Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (1): 48-54.doi: 10.6040/j.issn.1671-7554.0.2018.726

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Expression of polycystic ovary syndrome susceptibility gene dennd1a in zebrafish models

LIU Congcong, LIU Hongbin, FU Jiang, LI Lei   

  1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    Key Laboratory for Reproductive Endocrinology (Shandong University), Ministry of Education;
    Shandong Provincial Key Laboratory of Reproductive Medicine, Shandong Provincial Clinical Medicine Research Center for Reproductive Health, Jinan 250001, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To study the expression characteristics of polycystic ovary syndrome(PCOS)susceptibility gene dennd1a in developing embryos and in adult organs of zebrafish, and to establish zebrafish models for studies of the mechanisms underlying PCOS. Methods Using reverse transcription-polymerase chain reaction(RT-PCR)and real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR)techniques, we detected the expression of dennd1a in zebrafish. We also determined the expression pattern of dennd1a gene in zebrafish embryos using whole-mount in situ hybridization(WISH)assays. Results In embryos and juveniles, the expression level of dennd1a increased with age, and in adult animals, the expression was found in different organs. qRT-PCR and WISH showed the expression of dennd1a especially in the nervous system and male reproductive system. Conclusion In early stages of zebrafish development, high expression of dennd1a was found in the nervous system and male reproductive system, indicating this gene may regulate the development and function of the endocrine system and interrupt androgen synthesis and release, thereby inducing PCOS.

Key words: Polycystic ovary syndrome, dennd1a, Gene expression, Zebrafish, Animal models

CLC Number: 

  • R711.75
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