JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 77-80,95.doi: 10.6040/j.issn.1671-7554.0.2014.360

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Expression and significance of GDF9 and BMP15 in follicular fluid of women with polycysticovary syndrome

HA Lingxia1,2, LI Xianghong1,2   

  1. 1. Center for Reproductive Medicine, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    2. Key Laboratory of Reproduction and Genetics, Key Laboratory of Fertility Preservation and Maintenance, Ningxia Medical University, Ministry of Education in Ningxia, Yinchuan 750004, Ningxia, China
  • Received:2014-06-01 Revised:2014-09-12 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To investigate the expression of growth differentiation factor9 (GDF9) and bone morphogenetic protein15 (BMP15) in follicular fluid of polycystic ovarian syndrome (PCOS) patients and the effects on the outcome of in vitro fertilization and embryo transfer (IVF-ET). Methods A total of 60 PCOS patients (PCOS group) and 60 women with normal ovarian function (control group) were collected. The general information, the numbers of oocytes, mature oocytes, fertilized oocytes, cleavage, high quality embryos, and the rate of clinical pregnancy were recorded. Western blotting was used to detect the expressions of GDF9 and BMP15 in the follicular fluid of all subjects. The PCOS group was further divided into pregnant and non-pregnant subgroups according to the presence of a gestational sac on ultrasound. The expressions of GDF9 and BMP15 were analyzed. Results ① The number of oocytes was 18.76±8.51 and 11.23±4.29 in the PCOS group and control group, with significant difference (P<0.05). The number ofmature eggs, fertilization rate, cleavage rate, good quality embryo and the clinical pregnancy rate between the two groups had no significant differences (P>0.05). ② The expressions of GDF9 and BMP15 were 0.85±0.27 and 1.29±0.48 in the PCOS group, and 1.33±0.24 and 1.94±0.57 in the control group, with significant difference (P<0.05). ③ The expressions of GDF9 and BMP15 were 1.01 ± 0.31 and 1.50 ± 0.41 in the pregnant subgroup, and 0.71±0.21 and 1.09±0.46 in the non-pregnant subgroup, with significant difference (P<0.05). Conclusion The expressions of GDF9 and BMP15 in follicular fluid in the PCOS group are lower than those of the control group, suggesting that the low expression may affect oocyte quality and embryo development potential. The expressions of GDF9 and BMP15 in follicular fluid of the pregnant subgroup are higher than those of the non-pregnant subgroup, suggesting that the high expression may be associated with a good pregnancy outcome.

Key words: Polycystic ovary syndrome, In vitro fertilization and embryo transfer, Growth differentiation factor 9, Bone morphogenetic protein 15

CLC Number: 

  • R711.6
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