Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (2): 75-79.doi: 10.6040/j.issn.1671-7554.0.2018.831

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Expression of Forkhead box protein O1 mRNA in granulosa cells of polycystic ovary sydrome patients and its significance

HE Tingting, LIU Xin, LIU Hongbin, ZHAO Shigang, ZHANG Xiuqing, LIU Qingqing, YOU Li, SHI Yuhua   

  1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    The Key Laboratory of Reproductive Endocrinology(Shandong University), Ministry of Education;
    Shandong Provincial Clinical Medicine Research Center for Reproductive Health, Jinan 250001, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the expression and significance of Forkhead box protein O1(FOXO1)in ovarian granulosa cells of patients suffering from polycystic ovary syndrome(PCOS). Methods A total of 130 patients, including 67 PCOS patients and 63 controls, were enrolled. The expression of FOXO1 in ovarian granulosa cells was examined by real-time quantitative reverse transcription PCR(qRT-PCR), and the clinical significance was analyzed. Results PCOS patients had significantly higher body mass index(BMI), homeostasis model assessment-insulin resistance(HOMA-IR), luteotropic hormone(LH), testosterone(T), anti-Mullerian hormone(AMH), average follicle count(AFC)levels(all P<0.05), but lower FSH level(P<0.05)than controls. Compared with controls, the expression of FOXO1 was much lower in granulosa cells of PCOS patients(P<0.05). Binary Logistic regression analysis revealed that FOXO1, AFC and HOMA-IR were risk factors influencing the occurrence of PCOS(P<0.05). Conclusion The lower expression of FOXO1 in the granulosa cells of PCOS patients may play an important role in the occurrence and development of PCOS.

Key words: Polycystic ovary syndrome, Granulosa cell, Forkhead box protein O1

CLC Number: 

  • R711.75
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