Journal of Shandong University (Health Sciences) ›› 2020, Vol. 1 ›› Issue (7): 60-66.doi: 10.6040/j.issn.1671-7554.0.2020.0538

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Correlation of the expression of insulin-like growth factor II in granulosa cells and ovulation induction outcomes of 84 patients with polycystic ovary syndrome

DING Xiangyun1, YU Qingmei1, ZHANG Wenfang2, ZHUANG Yuan1, HAO Jing3   

  1. 1. Department of Histology and Embryology, Shandong Medical College, Linyin 276000, Shandong, China;
    2. Department of Reproductive Medicine, Linyi Women and Childrens Health Hospital, Linyin 276000, Shandong, China;
    3. Department of Anatomy and Histoembryology, School of Basic Medical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Online:2020-07-20 Published:2020-07-10

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Abstract: Objective To detect the expression of insulin-like growth factor II(IGF-II)in ovarian granulosa cells of patients with polycystic ovary syndrome(PCOS)and to explore its relationship with ovulation induction outcomes. Methods A total of 84 PCOS patients divided into high response group(n=42)and normal response group(n=42)and 90 controls(the control group)with assisted reproduction treatment due to tubal or male factors were enrolled. The expression of IGF-II in each group was detected with real-time quantitative reverse transcription PCR(qRT-PCR). The differences in IGF-II expressions and ovulation induction related parameters were compared, and the correlation was analyzed. Results The median relative expressions of IGF-II in the control group, PCOS normal response group and PCOS high response group were 1.3(0.5-1.8), 1.7(1.4-2.2)and 3.2(2.5-4.3), respectively. There were statistically significant differences among the three groups(F=88.7, P<0.001). The expression of IGF-II was higher in PCOS normal response group and PCOS high response group than that in the control group(both P<0.001), and the expression of IGF-II was higher in PCOS high response group than in PCOS normal response group(P<0.001). On the day when human chorionic gonadotrophin(HCG)was injected, in PCOS high response group, estradiol(E2)level, number of follicles ≥14 mm, number of oocytes obtained, number of 2 pronucleus(2PN)zygotes, number of high quality embryos, number of cleavages and number of transplantable embryos were significantly higher than those in the control group and PCOS normal response group(P<0.05); however, the luteotropic hormone(LH)level, oocyte recovery rate, fertilization rate and available oocyte rate were significantly lower than those in the control group and PCOS normal response group(P<0.05). In the PCOS normal response group, gonadotropin(Gn)level(P=0.02)and E2 level(P<0.001)were lower than those in the control group. Correlation analysis showed that the expression of IGF-II was positively correlated with E2 level(r=0.163, P=0.032), number of follicles ≥14 mm on HCG day(r=0.286, P<0.001), number of 2PN(r=0.246, P<0.001), number of cleavages(r=0.296, P<0.001), number of high quality embryos(r=0.286, P<0.001), number of oocytes obtained(r=0.386, P<0.001), number of transplantable embryos(r=0.252, P<0.001), and rate of high-quality embryos(r=0.155, P=0.041). Multivariate linear regression analysis revealed that the expression of IGF-II was positively correlated with the number of oocytes obtained(β=0.921, P<0.001)and rate of high-quality embryos(β=0.285, P<0.001), while negatively correlated with number of cleavages(β=-0.283, P<0.001). Conclusion IGF-II is highly expressed in the granulosa cells of PCOS patients undergoing assisted reproductive technology, which is closely related to the number of oocytes obtained and rate of high-quality embryos, and has the potential to promote the development of follicles and early embryos.

Key words: Insulin-like growth factor II, Polycystic ovary syndrome, Granulosa cell, Induced ovulation

CLC Number: 

  • R711.75
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