and seven cases that had undergone dual source CT coronary artery angiography were included in this study. The visualized grades of SNA were accessed by two radiologists based on the length and delineation of SNA on CT images. The diameter of SNA and the length from the origin of SNA to the origin of the coronary artery were measured. The relationship of calcification on the coronary artery wall around the origin of SNA with the diameter and the visualized grade of SNA were also analyzed. ResultsThe detected ratio of left, right and bilateral types of SNA was 64.3%, 20.3% and 15.5 % respectively in a total of 239 SNA, and the best visualization of left and right SNA was 77.0% and 81.2%. The normal SNA diameter was (0.20±0.04)mm, however it was (0.18±0.03)mm when calcification occurred on the coronary artery wall around the origin of SNA(t＝2.558, P=0.011). In addition, calcification also affected the observation of SNA such as length and delineation (P＜0.05). ConclusionA good way to detect SNA is by using dual source CT, and calcification on the coronary artery may affect the blood supply of SNA.
    Association analysis of the IL12B gene polymorphisms with chronic obstructive pulmonary disease in the Chinese Han population
    YIN Yingqiu1, WANG Aihua1, KONG Lingmin2, ZHANG Min3, MA Daoxin4, YU Qinfeng1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  38-41. 
    Abstract ( 983 )   PDF (322KB) ( 288 )   Save
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    To explore the relationship between single nuclear polymorphism (SNP) of 1188A/C in the interleukin 12B gene and susceptibility of chronic obstructive pulmonary disease (COPD) in the Chinese Han population from Shandong Province. MethodsSNP 1188A/C of the IL12B gene was detected by polymerase chain reaction (PCR) based on the restriction fragment length polymorphism (RFLP) technique in 324 COPD subjects and 389 healthy controls. ResultsThe frequencies of genotypes AA, AC, and CC at polymorphism sites 1188A/C were 0.333?3, 0.478?4, and 0.188?3 respectively in the COPD group, while they were 0.357?3, 0.475?6, and 0.167?1 in the control group. Also, the A type allele frequencies were 0.572?5 and 0.595?1 in patients and controls, and the C type allele frequency was 0.427?5 and 0.404?9 in COPD patients and healthy controls. There were no significant differences in genotype frequencies (P value=0.689) or allele frequencies between the two groups (P=0.389). ConclusionNo significant association was found between IL12B 1188A/C polymorphism and COPD susceptibility in the Chinese Han population from Shandong Province.
    Threedimensional conformal radiotherapy with weekly paclitaxel and
    carboplatin for local advanced nonsmall cell lung cancer
    HAO Chengcheng, LIU Huizhong, WANG Junchao, WANG Xianglian, TIAN Guangliang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  42-45. 
    Abstract ( 1083 )   PDF (297KB) ( 145 )   Save
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    To evaluate the efficacy and toxicity of threedimensional conformal radiotherapy with weekly paclitaxel and carboplatin for local advanced nonsmall cell lung cancer (NSCLC). Methods94 patients with local advanced NSCLC were randomly divided into three groups: the concurrent chemoradiation group A(n=33), the concurrent chemoradiation group B(n=29), and the sequential chemoradiation group(n=32). The three groups were all given 3DCRT at 2?Gy/f to a total dose of 70?Gy. In the concurrent chemoradiation group A, paclitaxel 50?mg/m2 and carboplatin of 1.5AUC/cycle were given on day 1, 8, 15, 29, 36 and 43. In the concurrent chemoradiation group B, except for paclitaxel 40?mg/m2, paclitaxel 50?mg/m2 and carboplatin of 1.5AUC/cycle were also given on the above mentioned days. In the sequential chemoradiation group, two chemotherapy cycles were given before radiotherapy. Paclitaxel 150?mg/m2 and carboplatin 5 AUC/cycle were administered on day 1 and 21. ResultsThe effective power 〔complete response(CR) +partial response(PR)) of concurrent chemoradiation groupA, B, and sequential chemoradiation group was 81.8%, 79.3%, and 56.2%. The median survival time was 18.8 months for the concurrent chemoradiation group A, 17.6 months for the concurrent chemoradiation group B and 15.6 months for the sequential chemoradiation group. The 1 and 2year survival rates in the three groups were 74.0% and 41.0%, 72.0% and 39.0%, and 69.0% and 35.0%, respectively. ⅡⅢ grade of esophagitis, pneumonia and leukocytonpenia in the three groups were 60.6%, 24.2%, and 42.4%; 58.6%, 24.1% and 44.8%; and 40.6%, 21.9% and 18.8%, respectively. Conclusion
    Expression of villin in precancerous lesions and gastric
    carcinoma and its clinical significance
    ZHAO Chengbo1, ZHOU Chengjun2, GUO Jianqiang2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  46-49. 
    Abstract ( 1042 )   PDF (488KB) ( 271 )   Save
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    To explore expression of villin in normal gastric mucosa tissues, intestinal metaplasia(IM), dysplasia, and gastric carcinoma and its clinical significance. MethodsAn immunohistochemical method was used to determine villin expression. ResultsVillin was expressed in dysplasia cells but not in gastric mucosa. The positive rates of its expression in intestinal metaplasia, dysplasia, and gastric carcinoma were 85.0%(17/20), 66.7% (20/30), and 48.3%(29/60), respectively. It was also found that expression of villin had no relationship to tumor size, invasiveness, and clinical stage and metastasis to lymphnodes(P>0.05), however, expression of villin was significantly higher in well moderately differentiated cancers than in poorly differentiated ones (P<0.05). ConclusionExpression of villin in precancerous lesions and gastric carcinoma is downregulated and is closely related to tumor differentiation degrees. Villin is a good marker for screening different types of gastric carcinoma and has a certain guiding sense to early diagnosis and clinical treatment.
    Apolipoprotein E gene expression in peripheral blood monocyte and lipid analysis of hypertension patients
    LI Jianzhong, ZHAO Jun, GUO Guangqing， CHEN Qi, LIU Jinxia, ZHANG Yanguo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  50-52. 
    Abstract ( 897 )   PDF (307KB) ( 268 )   Save
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    To explore the expression of the apolipoprotein E (Apo E) gene in peripheral blood monocytes and its relationship with lipids and hypertension. MethodPeripheral blood monocyte were isolated and Apo E gene expression was determined by the RTPCR method, and also the lipid level was determined. ResultsThe Apo E gene was expressed in peripheral blood monocytes, and it was lowerexpressed in the high risk group and very high risk group（P＜0.05）. The higher the risk grade, the lower the Apo E gene expression. The LDLC level was negatively correlated with the Apo E gene(r=-0.979, P＜0.05), whereas it was not correlated with TC, TG, apoB100, apoA and Lp(α) （P＞0.05）. ConclusionExpression of the Apo E gene in peripheral blood monocytes of the high risk group and very high risk group is significantly decreased and is related to abnormal lipid metabolism, which suggests that changes of expression of the Apo E gene is related to the development of hypertension.
    Immunophenotyping in myelodysplastic syndrome and aplastic anemia
    ZHAO Guangling1, XU Hongzhi1, HUANG Min1, LI Yuantang2,
    WU Huanling2, LI Jianfeng3, MA Chunyan3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  53-57. 
    Abstract ( 1113 )   PDF (277KB) ( 296 )   Save
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    To explore the role of immunophenotyping in the pathogenesis, diagnosis, classification and differential diagnosis of myelodysplastic syndrome (MDS) and aplastic anemia(AA). MethodsA panel of monoclonal antibodies and flow cytometry using CD45/SSC gating strategies were used for immunophenotyping in bone marrow nucleated cells of 23 patients with MDS, 14 patients with AA and 9 healthy controls. ResultsCompared with the control group, expressions of haemopoietic stem/progenitor cell surface markers CD34 and HLADR, early medullary system surface markers CD13 and CD33, monocyte system surface marker CD14, and T lymphocyte cell surface marker CD7 were significantly increased in the MDS group; however, expressions of later medullary system surface marker CD15, B lymphocyte cell surface markers CD19 and CD20 were significantly decreased in the MDS group（P＜0.05）. However, as RA progressed to RAEB, expressions of CD34, HLADR, CD13, CD33, CD14 and CD7 were significantly increased, while expressions of CD15, CD3, CD19 and CD20 were significantly decreased; and the DI level was significantly increased（P＜0.05）. Expression levels of CD34, CD13, CD33 and CD15 in the AA group were significantly higher than those in the control group and those of CD3, CD7, CD25 and CD22 were lower than those in the control group（P＜0.05）. Also expression levels of CD34, HLADR, CD13, CD33 and CD14 in the MDS group were significantly higher than those in the AA group, while expression levels of CD3, CD5, CD7, CD15, CD19, CD20, CD22 and CD25 were significantly lower in the MDS group than those in the AA group（P＜0.05）. ConclusionImmunophenotyping analysis may be a useful tool for the diagnosis, classification and differential diagnosis of MDS and AA.
    Expression of peripheral blood lymphocyte ubiquitin mRNA in chronic HBV infection with active inflammation and its clinical significance
    WANG Xiaoju1，　WANG Mingming2，　XU Wansu2,　ZHAO Shengmei3，CUI Sunan2，　LI Xiaoying2，　LIU Chunhua1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  58-61. 
    Abstract ( 978 )   PDF (662KB) ( 311 )   Save
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    To investigate the expression of peripheral blood lymphocyte ubiquitin mRNA in chronic HBV active infection and to evaluate its clinical significance. MethodsNinetysix patients were enrolled in the study and were divided into 3 groups： the chronic hepatitis B group（40 patients）, the active cirrhosis group(30 patients) and the severe hepatitis group（26 patients）. In addition, 26 healthy persons served as the control group. Ubiquitin mRNA of the peripheral blood lymphocytes and intrareference βactin were determined by realtime PCR. Differences among groups were analyzed by oneway ANOVA. ResultsExpression level of peripheral blood lymphocyte ubiquitin mRNA in all experimental groups was lower than in the healthy control group(P=0.00), and in the severe hepatitis group it was the lowest and in the active cirrhosis group it was the second. ConclusionExpression of the peripheral blood lymphocyte ubiquitin mRNA is abnormal in all patients, and it may be involved in the occurrence and development of chronic HBV active infection. The lower the expression, the severer the disease.
    11391G/A polymorphism in the 5′ promoter region of the APN gene contributes to the development of type 2 diabetes
    ZHAO Haiyan1, SUN Wei2, ZHANG Xia2, ZHANG Ping2, SU Benli2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  62-64. 
    Abstract ( 1053 )   PDF (254KB) ( 263 )   Save
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    To investigate whether the APN gene 11391G/A and 11377C/G polymorphism are associated with type 2 diabetes mellitus in Chinese people. MethodsA total of 258 Dalian subjects in China were selected. 172 had type 2 diabetes and 86 did not have type 2 diabetes. Genotyping of APN was undertaken with the PCRRFLP method. ResultsThe 11377C/G polymorphism was not associated with type 2 diabetes and 11391G/A was related to type 2 diabetes (χ2=7.662, P<0.005). ConclusionThe presence of the11391A allele might lead to compromised beta cell function in type 2 diabetes.
    Genetic polymorphism of thrombinactivatable fibrinolysis inhibitor (TAFI)
    associated with type 2 diabetes mellitus
    WU Xiaoben1，　XU Chengwei1，　SUN Fudun2,　ZHAO Jingjie3，　WANG Lili1, WANG Zejun1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  65-69. 
    Abstract ( 999 )   PDF (770KB) ( 233 )   Save
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    To investigate the possible association of the TAFI activityrelated two polymorphisms (505A/G, 1040C/T) with type 2 diabetes mellitus (T2DM). MethodsFrequencies of the 505A/G and 1?040C/T polymorphisms resulted in two amino acid substitutions: Ala for Thr at position 147 and Ile for Thr at position 325 in 157 T2DM patients and 140 healthy controls by using the polymerase chain reactionrestriction fragment length polymorphism(PCRRFLP) typing analysis method. ResultsNo significant differences of the TAFI levels appeared between patients and controls at 505A/G. At 1040C/T, frequency for the T allele in the patient group was significantly lower than that in the control group (15.6% versus 25.7%, P＜0.05), which was due to the relative decrease of T/T homozygotes in the patient group (P＜0.05, 95% OR 0.28,CI 0.110.70). The same pattern of significance was also observed between controls and subgroups of patients with initial stages of T2DM categorized by the urine albumin excretion (UAE μg/mL)tocreatinine (mg/mL) ratios (ACRs). Conclusion① At 1?040C/T the frequency for the T allele is strongly associated with T2DM in a subset of the general population. ② The T allele confers protection against the onset of T2DM only in homozygosity and may serve as a recessive trait.
    Effects of GnRHa and Gestrinone on growth and apoptosis
    in ectopic endometrial stroma cells
    WANG Chunfeng, JIANG Weiguo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  70-73. 
    Abstract ( 894 )   PDF (640KB) ( 242 )   Save
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    ObjectiveTo investigate the effects of GnRHa and Gestrinone on growth, proliferation and apoptosis in isolated ectopic endometrial stroma cells in vitro, as well as expression of MMP3 induced by GnRHa and Gestrinone. MethodsAxenic ectopic endometriotic tissues were isolated and cultured in vitro. Then the cultured endometrial stroma cells were observed by an inverted microscope and identified by vimentin immunohistochemical staining. They were cultured and exposed to GnRHa and Gestrinone at different doses. The inhibition of the cells in 60 hours was determined by methylthiazolyl tetrazolium (MTT) assay, and a cell growth curve was made. At 48 hours the cell cycle and apoptosis were monitored by flow cytometry (FCM), and expression of MMP3 in isolated ectopic endometrial stroma cells was analyzed by immunohistochemistry. ResultsGnRHa and Gestrinone obviously blocked the cell cycle of ectopic endometrial stroma cells with the number of cells increased at the G1 stage and decreased at the S stage. The apoptotic rate of endometrial stroma cells was significantly increased (P<0.05), while expression of MMP3 induced by GnRHa and Gestrinone was obviously decreased (P<0.05), but the apoptosis was more significantly enhanced and expression of MMP3 was more obviously decreased by GnRHa than by Gestrinone. ConclusionsGnRHa and Gestrinone can significantly inhibi growth and induce apoptosis of ectopic endometrial stroma cells by downregulating MMP3 expression. GnRHa is more effective than Gestrinone.　　　　　 　　　　　　　　　　　　　
    Expressions of B7H4, CA125 and P53 in epithelial
    ovarian cancer and their clinical value
    KONG Fanrong1,　WANG Liming1,　DAI Shuzhen1,　SUN Xianlu2,　L Yanhua1

    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  74-77. 
    Abstract ( 1017 )   PDF (459KB) ( 327 )   Save
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    To investigate expressions of B7H4, CA125 and P53 in primary epithelial ovarian cancer (EOC) and to explore their relationship with prognosis. MethodsExpressions of B7H4, CA125 and P53 were determined by PV6000 twostep immunohistochemistry in 70 cases of EOC, 20 cases of normal ovarian tissues and 20 cases of benign ovarian tumors. ResultsThe positive rate of B7H4 in epithelial ovarian cancer was 88.5%(62/70), and it was significantly higher than that in benign ovarian epithelial tumors (45%) and normal ovarian tissues (0%) .The positive rate of CA125 in epithelial ovarian cancer was 77.1%, and it positively correlated with B7H4. The positive rate of P53 in epithelial ovarian cancer was 68.6% (48/70), and was significantly lower than B7H4 (P<0.05). Multivariate COX regression analysis showed that prognosis of epithelial ovarian cancer was correlated with the FIGO stage, residual tumor size, B7H4′s positive expression, cell differentiation, CA125′s positive expression, P53′s positive expression and lymph node metastasis. Single factor KaplanMeier analysis showed that the intensity the B7H4 positive expression was negatively correlated with the average survival time of patients and the cumulative fiveyear survival rates. ConclusionB7H4 overexpresses in epithelial ovarian cancer and positively correlates with CA125 and is higher than P53 expression. B7H4 is an independent risk factor of total life span of epithelial ovarian cancer patients. The stronger the B7H4 expression, the shorter the average life time and the lower the cumulative fiveyear survival rate.
    Clinical significance of lymphatic vessels density marked by LYVE1
    in the earlystage of invasive carcinoma of cervical uteri
    YU Hao, ZHANG Linlin, ZHANG Shiqian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  78-81. 
    Abstract ( 1027 )   PDF (464KB) ( 271 )   Save
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    To investigate the expression and its clinical significance of lymphatic vessels density (LVD) stained by lymphatic vessel endothelial hyaluronan receptor1（LYVE1）in the earlystage of invasive carcinoma of cervical uteri. MethodsImmunohistochemical staining with LYVE1 was used to determine LVD in 41 cases of earlystage of invasive carcinoma of cervical uteri and 12 cases of normal cervical uteri, and their correlation with tumor clinicopathological features was analyzed. Results①The majority of peritumoral lymphatic vessels was often enlarged and irregular. In contrast, the intratumoral lymphatic vessels were small and collapsed. The peritumoral lymphatic vessels density (PLVD) was significantly higher than the intratumoral lymphatic vessels density (ILVD)(P＜0.01). Compared with the LVD of benign cervix, both ILVD and PLVD were more significantly increased (P＜0.01).②Both ILVD and PLVD in patients with lymph nodal metastasis were significantly higher than those without lymph nodal metastasis (P＜0.05, P＜0.01). The same happened in the relationship between lymphatic vessel invasion and ILVD or PLVD (P＜0.05, P＜0.01). Both ILVD and PLVD in patients with histological grade G2 and G3 were more significantly increased than G1 (P＜0.05), but there were no differences between G2 and G3 (P＞0.05). ConclusionCompared with the LVD of benign cervix, both ILVD and PLVD are significantly increased and correlated with lymph node metastasis and lymphatic vessel invasion. Tumor lymphangiogenesis in the earlystage of invasive carcinoma of cervical uteri may play an important role in the process of lymphatic metastasis.
    Kidneybenefiting and conceptionaiding granules in treating infertility due to ovulatory dysfunction
    CHANG Fuli，LI Shuling ， LI Yuzhu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  82-84. 
    Abstract ( 1085 )   PDF (264KB) ( 544 )   Save
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    To investigate the clinical effect of kidneybenefiting and conceptionaiding granules on infertility due to ovulatory dysfunction. Methods60 patients were randomly divided into two groups: the treatment group treated with kidneybenefiting and conceptionaiding granules and the control group treated with clomiphene citrate. They were examined by radioimmunoassay for female hormones such as　follicle stimulating hormone （FSH）， Luteinizing Hormone (LH), Estradiol (E2)， prolactin (PRL), testosterone(T), and Progesteron (P), and examined by B ultrasound to monitor the development of follicles and the endometrium before and after treatment. Results ① The pregnancy rate of the control group was 56.7%, while that of the treatment group was 33.3%(P＜0.05). ② The size of follicles and the depth of the endometrium were significantly improved after treatment in the treatment group, which was significantly different from the control group(P＜0.05). ③ The FSH, LH, E2, and P levels significantly increased and the PRL level decreased in both groups, which was not significantly different between the two groups（P＞0.05）． Conclusion① Kidneybenefiting and conceptionaiding granules are effective in the treatment of infertility due to ovulatory dysfunction and can significantly improve the pregnancy rate. ② Decoction regulates the HypothalamusPituitaryOvaryUterine Axis and promotes follicular development and endomembrane　thickening.
    Clinical features and MRI findings of multiple system atrophy
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  85-87. 
    Abstract ( 1031 )   PDF (247KB) ( 275 )   Save
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    To explore the clinical features of multiple system atrophy (MSA) and the significance of magnetic resonance imaging (MRI) in diagnosis. MethodsThe clinical data of 32 cases were retrospectively analyzed. ResultsThere were obvious cerebellar symptoms in MSAc with pontine, middle cerebellar peduncles, cerebellar vermis atrophy, fourth ventricle dilatation and T2WI high intensity in the pontocerebellar and middle cerebellar peduncle regions in MRI, which were significantly in accord with the presences of cerebellar dysfunction. Parkinsonism was found in MSAp with apparent basal ganglia changes such as putamen atrophy and T2WI high intensity of the lateral margin of the putamen. But the hyperintense rim on the T2W image had no relationship to the presence of Parkinsonism. ConclusionAllaround analysis of clinical features and MRI findings may help to precisely diagnose MSA and its subtypes.
    Aerobic exercise protects rats with chronic unpredictable mild stress against acute stress
    LIU Xin， YANG Lejin， FAN Shujuan, JIANG Hong, PAN Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  88-91. 
    Abstract ( 1227 )   PDF (291KB) ( 436 )   Save
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    To explore the protection of aerobic exercise on behavior and endocrine immunity in chronic unpredictable mild stress (CUMS) rats exposed to acute stress. Methods29 healthy male Wistar rats were randomly divided into four groups: the control group, the CUMS model group, the stress with aerobic exercise group, and the stress with labetalol group. Models were produced by a chronic unpredictable mild stress of 21 days. Aerobic exercise and labetalol were used for antistress. After the process of 21 days′ CUMS, acute stress was given. All the rats′ openfield activities were investigated, and serum cortisol and IL1β concentrations were determined by radioimmunoassays. ResultsBehaviors of the CUMS model group had depressive changes. Aerobic exercise not only improved the behavior of CUMS rats but also increased the serum cortisol to a quite normal level, which was higher than that of the CUMS model group and the labetalol group. Labetalol improved the crossings of CUMS rats, while other behavior indexes changed to the contrary. There was no statistical discrepancy of IL1β among groups. ConclusionAerobic exercise could protect CUMS rats against acute stress, which is much better than adrenergic receptor antagonist.
    Effect of adenotonsillectomy on behavior disorders in sleepdisordered breathing children
    FU Li, WANG Yan, LI Yanzhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  92-94. 
    Abstract ( 858 )   PDF (258KB) ( 260 )   Save
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    To explore the effect of adenotonsillectomy on behavior disorders in sleepdisordered breathing children. Methods65 children were monitored all night by polysomnography, and their behaviors were assessed by Conners concise symptom questionnaires. ResultsThe ratio of behavior disorder in OSAHS and primary snoring children was higher than that in healthy children, but there was no statistical different between the OSAHS group and the primary snoring group. After adenotonsillectomy the score in Conners concise symptom questionnaires of OSAHS children decreased from 14.2±3.8 to 6.7±3.1(P＜0.05) and of primary snoring children decreased from 9.4±4.1 to 5.1±2.8(P＜0.05). Behavior disorders were found in 19(52.3%) children in OSAHS children before surgeries but in 6(16.7%) children after adenotonsillectomy (χ2=4.347?8，　P＜0.05), and they were found in 14(48.3%) primary snoring children before surgeries and in 4 (13.8%) after adenotonsillectomy (χ2=5.263?2, P＜0.05). ConclusionThere are a lot of behavior disorders in OSAHS and primary snoring children. Adenotonsillectomy has some effect on them.
    Effect of Luohanshen on the feces excretion function in mice
    LIU Bin, XU Guifa, HAN Wenting, ZHAO Teng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  95-98. 
    Abstract ( 1179 )   PDF (247KB) ( 121 )   Save
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    To explore the effect of Luohanshen produced from Shan County of Shandong Province on the feces excretion function in mice. MethodsMice were randomly divided into the small intestine motor experiment group and the defecation experiment group. Then each group was randomly divided into five equal groups (the blank control group, the model control group, the low dose group, the middle dose group and the high dose group). Mice of the model control group and three dose groups had the constipation model established with compound Diphenoxylate after 15 days feeding with basal diet or basal diet containing 5%, 10% or 15% Luohanshen. The effect of Luohanshen on the feces excretion function in the constipated model mice was studied by small intestine motor experiments and defecation experiments. Defecation function of the normal mice was assessed in the blank control group and three dose groups of defecation experiment group after feeding three more days. ResultsThe small intestine motor experiments showed that the inkimpelling ratio of the middle and high dose groups was increased compared with the model control group(P＜0.01, P＜0.05). The defecation experiment on the constipation model mice showed that the time of the first ink defecation in the three dose groups was shorter than that of the control group(P＜0.01), the quantity and the weight of feces of the low and middle dose groups were higher than those of the control group in 5 hours(P＜0.05). The defecation experiment on normal mice showed that except for the time of the first ink defecation, no significant differences were found from the blank control group(P＜0.05). ConclusionThe results indicate that Luohanshen has a positive effect on purgation in constipated mice but has no effect in normal mice.
    Community structure and niche characteristics of hemorrhagic fever with renal syndrome host rats in a small environment
    BI Xiujuan1, SHAO Qi1, ZHANG Yingqian2, WANG Zhiqiang3, WANG Jiezhen1, XUE Fuzhong1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  99-102. 
    Abstract ( 1188 )   PDF (282KB) ( 333 )   Save
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    To analyze the community structure and niche characteristics of hemorrhagic fever with renal syndrome (HFRS) host rats. MethodsFive indexes, ShannonWeaner diversity value， Pielou uniformity value， dominance value， Levins niche width value and Petraitis niche overlap value were calculated to describe the community structure and niche characteristics of the HFRS host rats in the experimental districts. Results① The community structure of host rats was different in the experimental districts. The ShannonWeaner diversity value was lower in the first experimental district than in the second (P＜005), whereas the Pielou uniformity value and dominance value had no statistical significances(P＞005). ② Both the temporal niche breadth and the spatial niche breadth of Rattus norvegicus and Mus musculus were high, and their time niche overlap index and spatial niche overlap index were the highest, and others also had the ability to adapt to the environment. ConclusionThe ecological environment influences community structure of host rats in an experimental district. Rattus norvegicus and Mus musculus are the major species and they dominates both in temporal niche and in spatial niche.
    Interaction between polymorphism in the ADH1B gene and hypertriglyceridemia in premature coronary artery disease
    WANG Lili, LIU Zhaolan, FANG Shengxia, JIA Chongqi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(02):  103-105. 
    Abstract ( 925 )   PDF (265KB) ( 252 )   Save
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    To assess the interaction between the ADH1B*1/2 polymorphism and hypertriglyceridemia (HTG) in premature coronary artery disease (pCAD). MethodsA hospitalbased casecontrol study was conducted. Newly diagnosed CAD patients were recruited as the subjects. The 167 CAD patients diagnosed before or at 55 years old for males and 65 years old for　females were assigned as the pCAD, i.e. the case group; the other 235 lateonset CAD patients served as the control group. TG over 2.26?mmol/L was defined as hypertriglyceridemia. Polymerase chain reactionrestriction fragment length polymorphism was used to detect the polymorphisms. A multivariate logistic regression model was performed to adjust the potential confounders and estimate odds ratios (ORs). Synergy index (S), relative excess risk due to interaction (RERI) and attributable proportion due to interaction (AP) were the measures to assess the interaction as departure from additivity. ResultsThere was a positive interaction between the ADH1B*1/2 polymorphism and HTG in pCAD. S was 1.72, RERI was 1.78 and AP was 33.77%. After adjusting for sex, smoking, alcohol drinking, BMI, total cholesterol and diastolic blood pressure by multiple logistic regression, there was also a positive interaction. S was 2.96, RERI was 3.01 and AP was 54.29%. ConclusionThe interaction between the ADH1B*1/2 polymorphism and HTG had a positive effect on pCAD in this studied population.
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